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Effect of feeding protected lipid to dairy cows in early lactation on the composition of blood lipoproteins and secretion of fatty acids in milk

Published online by Cambridge University Press:  27 March 2009

J. E. Storry ,
P. E. Brumby ,
B. Tuckley ,
V. A. Welch ,
D. Stead  and
Rosemary J. Fulford
J. E. Storry
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading, RG2 9AT
P. E. Brumby
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading, RG2 9AT
B. Tuckley
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading, RG2 9AT
V. A. Welch
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading, RG2 9AT
D. Stead
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading, RG2 9AT
Rosemary J. Fulford
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading, RG2 9AT
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Summary

Effects of 0, 1·7, 3·3 or 5·0 kg/day of a protected soya bean – tallow supplement, incorporated into a hay:concentrate diet (25:75) and fed ad libitumto Friesian cows, on intake and digestion of fatty acids, on output of milk fatty acids and on blood lipoprotein composition were measured.

Most of the increased intake of fatty acids, approximately 1 kg/day, was accounted for by increased intakes of C16:0, C18:0 and C18:1. At low intakes, amounts of all fatty acids apparently digested were linearly related to their respective intakes. At high intakes of C16 and C18 acids, curvilinear relationships were established.

Yield of total milk fat was related positively to dietary intakes of total fatty acid and carbohydrate and negatively to live-weight change. Yields of short and intermediate chain acids in milk, synthesized within the mammary gland, were negatively correlated and yields of C18 fatty acids positively correlated with respective dietary intakes of these acids. Decreased proportions of C4–16 and increased proportions of C18:0 and C18:1 fatty acids in milk were associated with increased protected tallow in the diet. Yields of C16:1 and C18:1 were positively related to corresponding outputs of saturated acids and negatively to weeks of lactation. The proportion of C18:1 in milk was positively related to the corresponding proportion of C18:0.

The increased intake of fatty acids resulted in increased concentrations of very low density lipoproteins (VLDL, d < 1·019 g/ml), low density lipoproteins (LDL1 + LDL2, 1·019 < d < 1·06 g/ml), high density lipoproteins (d > 1·060 g/ml) and serum free fatty acids. Most of the increase in low density lipoproteins was accounted for by a very large increase in LDL1, whose proportion increased from 17 to 75% (2 to 22% of total serum lipid). The proportion of triglyceride in the combined low density lipoprotein fraction decreased from 11 to 2% whilst phospholipids increased from 29 to 36%. These changes were attributed to the increased proportion of LDL1 present.

The proportions of VLDL and LDL triglyceride taken up by the mammary gland averaged 0·79 and 0·34 respectively. The proportion of VLDL+LDL triglyceride taken up by the gland decreased with increased amounts of fatty acid digested. Yields of C18 fatty acids in milk tended to be positively related to apparent uptakes of VLDL triglyceride and to VLDL C18 fatty acids, but negatively related to apparent uptakes of LDL triglycerides and LDL C18 fatty acids. It is suggested that the increased LDL1 resulted from the utilization of VLDL triglyceride for milk fat formation.

Protected lipid feeding increased the proportion of C14:0, C16:0 C16:1 and C18:1 and decreased the proportions of C14:1 and C18:0 fatty acids in jugular serum triglycerides. Similar changes were observed in jugular VLDL triglycerides. Differences in the compositions of VLDL and LDL triglycerides across the mammary gland were observed and attributed either to selective uptake or to interchange of fatty acids between triglycerides and free fatty acids.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 94 , Issue 3 , June 1980 , pp. 503 - 516
Copyright
Copyright © Cambridge University Press 1980

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References

Banks, W., Clapperton, J. L. & Ferrie, M. E. (1976 a). Effect of feeding fat to dairy cows receiving a fafc-deficient basal diet. II. Fatty acid composition of the milk fat. Journal of Dairy Research 43, 219–227.CrossRefGoogle ScholarPubMed
Banks, W., Clapperton, J. L. & Ferrie, M. E. (1976 b). Physical properties of milk fats of different chemical compositions. Journal of the Society of Dairy Technology 29, 86.CrossRefGoogle Scholar
Bines, J. A., Brumby, P. E., Storry, J. E., Fulford, R. J. & Braithwaite, G. D. (1978). The effect of protected lipids on nutrient intakes, blood and rumen metabolites and milk secretion in dairy cows during early lactation. Journal of Agricultural Science, Cambridge 91, 135–150.CrossRefGoogle Scholar
British Standards Institution (1969). British Standard no. 696, part II, p. 7. London: British Standards Institution.Google Scholar
Brumby, P. E., Anderson, M. A., Tuckley, B., Storry, J. E. & Hibbitt, K. G. (1975). Lipid metabolism in the cow during starvation-induced ketosis. Biochemical Journal 146, 609–615.CrossRefGoogle ScholarPubMed
Brumby, P. E., Storry, J. E., Bines, J. A. & Fulford, R. J. (1977). Interrelationship between nutrient intakes, metabolite levels and milk secretion in dairy cows fed protected lipid during early lactation. Proceedings of the 12nd meeting of the American Dairy Science Association, p. 128.Google Scholar
Brumby, P. E., Storry, J. E., Bines, J. A. & Fulford, R. J. (1978). Utilization of energy for maintenance and production by dairy cows given protected tallow during early lactation. Journal of Agricultural Science, Cambridge 91, 151–159.CrossRefGoogle Scholar
Brumby, P. E., Storry, J. E. & Hall, A. J. (1972). Milk fat secretion at different stages of lactation. Report of the National Institute for Research in Dairying, p. 105.Google Scholar
Brumby, P. E., Storry, J. E. & Sutton, J. D. (1972). Metabolism of codliver oil in relation to milk fat secretion. Journal of Dairy Research 39, 167–182.CrossRefGoogle ScholarPubMed
Brumby, P. E., Storry, J. E., Sutton, J. D. & Oldham, J. D. (1979). Fatty acid digestion and utilisation by dairy cows. Annales de Recherche Vétérinaire 10, 317–319.Google Scholar
Brumby, P. E. & Welch, V. A. (1970). Fractionation of bovine serum lipoproteins and their characterization by gradient gel electrophoresis. Journal of Dairy Research 37, 121–128.CrossRefGoogle Scholar
Brumby, P. E. & Welch, V. A. (1978). Lipid precursors of milkfat. Biennial Reviews of the National Institute for Research in Dairying, pp. 39–67.Google Scholar
Dryden, F. D., Marchello, J. A., Cuitin, L. L. & Hale, W. H. (1975). Protein protected fat for ruminants. II. Serum lipids and lipoproteins. Journal of Animal Science 40, 697–705.Google Scholar
Dunkley, W. L., Smith, N. E. & Franke, A. A. (1977). Effects of feeding protected tallow on the composition of milk and milk fat. Journal of Dairy Science 60, 1863–1869.Google Scholar
Glascock, R. F. & Welch, V. A. (1974). Contributions of the fatty acids of three low density serum lipoproteins to bovine milk fat. Journal of Dairy Science 57, 1364–1370.CrossRefGoogle ScholarPubMed
Glascock, R. F. & Welch, V. A. (1978). Etude de l'incorporation des acides gras alimentaires dans les lipides du lait: influence de l'acide phytanique. Annales de Biologie Animate, Biochimie, Biophysique 18, 1443–1453.CrossRefGoogle Scholar
Goering, H. K., Gordon, C. H., Wrenn, T. R., Bitman, J., Kino, R. L. & Douglas, F. W. (1976). Effect of feeding protected safflower oil on yield, composition, flavour and oxidative stability of milk. Journal of Dairy Science 59, 416–425.Google Scholar
Gooden, J. M. & Lascelles, A. K. (1973). Effect of feeding protected lipid on the uptake of precursors of milk fat by the bovine mammary gland. Australian Journal of Biological Science 26, 1201–1210.CrossRefGoogle ScholarPubMed
Hartmann, P. E. & Lascelles, A. K. (1965). Variation in the concentration of lipids and some other constituents in the blood plasma of cows at various stages of lactation. Australian Journal of Biological Science 18, 114–123.CrossRefGoogle ScholarPubMed
Macleod, G. K., Yu, Y. & Schaeffer, L. R. (1977). Feeding value of protected animal tallow for high yielding dairy cows. Journal of Dairy Scierace 60, 726–738.CrossRefGoogle Scholar
Mentstky of Agriculture, Fishebies & Food (1975). Energy allowances and feeding systems for ruminants. Technical Bulletin no. 33.Google Scholar
Noble, R. C., Vernon, R. G., Christie, W. W., Moore, J. H. & Evans, A. J. (1977). The effects of dietary fats on the plasma lipid composition of sheep. Lipids 12, 423–433.Google Scholar
Palmquist, D. L., McClure, K. E. & Parker, C. F. (1977). Effect of protected saturated or polyunsaturated fat fed to pregnant and lactating ewes on milk composition, lamb plasma fatty acids and growth. Journal of Animal Science 45, 1152–1159.CrossRefGoogle ScholarPubMed
Patsch, J. R., Gotto, A. M., Olivecrona, T. & Eisenberg, S. (1978). Formation of high density Iipoprotein2-like particles during lipolysis of very low density lipoproteins in vitro. Proceedings of the National Academy of Sciences, U.S.A. 75, 4519–4523.CrossRefGoogle ScholarPubMed
Raphael, B. C., Dimick, P. S. & Puppione, D. L. (1973). Lipid characterisation of bovine serum lipoproteins throughout gestation and lactation. Journal of Dairy Science 56, 1025–1032.CrossRefGoogle ScholarPubMed
Smith, N. E., Dunkley, W. L. & Franke, A. A. (1978). Effects of feeding protected tallow to dairy cows in early lactation. Journal of Dairy Science 61, 747–756.Google Scholar
Stead, D. & Oldham, J. D. (1978). The effects of low-and high-protein diets upon the concentrations of plasma low density lipoproteins, in Friesian heifers throughout lactation. Proceedings of the Nutrition Society 3, A45.Google Scholar
Stead, D. & Welch, V. A. (1975). Lipid composition of bovine serum lipoproteins. Journal of Dairy Science 58, 122–127.Google Scholar
Stead, D. & Welch, V. A. (1976 a). Immunological properties of bovine serum lipoproteins and chemical analysis of their protein moieties. Journal of Dairy Science 59, 1–8.Google Scholar
Stead, D. & Welch, V. A. (1976 b). Determination of physical properties of bovine serum lipoproteins by analytical ultracentrifugation. Journal of Dairy Science 59, 9–13.Google Scholar
Storry, J. E. (1978). Manipulation of the synthesis and secretion of milk fat through nutrition of the dairy cow. Proceedings of the 20th International Dairy Congress, Lecture, 22pp.Google Scholar
Storry, J. E. (1980). The effect of nutrition on the yield and content of milk fat. 1. Effect of nonprotected fat. In Factors Affecting the Yields and Contents of Milk Constituents of Commercial Importance (ed. Moore, J. H. and Rook, J. A. F.) (in the Press).Google Scholar
Storry, J. E., Brumby, P. E. & Cheeseman, G. C. (1974). Protected lipids in ruminant nutrition. Agricultural Development Advisory Service Quarterly Review 15, 96–106.Google Scholar
Storby, J. E., Brumby, P. E. & Dunkley, W. L. (1980). The effect of nutrition on the yield and content of milk fat. 3. Effect of protected nonpolyunsaturated fats. In Factors Affecting the Yields and Contents of Milk Constituents of Commercial Importance (ed. Moore, J. H. and Rook, J. A. F.) (in the Press).Google Scholar
Storry, J. E., Brumby, P. E., Hall, A. J. & Johnson, V. W. (1974 a). Response in rumen fermentation and milk fat secretion in cows receiving low roughage diets supplemented with protected tallow. Journal of Dairy Research 41, 165–173.CrossRefGoogle Scholar
Storry, J. E., Bbumby, P. E., Hall, A. J. & Johnson, V. W. (1974 b). Response of the lactating cow to different methods of incorporating caseins and coconut oil in the diet. Journal of Dairy Science 57, 61–67.CrossRefGoogle ScholarPubMed
Storry, J. E., Brumby, P. E. & Tuckley, B. (1978). Effects of feeding protected tallow to dairy cows in early lactation on the yield and composition of milk fat. Proceedings of the 20th International Dairy Congress, p. 64.Google Scholar
Storry, J. E., Hall, A. J. & Johnson, V. W. (1973). The effects of increasing amounts of dietary tallow on milk fat secretion in the cow. Journal of Dairy Research 40, 293–299.CrossRefGoogle ScholarPubMed
Sutton, J. D., Storry, J. E. & Nicholson, J. W. G. (1970). The digestion of fatty acids in the stomach and intestines of sheep given widely different rations. Journal of Dairy Research 37, 97–105.Google Scholar
Tuckley, B. & Storry, J. E. (1974). An improved method for thin layer chromatography of plasma lipids by single development. Lipids 9, 493–494.CrossRefGoogle ScholarPubMed
Wilson, G. F. & Brookes, I. M. (1975). The influence of a protected tallow supplement on milk yield and composition. Proceedings of the New Zealand Society of Animal Production 35, 41–46.Google Scholar
Wrenn, T. R., Bitman, J., Waterman, R. A., Weyant, J. R., Wood, D. L., Strozinski, L. L. & Hooven, N. W. (1978). Feeding protected and unprotected tallow to lactating cows. Journal of Dairy Science 61, 49–58.CrossRefGoogle Scholar
Wrenn, T. R., Bitman, J., Weyant, J. R., Wood, D. L., Strozinski, L. L. & Hooven, N. W. (1976). Protected animal tallow fed to lactating cows. Journal of Animal Science 43, 339.Google Scholar
Yang, Y. T., Baldwin, R. L. & Russell, J. (1978). Effects of long supplementations with lipids on laotating dairy cows. Journal of Dairy Science 61, 180–188.Google Scholar
Yang, Y. T., Rohde, J. M. & Baldwin, R. L. (1978). Dietary lipid metabolism in lactating dairy cows. Journal of Dairy Science 61, 1400–1406.CrossRefGoogle ScholarPubMed