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The effect of urea on the utilization of ground, pelleted roughage by penned sheep: III. Mineral supplements

Published online by Cambridge University Press:  27 March 2009

J. B. Coombe ,
K. R. Christian  and
M. D. Holgate
M. D. Holgate
Affiliation:
Division of Plant Industry, C.S.I.R.O., Canberra, Australia
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Summary

Seven, groups of adult Merino wethers were fed for 16 weeks on a diet of pelleted oat straw and urea, either alone or supplemented with trace minerals and various combinations of the major minerals. In some treatments, the same diet was fed throughout; in others, diets were changed after 8 weeks.

Sulphur (S) was by far the most important mineral supplement; sheep fed diets supplemented with all minerals except S performed as poorly as those on the basal diet. Of the other minerals, only calcium (Ca) and magnesium (Mg), which were fed in combination, had a small, additional beneficial effect. Supplementary phosphorus (P) had no effect whether or not S was included.

Intakes of sheep on S-supplemented diets increased over the first 6 weeks to reach stable levels of 1200–1400 g D.M./day compared with approximately 800 g/day for controls, while O.M. digestibilities stabilized at about 40 and 33% for S-supplemented and unsupplemented diets respectively. Live-weight change and wool growth were closely related to intake. Mean live-weight losses during the first and second 8 weeks for sheep without supplementary S were 9·5 and 4·8 kg respectively, and were about 50% of this for supplemented sheep. Sheep which were changed from diets without supplementary S to S-supplemented diets after 8 weeks showed slight increases in live weight from then on. Wool growth was about 115 g clean wool/sheep on all treatments during the first month, after which it remained steady at about 105 g/month on S-supplemented diets and fell to 60 g/month on diets without supplementary S.

Sheep without supplementary S were in negative nitrogen (N) and S balance throughout. With the S-supplemented diets, urinary excretion of N and S was high, and few positive balances were recorded until the final 4 weeks of the experiment. Serum urea levels were high (60–130 mg/100 ml) throughout, and showed no effect of diet or period. Phosphorus balances were uniformly negative in sheep without supplementary P, and generally positive in P-supplemented sheep. Serum inorganic P levels were apparently affected by P intake and by live-weight loss, but mean values never fell below 4·5 mg/100 ml.

Sheep without supplementary Ca were in negative Ca balance throughout the experiment; however, Ca supplementation generally resulted in high faecal Ca losses and few positive balances were recorded. In some sheep, serum Ca fell to moderately low levels (7·5 mg/100 ml), but this appeared to be associated more with high serum inorganic P levels than low Ca intake or retention. All sheep were practically in Mg, Na and K balance throughout, and supplementation had little effect on the retention of these elements.

All sheep were in very poor condition at the end of the experiment, even when their D.M. intakes had been high, and wool growth rates were mediocre throughout. It appears that urea was poorly utilized in this experiment, and that satisfactory sheep production on diets of ground straw supplemented only with urea and minerals is unlikely.

Type
Short Note
Information
The Journal of Agricultural Science , Volume 77 , Issue 1 , August 1971 , pp. 159 - 174
Copyright
Copyright © Cambridge University Press 1971

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References

Agricultural Research Council. (1965). The nutrient requirements of farm livestock. 2. Ruminants. Technical Reviews and Summaries. London: Agricultural Research Council.Google Scholar
Albert, W. W., Garrigus, U. S., Forbes, R. M. & Norton, H. W. (1956). The sulfur requirement of growing fattening lambs in terms of methionine, sodium sulfate and elemental sulfur. J. Anim. Sci. 15, 559.CrossRefGoogle Scholar
Anderson, R., Cheng, E. & Burroughes, W. (1956). A laboratory technique for measuring phosphorus availability of feed supplements fed to ruminants. J. Anim. Sci. 15, 489.CrossRefGoogle Scholar
Armstrong, D. G. (1964). The evaluation of artificially dried grass as a source of energy for sheep. II. The energy value of cocksfoot, timothy and two strains of rye-grass at varying stages of maturity. J. agric. Sci., Camb. 62, 399.CrossRefGoogle Scholar
Armstrong, D. G., Blaxter, K. L. & Waite, R. (1964). The evaluation of artificially dried grass as a source of energy for sheep. III. The prediction of nutritive value from chemical and biological measurements. J. agric. Sci., Camb. 62, 417.CrossRefGoogle Scholar
Beal, A. M. & Budtz-Olsen, O. E. (1968). A potassium and sodium balance study in two breeds of sheep. Aust. J. agric. Res. 19, 113.CrossRefGoogle Scholar
Blaxter, K. L. (1964). Utilization of the metabolizable energy of grass. Proc. Nutr. Soc. 23, 62.CrossRefGoogle ScholarPubMed
Blaxter, K. L. & Wainman, F. W. (1964). The utilization of the energy of different rations by sheep and cattle for maintenance and for fattening. J. agric. Sci., Camb. 63, 113.CrossRefGoogle Scholar
Bloomfield, R. A., Garner, G. B. & Muhrer, M. E. (1960). Kinetics of urea metabolism in sheep. J. Anim. Sci. 19, 1248.Google Scholar
Bray, A. C. (1969). Sulphur metabolism in sheep. I. Preliminary investigations on the movement of sulphur in the sheep's body. Aust. J. agric. Res. 20, 725.CrossRefGoogle Scholar
Bray, A. C. & Hemsley, J. A. (1969). Sulphur metabolism in sheep. IV. The effect of a varied dietary sulphur content on some body fluid sulphate levels and on the utilization of urea-supplemented roughage by sheep. Aust. J. agric. Res. 20, 759.CrossRefGoogle Scholar
Briggs, A. P. (1922). A modification of the Bell-Doisy phosphate method. J. biol. Chem. 53, 13.CrossRefGoogle Scholar
Broad, A., Gillespie, J. M. & Reis, P. J. (1970). The influence of sulphur-containing amino acids on the biosynthesis of high-sulphur wool proteins. Aust. J. biol. Sci. 23, 149.CrossRefGoogle Scholar
Bryant, M. P., Robinson, I. M. & Chu, H. (1959). Observations on the nutrition of Bacteroides succinogenes–a ruminal cellulolytic bacterium. J. Dairy Sci. 42, 1831.CrossRefGoogle Scholar
Burroughs, W., Latona, A., De Paul, P., Gerlaugh, P. & Bethke, R. M. (1951). Mineral influences upon urea utilization and cellulose digestion by rumen microorganisms using the artificial rumen technique. J. Anim. Sci. 10, 693.CrossRefGoogle Scholar
Bushman, D. H., Emerick, R. J. & Embry, L. B. (1968). Effects of various chlorides and calcium carbonate on calcium, phosphorus, sodium, potassium and chloride balance and their relationship to urinary calculi in lambs. J. Anim. Sci. 27, 490.CrossRefGoogle ScholarPubMed
Caffrey, P. J., Hatfield, E. E., Norton, H. W. & Garrigus, U. S. (1967). Nitrogen metabolism in the ovine. I. Adjustment to a urea-rich diet. J. Anim. Sci. 26, 595.CrossRefGoogle ScholarPubMed
Chalupa, W. (1968). Problems in feeding urea to ruminants. J. Anim. Sci. 27, 207.CrossRefGoogle ScholarPubMed
Chalupa, W., Evans, J. L. & Stillions, M. C. (1964). Metabolic aspects of urea utilization by ruminant animals. J. Nutr. 84, 77.CrossRefGoogle ScholarPubMed
Chicco, C. F., Ammerman, C. B., Moore, J. E., Van Walleghem, P. A., Arrington, L. R. & Shirley, R. L. (1965). Utilization of inorganic ortho-, meta- and pyrophosphates by lambs and by cellulolytic rumen microorganisms in vitro. J. Anim. Sci. 24, 355.CrossRefGoogle ScholarPubMed
Coombe, J. B. & Christian, K. R. (1969). The effect of urea on the utilization of ground, pelleted roughage by penned sheep. II. Utilization of organic matter, nitrogen and minerals. J. agric. Sci., Camb. 72, 261.CrossRefGoogle Scholar
Coombe, J. B. & Preston, G. K. (1969). The effect of urea on the utilization of ground, pelleted roughage by penned sheep. I. Food intake, live-weight change and wool growth. J. agric. Sci., Camb. 72, 251.CrossRefGoogle Scholar
Coombe, J. B. & Tribe, D. E. (1962). The feeding of urea supplements to sheep and cattle: the results of penned feeding and grazing experiments. J. agric. Sci., Camb. 55, 125.CrossRefGoogle Scholar
Conrad, H. R. & Hibbs, J. W. (1968). Nitrogen utilization by the ruminant. Appreciation of its nutritive value. J. Dairy Sci. 51, 276.CrossRefGoogle ScholarPubMed
Dukes, H. H. (1955). The Physiology of Domestic Animals. 7th ed.Ithaca: Cornell University Press.Google Scholar
Duncan, D. B. (1955). Multiple range and multiple F tests. Biometrics 11, 1.CrossRefGoogle Scholar
Evans, J. L. & Davis, G. K. (1966). Dietary phosphorus, sulfur and molybdenum and mineral composition of rumen fluid. J. Anim. Sci. 25, 1010.CrossRefGoogle Scholar
Folin, O. (1933). Standardised methods for the determination of uric acid in unlaked blood and in urine. J. biol. Chem. 101, 111.CrossRefGoogle Scholar
Graham, N. McC. (1964). Energetic efficiency of fattening sheep. II. Effects of undernutrition. Aust. J. agric. Res. 15, 113.Google Scholar
Graham, N. McC. (1967). The net energy value of three subtropical forages. Aust. J. agric. Res. 18, 137.Google Scholar
Graham, N. McC. (1969). Relation between metabolizable and digestible energy in sheep and cattle. Aust. J. agric. Res. 20, 1117.CrossRefGoogle Scholar
Griffith, W. H. (1926). Benzoylated amino acids in the animal organism. III. A method for the determination of hippuric acid and study of the synthesis of hippuric acid in rabbits. J. biol. Ghem. 69, 197.CrossRefGoogle Scholar
Hemingway, R. G. (1967). Phosphorus and the ruminant. Outl. Agric. 5, 172.CrossRefGoogle Scholar
Hemsley, J. A. (1966). Dietary cellulose content and urea utilization. Proc. Aust. Soc. Anim. Prod. 6, 384.Google Scholar
Hemsley, J. A. (1968). Relative values of urea and protein as nitrogen supplements for a low-quality roughage. Proc. Aust. Soc. Anim. Prod. 7, 381.Google Scholar
Hoar, D. W., Emerick, R. J. & Embry, L. B. (1969). Ovine phosphatic urolithiasis as related to the phosphorus and calcium contents and acid-baseforming effects of all-concentrate diets. J. Anim. Sci. 29, 647.CrossRefGoogle Scholar
Hogan, J. P. & Weston, R. H. (1967). The digestion of chopped and ground roughages by sheep. II. The digestion of nitrogen and some carbohydrate fractions in the stomach and intestines. Aust. J. agric. Res. 18, 803.CrossRefGoogle Scholar
Hubbert, F. Jr, Cheng, E. & Burroughs, W. (1958a). Mineral requirements of rumen microorganisms for cellulose digestion in vitro. J. Anim. Sci. 17, 559.CrossRefGoogle Scholar
Hubbert, F. Jr, Cheng, E. & Burroughs, W. (1958b). The influence of potassium, sodium, rubidium, lithium and cesium on in vitro cellulose digestion byrumen microorganisms with observations upon sodium and potassium influences in lamb fattening rations. J. Anim. Sci. 17, 576.CrossRefGoogle Scholar
Hume, I. D. (1970). Synthesis of microbial protein in the rumen. III. The effect of dietary protein. Aust. J. agric. Res. 21, 305.CrossRefGoogle Scholar
Hume, I. D. & Bird, P. R. (1970). Synthesis of microbial protein in the rumen. IV. The influence of the level and form of dietary sulphur. Aust. J. agric. Res. 21, 315.CrossRefGoogle Scholar
Hume, I. D., Moir, R. J. & Somers, M. (1970). Synthesis of microbial protein in the rumen. I. Influence of the level of nitrogen intake. Aust. J. agric. Res. 21, 283.CrossRefGoogle Scholar
Hungate, R. E. (1966). The Rumen and Its Microbes. New York: Academic Press.Google Scholar
Khan, B. B., Nagy, J. G. & Ward, G. M. (1969). Effects of potassium levels on rumen microbial activity. J. Anim. Sci. 28, 859.Google Scholar
Kirchgessner, M. (1965). Availability of some trace elements. Proc. Nutr. Soc. 24, 89.CrossRefGoogle ScholarPubMed
Lewis, D. (1957). Blood-urea concentration in relation to protein utilization in the ruminant. J. agric. Sci., Camb. 48, 438.CrossRefGoogle Scholar
Lines, E. W. & Peirce, A. W. (1931). The basal (standard) metabolism of the Australian Merino sheep. Bull. Commonw. scient. ind. Res. Org. no. 55.Google Scholar
Loosli, J. K., Williams, H. H., Thomas, W. E., Ferris, F. H. & Maynard, L. A. (1949). Synthesis of amino acids in the rumen. Science, N.Y. 110, 144.CrossRefGoogle ScholarPubMed
McLaren, G. A., Anderson, G. C., Welch, J. A., Campbell, C. D. & Smith, G. S. (1959). Diethylstilbestrol and length of preliminary period in the utilization of crude biuret and urea by lambs. I. Digestion and nitrogen retention. J. Anim. Sci. 18, 1319.CrossRefGoogle Scholar
McNaught, M. L., Owen, E. C. & Smith, J. A. B. (1950). The utilization of non-protein nitrogen in the bovine rumen. 6. The effect of metals on the activity of the rumen bacteria. Biochem. J. 46, 36.CrossRefGoogle ScholarPubMed
Meier, H. (1963). Fluids and electrolytes. Ch. 12 in Clinical Biochemistry of Domestic Animals, ed. by Cornelius, C. E. and Kaneko, J. J.. New York: Academic Press.Google Scholar
Modjanov, A. V. (1959). Conditions for effective use of urea in feeding sheep. Vestn. sel' skohoz. Nanki. no. 10, 47, cited in Nutr. Abstr. Rev. (1960). 30, 669.Google Scholar
Moir, R. J., Somers, M. & Bray, A. C. (1967). Utilization of dietary sulphur and nitrogen by ruminants. Sulphur Inst. J. 3, 15.Google Scholar
National Research Council (1964). Nutrient Requirements of Domestic Animals. No. V. Nutrient Requirements of Sheep, 3rd rev. ed. Washington: National Academy of Sciences – National Research Council.Google Scholar
Nehring, K. & Zelck, U. (1965). Über die Zusammensetzung des Harns an organischen Inhaltsstoffen bei Rinder, Schafen and Schweinen. I. Zur Analytik von organischen Harninhaltsstoffen. Arch. Tierernähr. 15, 25.CrossRefGoogle Scholar
Oltjen, R. R. (1969). Effects of feeding ruminants non-protein nitrogen as the only nitrogen source. J. Anim. Sci. 28, 673.CrossRefGoogle ScholarPubMed
Oltjen, R. R., Bond, J. & Richardson, G. V. (1969). Growth and reproductive performance of bulls and heifers fed purified and natural diets. I. Performance from 14 to 189 days of age. J. Anim. Sci. 28, 717.CrossRefGoogle Scholar
Owen, J. B., Miller, E. L. & Bridge, P. S. (1969). Complete diets given ad libitum to dairy cows – the effect of the level of inclusion of milled straw. J. agric. Sci., Camb. 72, 351.CrossRefGoogle Scholar
Packett, L. V., Lineberger, R. O. & Jackson, H. D. (1968). Mineral studies in ovine phosphatic urolithiasis. J. Anim. Sci. 27, 1716.CrossRefGoogle ScholarPubMed
Paladines, O. L., Reid, J. T., Bensadoun, A. & Van Niekerk, B. D. H. (1964). Heat of combustion values of the protein and fat in the body and wool of sheep. J. Nutr. 82, 145.CrossRefGoogle ScholarPubMed
Pearson, R. M. & Smith, J. A. B. (1943). The utilization of urea in the bovine rumen. 2. The conversion of urea to ammonia. Biochem. J. 37, 148.CrossRefGoogle ScholarPubMed
Pickard, D. W., Swan, H. & Lamming, G. E. (1969). Studies on the nutrition of ruminants. 4. The use of ground straw of different particle sizes for cattle from twelve weeks of age. Anim. Prod. 11, 543.Google Scholar
Preston, T. R., Elias, A., Willis, M. B. & Sutherland, T. M. (1967). Intensive beef production from molasses and urea. Nature, Lond. 216, 721.CrossRefGoogle Scholar
Reis, P. J. (1965). The growth and composition of wool. III. Variations in the sulphur content of wool. Aust. J. Biol. Sci. 18, 671.CrossRefGoogle Scholar
Schelling, G. T., Hinds, F. C. & Hatfield, E. E. (1967). Effect of dietary protein levels, amino acid supplementation and nitrogen source upon the plasma free amino acid concentrations in growing lambs. J. Nutr. 92, 339.CrossRefGoogle ScholarPubMed
Smith, G. S., Dunbar, R. S., McLaren, G. A., Anderson, G. C. & Welch, J. A. (1960). Measurement of the adaptation response to urea-nitrogen utilization in the ruminant. J. Nutr. 71, 20.CrossRefGoogle ScholarPubMed
Starks, P. B., Hale, W. H., Garrigus, U. S., Forbes, R. M. & James, M. F. (1954). Response of lambs fed varied levels of elemental sulfur, sulfate sulfur, and methionine. J. Anim. Sci. 13, 249.CrossRefGoogle Scholar
Swan, H. & Lamming, G. E. (1968). Studies on the nutrition of ruminants. 3. The substitution of soya bean meal by urea in diets containing ground roughage and cereal. Anim. Prod. 10, 193.Google Scholar
Thomas, W. E., Loosli, J. K., Williams, H. H. & Maynard, L. A. (1951). The utilization of inorganic sulfates and urea nitrogen by lambs. J. Nutr. 43, 515.CrossRefGoogle ScholarPubMed
Thornton, R. F. (1970). Factors affecting the urinary excretion of urea nitrogen in cattle. II. The plasma urea nitrogen concentration. Aust. J. agric. Res. 21, 145.CrossRefGoogle Scholar
Tomas, F. M., Moir, R. J. & Somers, M. (1967). Phosphorus turnover in sheep. Aust. J. agric. Res. 18, 635.CrossRefGoogle Scholar
Topps, J. H. & Elliott, R. C. (1965). Relationship between concentration of ruminal nucleic acids and excretion of purine derivatives by sheep. Nature, Lond. 205, 498.CrossRefGoogle Scholar
Topps, J. H. & Elliott, R. C. (1967). Partition of nitrogen in the urine of African sheep given a variety of low-protein diets. Anim. Prod. 9, 219.Google Scholar
Trenkle, A., Cheng, E. & Burroughs, W. (1958). Availability of different sulfur sources for rumen microorganisms in in vitro cellulose digestion. J. Anim. Sci. 17, 1191.Google Scholar
Underwood, E. J. (1966). The Mineral Nutrition of Livestock. Commonwealth Agricultural Bureau, by arrangement with F.A.O.Google Scholar
Virtanen, A. I. (1966). Milk production of cows on protein-free feed. Studies of the use of urea and ammonium salts as the sole nitrogen source open new important perspectives. Science, N.Y. 153, 1603.CrossRefGoogle Scholar
Visek, W. J. (1968). Some aspects of ammonia toxicity in animal cells. J. Dairy Sci. 51, 286.CrossRefGoogle ScholarPubMed
Waldo, D. R. (1968). Symposium: nitrogen utilization by the ruminant. Nitrogen metabolism in the ruminant. J. Dairy Sci. 51, 265.CrossRefGoogle ScholarPubMed
Weston, R. H. & Hogan, J. P. (1968). Rumen ammonia in relation to characteristics of the diet and parameters of nitrogen metabolism. Proc. Aust. Soc. Anim. Prod. 7, 359.Google Scholar
White, R. R., Christian, K. R. & Williams, V. J. (1956). Blood chemistry and haematology in sheep on decreasing levels of feed intake followed by starvation. N.Z. Jl Sci. Technol. A 38, 440.Google Scholar
Wootton, K. D. P. (1964). Micro-analysis in Medical Biochemistry, 4th ed.London: J. and A. Churchill.Google Scholar