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The effect of diet on glucose entry rates in sheep

Published online by Cambridge University Press:  27 March 2009

M. J. Ulyatt
Affiliation:
Rowelt Research Institute, Bucksburn, Aberdeen, AB2 9SR
F. C. Whitelaw
Affiliation:
Rowelt Research Institute, Bucksburn, Aberdeen, AB2 9SR
F. G. Watson
Affiliation:
Rowelt Research Institute, Bucksburn, Aberdeen, AB2 9SR

Summary

An isotope dilution technique has been used to measure the rate of entry of glucose into the metabolic glucose pool and its rate of oxidation in three sheep given diets of barley (85% barley, 15% protein-mineral-vitamin supplement), dried grass or hay. A latin-square design was used and the sheep were fed continuously from an automatic feeding device.

Despite large differences in the amounts of α-linked glucose polymer and of crude protein supplied by the diets there were no significant differences between treatments in glucose entry rate, in plasma glucose concentration or in the size of the total body pool of glucose.

Calculations based on the specific radioactivity of expired CO2 after 3 h infusions indicated no significant differences between treatments in the percentage of glucose oxidised to CO2 or in the percentage of expired CO2 derived from glucose. The absence of a final ‘plateau’ position in the CO2 activity-time curve, even after 6 h infusions, casts doubt on the validity of these calculations.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1970

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References

Agricultural Research Council (1965). Nutrient Requirements of Farm Livestock no. 2, Ruminants. London: H.M.S.O.Google Scholar
Annison, E. F., Brown, R. E., Lend, R. A., Lindsay, D. B. & West, C. E. (1967). Rates of entry and oxidation of acetate, glucose, D(—)-β-hydroxybutyrate, palmitate, oleate and stearate, and rates of production and oxidation of propionate and butyrate in fed and starved sheep. Biochem. J. 104, 135.CrossRefGoogle ScholarPubMed
Annison, E. F. & White, R. R. (1961). Glucose utilization in sheep. Biochem. J. 80, 162.CrossRefGoogle Scholar
Armstrong, D. G. (1965). Carbohydrate metabolism in ruminants and energy supply. In Physiology of Digestion in the Ruminant (ed. Dougherty, R. W.), p. 272. Washington: Butterworth.Google Scholar
Balch, D. A. & Rowland, S. J. (1957). Volatile fatty acids and lactic acid in the rumen of dairy cows reoeiving a variety of diets. Br. J. Nutr. 11, 288.CrossRefGoogle ScholarPubMed
Bartley, J. C. & Black, A. L. (1966). Effect of exogenous glucose on glucose metabolism in dairy cows. J. Nutr. 89, 317.CrossRefGoogle ScholarPubMed
Bergman, E. N. (1963). Quantitative aspects of glucose metabolism in pregnant and non-pregnant sheep. Am. J. Physiol. 204, 147.CrossRefGoogle Scholar
Bergman, E. N., Roe, W. E. & Kon, K. (1966). Quantitative aspects of propionate metabolism and gluooneogenesis in sheep. Am. J. Physiol. 211, 793.CrossRefGoogle ScholarPubMed
Cluley, H. J. (1962). Suspension scintillation counting of carbon-14 barium carbonate. Analyst 87, 170.CrossRefGoogle Scholar
Eadie, J. M., Hyldgaard-Jensen, J., Mann, S. O., Reid, R. S. & Whitelaw, F. G. (1970). Observations on the microbiology and biochemistry of the rumen in cattle given different quantities of a pelleted barley ration. Br. J. Nutr. 24, 157.CrossRefGoogle ScholarPubMed
Ford, E. J. H. (1965). The effect of diet on glucose utilization by sheep. J. agric. Sci., Camb. 65, 41.CrossRefGoogle Scholar
Ford, E. J. H. & Reilly, P. E. B. (1969). The utilization of plasma-free amino acid and glucose carbon by sheep. Res. vet. Sci. 10, 409.CrossRefGoogle Scholar
Heald, P. J. (1952). The assessment of glucose containing substances in rumen micro-organisms during a digestion cyole in sheep. Br. J. Nutr. 5, 84.CrossRefGoogle Scholar
Huggett, A. St G. & Nixon, D. A. (1957). Use of glucose oxidase, peroxidase and o-dianisidine in determination of blood and urinary glucose. Lancet ii, 368.CrossRefGoogle Scholar
Jones, G. B. (1965). Determination of the specific activity of labelled blood glucose by liquid scintillation using glucose penta-acetate. Analyt. Biochem. 12, 249.CrossRefGoogle Scholar
Judson, G. J., Anderson, E., Luick, J. R. & Leng, R. A. (1968). The contribution of propionate to glucose synthesis in sheep given diets of different grain content. Br. J. Nutr, 22, 69.CrossRefGoogle Scholar
Kronfeld, D. S. & Simeson, M. G. (1961). Glucose biokinetics in sheep. Am. J. Physiol. 210, 639.CrossRefGoogle Scholar
Leng, R. A., Steel, J. W. & Luick, J. R. (1967). Contribution of propionate to glucose synthesis in sheep. Biochem. J. 103. 785.CrossRefGoogle ScholarPubMed
Lindsay, D. B. (1959). The significance of carbohydrate in ruminant metabolism. Vet. Rev. Annot. 5, 103.Google Scholar
MacRae, J. C. & Armstrong, D. G. (1966). Investigations of the passage of α-linked glucose polymers into the duodenum of the sheep. Proc. Nutr. Soc. 25, xxxiii.Google Scholar
MacRae, J. C. & Armstrong, D. G. (1968). Enzyme method for determination of α-linked glucose polymers in biological materials. J. Sci. Fd Agric. 19, 578.CrossRefGoogle Scholar
MacRae, J. C. & Armstrong, D. G. (1969). Studies on intestinal digestion in the sheep. 2. Digestion of some carbohydrates in hay, cereal and hay-cereal rations. Br. J. Nutr. 23, 377.CrossRefGoogle ScholarPubMed
Nikkila, E. A. & Ojala, J. (1964). Gluconeogenesis from glycerol in fasting rats. Life Sci. 3, 244.CrossRefGoogle Scholar
Ørskov, E. R., Fraser, C. & Kay, R. N. B. (1969). Dietary factors influencing the digestion of starch in the rumen and small and large intestine of early weaned lambs. Br. J. Nutr. 23, 217.CrossRefGoogle ScholarPubMed
Porter, P. & Singleton, A. G. (1965). The passage of carbohydrate from the stomach of a hay-fed goat. Biochem. J. 96, 59PGoogle Scholar
Robbins, J. D. & Bakke, J. E. (1967). Sheep and goat metabolism unit for the collection of excreta and expired C14O2. J. Anim. Sci. 26, 424.CrossRefGoogle Scholar
Steele, R., Wall, J. S.de Bodo, R. C. & Altszuler, N. (1956). Carbohydrate metabolism of hypophysectomized dogs as studied with radioactive glucose. Am. J. Physiol. 187, 15.CrossRefGoogle Scholar
Storry, J. E. & Millard, D. (1967). The determination of stem-volatile fatty acids in rumen liquor, blood plasma and milk fat. J. Sci. Fd Agric. 16, 417.CrossRefGoogle Scholar
Sutherland, T. M., Gupta, N. B., Reid, R. S. & Murray, M. G. (1963). Some effects of continuous feeding on digestion in the sheep. Proc. 6th Int. Congr. Nutr., Edinburgh, p. 579.Google Scholar
Topps, J. H., Kay, R. N. B. & Goodall, E. D. (1968). Digestion of concentrate and hay diets in the stomach and intestines of ruminants. I. Sheep. Br. J. Nutr. 22, 261.CrossRefGoogle ScholarPubMed
Tucker, R. E., Little, C. O., Mitchell, G. E., Hayes, B. W. & Karr, M. R. (1966). Starch digestion in different sections of the digestive tract of sheep. J. Anim. Sci. 25, 911.Google Scholar
Ulyatt, M. J., Whitelaw, F. G. & Watson, F. G. (1969). Glucose entry rates in sheep given diets of barley, dried grass or hay. Proc. Nutr. Soc. 28, 50 A.Google ScholarPubMed