Hostname: page-component-cd9895bd7-dk4vv Total loading time: 0 Render date: 2024-12-23T02:54:15.178Z Has data issue: false hasContentIssue false
  • English
  • Français

Diet-induced variation in acetate metabolism of ovine perirenal adipose tissue in vitro

Published online by Cambridge University Press:  27 March 2009

N. D. Scollan  and
N. S. Jessop
N. D. Scollan
Affiliation:
School of Agriculture, Institute of Ecology and Resource Management, The University of Edinburgh, The King's Buildings, West Mains Road, Edinburgh EH9 3JG, UK
N. S. Jessop
Affiliation:
School of Agriculture, Institute of Ecology and Resource Management, The University of Edinburgh, The King's Buildings, West Mains Road, Edinburgh EH9 3JG, UK
Get access Rights & Permissions [Opens in a new window]

Summary

Sixteen Blackface wether lambs (25–27 kg) were fed for 9 weeks on diets based on either barley (B) or sugarbeet pulp (S), with either a low or high protein content. A constant amount of metabolizable energy (ME) was given at twice maintenance level. At one stage the animals were fed frequently and blood was sampled over a 24 h period. Plasma was assayed for acetate, glucose and insulin. After slaughter, the metabolism of acetate to CO2 and lipid was investigated in perirenal adipocytes in vitro.

Frequent feeding helped to achieve near-steady-state conditions. Carbohydrate source influenced plasma acetate (P < 0·08) but not glucose or insulin. Level of protein in the diet influenced plasma acetate, higher concentrations of acetate were associated with lower levels of protein (P < 0·01). Acetate oxidation and incorporation into lipid was influenced by the concentrations of acetate, glucose and insulin in the incubation media (P < 0·001). Acetate oxidation was influenced by carbohydrate source (P < 0·05), whereas level of protein in the diet affected acetate incorporation into lipid (P < 0·05).

These results show how the balance of nutrients provided to a ruminant for metabolism may influence selected plasma parameters and the metabolism of acetate by adipose tissue, which may help to explain the reduced efficiency of ME utilization from more fibrous diets.

Type
Animals
Information
The Journal of Agricultural Science , Volume 125 , Issue 3 , December 1995 , pp. 429 - 436
Copyright
Copyright © Cambridge University Press 1995

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abdul-Razzaq, H. A. & Bickerstaffe, R. (1989). The influence of rumen volatile fatty acids on protein metabolism in growing lambs. British Journal of Nutrition 62, 297–310.CrossRefGoogle ScholarPubMed
Annison, E. F. & Armstrong, D. G. (1970). Volatile fatty acid metabolism and energy supply. In Physiology of Digestion and Metabolism in the Ruminant (Ed. Phillipson, A. T.), pp. 422–437. Newcastle: Oriel Press.Google Scholar
Bassett, J. M., Weston, R. H. & Hogan, J. P. (1971). Dietary regulation of plasma insulin and growth hormone concentrations in sheep. Australian Journal of Biological Sciences 24, 321–330.CrossRefGoogle ScholarPubMed
Blaxter, K. L. (1962). The Energy Metabolism of Ruminants. London: Hutchinson Scientific and Technical.Google Scholar
Bryant, D. T. W. & Smith, R. W. (1982). Protein synthesis in muscle of mature sheep. Journal of Agricultural Science, Cambridge 98, 639–643.CrossRefGoogle Scholar
Etherton, T. D. & Evock, C. M. (1986). Stimulation of lipogenesis in bovine adipose tissue by insulin and insulin-like growth factor. Journal of Animal Science 62, 357–362.CrossRefGoogle ScholarPubMed
Etherton, T. D., Chung, C. S. & Wiggins, J. P. (1984). Receptor-dependent and independent degradation of insulin by isolated swine adipocytes at 37 °C. Journal of Animal Science 59, 366–375.CrossRefGoogle ScholarPubMed
Folch, J., Lees, M. & Sloane Stanley, G. H. (1957). A simple method for the isolation and purification of total lipides from adipose tissues. Journal of Biological Chemistry 226, 497–509.CrossRefGoogle Scholar
Galbraith, H., McCulloch, T., Scaife, J. R., Keeling, B. J. & Atkinson, T. (1988). Effect of barley, sugar-beet pulp and protein level in the diet on growth, body composition and blood metabolites and insulin concentrations of entire male lambs. Animal Production 46, 514 (Abstract 104).Google Scholar
Giesecke, D. (1983). The pools of cellular nutrients: plasma free fatty acids. In Dynamic Biochemistry of Animal Production (Ed. Riis, P. M.), pp. 197–214. World Animal Science, A3. Amsterdam: Elsevier Science Publishers.Google Scholar
Girdler, C. P., Thomas, P. C. & Chamberlain, D. G. (1986). Exogenous supply of glucose precursors and nitrogen utilization in sheep. Proceedings of the Nutrition Society 45, 43A.Google Scholar
Guynn, R. W. & Veech, R. L. (1974). Direct enzymic determination of acetate in tissue extracts in the presence of labile acetate esters. Analytical Biochemistry 61, 6–15.Google Scholar
Ketelaars, J. J. M. H. & Tolkamp, B. J. (1992). Toward a new theory of feed intake regulation in ruminants. 3. Optimum feed intake: in search of a physiological background. Livestock Production Science, 31, 235–258.CrossRefGoogle Scholar
Leng, R. A., Jessop, N. S. & Kanjanapruthipong, J. (1993). Control of feed intake and the efficiency of utilisation of feed by ruminants. In Recent Advances in Animal Nutrition in Australia (Ed. Farrell, D. J.), p. 70–88. Armidale: The University of New England Publishing Unit.Google Scholar
Lobley, G. E. & MacRae, J. C. (1987). Acetate utilization in sheep. In Energy Metabolism of Farm Animals (Eds Moe, P. W., Tyrrell, H. F. & Reynolds, P. J.), pp. 38–41. Proceedings of the 10th Symposium, EAAP Publication No. 32. Totowa, NJ: Rowman & Littlefield.Google Scholar
MacRae, J. C. & Lobley, G. E. (1982). Some factors which influence thermal energy losses during the metabolism of ruminants. Livestock Production Science 9, 447–456.CrossRefGoogle Scholar
MacRae, J. C. & Lobley, G. E. (1986). Interactions between energy and protein. In Control of Digestion and Metabolism in Ruminants (Eds Milligan, L. P., Grovum, W. L. & Dobson, A.), pp. 367–385. Englewood Cliffs, NJ: Prentice-Hall.Google Scholar
Preston, T. R. & Leng, R. A. (1987). Matching Ruminant Production Systems with Available Resources in the Tropics and Sub-Tropics. Armidale: Penambul Books.Google Scholar
Prior, R. L. & Smith, S. B. (1982). Hormonal effects on partitioning of nutrients for tissue growth: role of insulin. Federation Proceedings 41, 2545–2549.Google Scholar
Scollan, N. D. (1990). Biochemical aspects of energy utilisation in ruminants. PhD thesis, University of Edinburgh.Google Scholar
Scollan, N. D. & Jessop, N. S. (1991). Effect of carbohydrate source and protein level on selected plasma characteristics and acetate metabolism of ovine perirenal adipose tissue in vitro. Proceedings of the Nutrition Society 50, 77A.Google Scholar
Trenkle, A. (1978). Relation of hormonal variations to nutritional studies and metabolism of ruminants. Journal of Dairy Science 61, 281–293.Google Scholar
Tyrrell, H. F., Reynolds, P. J. & Moe, P. W. (1979). Effect of diet on partial efficiency of acetate use for body tissue synthesis by mature cattle. Journal of Animal Science 48, 598–606.Google Scholar
Vernon, R. G. (1979). Metabolism of ovine adipose tissue in tissue culture. International Journal of Biochemistry 10, 57–60.Google Scholar
Waghorn, G. C., Flux, D. S. & Ulyatt, M. J. (1987). Effects of dietary protein and energy intakes on growth hormone, insulin, glucose tolerance and fatty acid synthesis in young wether sheep. Animal Production 44, 143–152.Google Scholar
Walsh, A., Gill, M. & Beever, D. E. (1990). The effect of fish meal supplementation on in-vitro subcutaneous adipose tissue metabolism in young cattle fed silage. Animal Production 50, 584 (Abstract 134).Google Scholar
Walton, P. E., Etherton, T. D. & Rebhun, J. F. (1984). Stimulatory effects of insulin on glucose metabolism in swine adipose tissue. Journal of Animal Science 59 (Supplement 1), 211 (Abstract 161).Google Scholar
Weekes, T. E. C. (1986). Insulin and growth. In Control and Manipulation of Animal Growth (Eds Buttery, P. J., Haynes, N. B. & Lindsay, D. B.), pp. 187–206. London: Butterworths.CrossRefGoogle Scholar
Yang, Y. T. & Baldwin, R. L. (1973). Preparation and metabolism of isolated cells from bovine adipose tissue. Journal of Dairy Science 56, 350–365.Google Scholar