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Effect of wheat powdery mildew on grain nitrogen metabolism

Published online by Cambridge University Press:  21 April 2021

H. Y. Gao Open the ORCID record for H. Y. Gao [Opens in a new window] ,
J. S. Niu ,
W. Q. Liu ,
D. L. Zhang  and
S. P. Li
H. Y. Gao
Affiliation:
School of Life Sciences of Henan University, Kaifeng475004, China School of Life Sciences of Zhengzhou Normal University, Zhengzhou450044, China
J. S. Niu
Affiliation:
National Centre of Engineering and Technological Research for Wheat, Henan Agricultural University, Zhengzhou450046, China
W. Q. Liu
Affiliation:
Zhengzhou City Fulun Foreign Language School, Zhengzhou450000, China
D. L. Zhang
Affiliation:
School of Life Sciences of Henan University, Kaifeng475004, China
S. P. Li*
Affiliation:
School of Life Sciences of Henan University, Kaifeng475004, China
*
Author for correspondence: S. P. Li, E-mail: [email protected]
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Abstract

Glutamine synthetase (GS) and glutamate synthase (GOGAT) play a central role in plant nitrogen (N) metabolism. In order to study the effect of powdery mildew (Blumeria graminis f. sp. tritici, Bgt) on N metabolism, field experiments were carried out to evaluate GS and GOGAT activity, GS expression and grain protein content (GPC) in susceptible (Xi'nong 979) and resistant (Zhengmai 103) wheat cultivars under three treatments. The three treatments were no inoculation (CK), inoculated once with Bgt (MP) and inoculated nine times with Bgt (HP). For Xi'nong 979, the activities of GS and GOGAT in grains as well as GS activity in flag leaves increased at 10–15 days after anthesis (DAA), and decreased significantly at 15 or 20–30 DAA in HP and MP. However, GS activity in grains decreased from 20 DAA, which was later than that of flag leaves (15 DAA). At the same time, GS expression in grains was up-regulated at early stage, with GS1 at 10 DAA and GS2 at 15 DAA, followed by a continuous down-regulation. This result indicated that GS and GOGAT activity as well as GS expression were inhibited by powdery mildew, indicating that N metabolism in grains was inhibited at 20–30 DAA. The current study also found out that the yield of the susceptible cultivar decreased significantly, while its GPC increased obviously in HP. It was shown that the increase of GPC was not due to the enhancement of N metabolism, but due to the passive increase caused by yield reduction.

Keywords

ActivityBlumeria graminis f. sp. tritici (Bgt)gene expressionglutamate synthaseglutamine synthetase
Type
Crops and Soils Research Paper
Information
The Journal of Agricultural Science , Volume 159 , Issue 1-2 , January 2021 , pp. 128 - 138
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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