Four near-isogenic lines of wheat were employed to compare the effects of endosperm texture (hard v. soft) and the presence or absence of the 1B/1R translocation on amino acid digestion as determined with young broilers. Wheat samples were incorporated into diets at rates of inclusion of 250, 500 and 750 g/kg, with wheat as the only protein source. Diets included a mineral/vitamin mixture (50 g), oil (50 g) and were made up to 1000 g with a 50[ratio ]50 starch[ratio ]glucose mixture. Titanium dioxide was included at 5 g/kg as an inert marker. Each of the 12 diets was fed to 6 pairs of growing male (Ross) chickens for 3 days. Samples of ileal digesta were obtained from the birds following slaughter at 21 or 22 days of age and analysed for amino acid and titanium dioxide content. True and apparent digestibility coefficients were determined by regressing the amount of dietary apparently digestible amino acid in the diet against the rate of wheat inclusion and extrapolating the response to 1000 g and 0 g wheat/kg. The ordinate intercept was assumed to represent endogenous losses. Both the presence of the translocation and hard endosperm texture were associated with decreases in the apparent digestibility of amino acids, although the latter characteristic was not as important. As examples, for methionine the translocation was associated with a decrease in the coefficient of digestibility from 0·872 to 0·802 and a change in endosperm texture from soft to hard was associated with a decrease from 0·845 to 0·829.