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The effect of interval between harvests and nitrogen application on the proportion and digestibility of cell wall, cellulose, hemicellulose and lignin and on the proportion of lignified tissue in leaf cross-section in two perennial ryegrass varieties

Published online by Cambridge University Press:  27 March 2009

D. Wilman ,
M. Daly ,
A. Koocheki  and
A. B. Lwoga
D. Wilman
Affiliation:
Department of Agriculture, University College of Wales, Aberystwyth
M. Daly
Affiliation:
Department of Agriculture, University College of Wales, Aberystwyth
A. Koocheki
Affiliation:
Department of Agriculture, University College of Wales, Aberystwyth
A. B. Lwoga
Affiliation:
Department of Agriculture, University College of Wales, Aberystwyth
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Summary

The effect of six intervals between harvests and two levels of N application on the proportion and digestibility of cell wall was studied in two perennial ryegrass (Lolium perenne) varieties in the first and second harvest years in a field experiment. In the oase of four of the six intervals between harvests the proportion and digestibility of cellulose, hemicellulose and lignin and the N content of cell wall were determined. Diets derived from three of the harvesting treatments were fed, at maintenance level, to sheep as a partial in vivo check on the in vitro procedures. The effect of six intervals between harvests and two levels of N application on the proportion of lignified tissue in leaf cross-section was determined in two varieties in the first harvest year.

Effects of interval between harvests and time of year on herbage digestibility were due to effects on both proportion and digestibility of cell wall, particularly the latter. Hemicellulose digestibility appeared to be rather more affected by interval between harvests and rather more closely associated with the proportion of lignin than was cellulose digestibihty. The proportion of cell wall was particularly low and its digestibility particularly high in April and early May.

The N in cell wall accounted for about 10% of total N in harvested herbage on all treatments. The ratio of cellulose to hemicellulose averaged 1:0·88 and hemicellulose was rather more digestible than cellulose. Cellulose and cell wall were less digestible in vitro than in vivo.

The application of N tended to reduce the proportion of cell wall and to increase its digestibility in leafy but not in stemmy crops.

Interval between harvests and N application had no effect on the proportion of lignified tissue in leaf cross-section.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 89 , Issue 1 , August 1977 , pp. 53 - 63
Copyright
Copyright © Cambridge University Press 1977

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