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The concentration of soluble polysaccharides in the rumen contents of sheep fed on hay

Published online by Cambridge University Press:  27 March 2009

P. N. Hobson  and
J. K. Thompson
P. N. Hobson
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen
J. K. Thompson
Affiliation:
School of Agriculture, Aberdeen
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Summary

On the feeding regimes examined, i.e. hay ad libitum restricted hay and restricted hay with supplementary glucose, the quantities of extracellular water-soluble polysaccharides in rumen liquor were small (3·6–9·0 mg/100 ml) and could have originated from the diet or the saliva as well as from microbes. The polysaccharides extracted with hot water (fraction B) and acetate buffer (fraction C) were also small in amount in the rumen liquor from sheep on any of these diets (less than 56 mg/100 ml) and were composed of rhamnose—containing polysaccharides, starch-like polysaccharides and xylose polymers. Syntheses of these B and C polysaccharides were found with the hay and glucose diet but the quantities involved were small (12·27 mg/100 ml rumen liquor). Degradation of the polysaccharides occurred at 4–6 h after feeding. The consistently high ratios of rhamnose to hexose in the B and C polysaccharides from rumen liquor suggest that these were microbial in origin. Iodine-staining organisms in the rumen liquor were a small proportion of the total and showed no increases after feeding.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 75 , Issue 3 , December 1970 , pp. 471 - 478
Copyright
Copyright © Cambridge University Press 1970

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References

Bailey, R. W. (1959). Bloat in cattle. XIV. The concentration of soluble polysaccharide in the rumen contents of cows fed on red clover. N.Z. Jl agric. Res. 2, 355–65.CrossRefGoogle Scholar
Dische, Z. (1962). Color reactions of pentoses. In Methods in Carbohydrate Chemistry 1 (Ed. Whistler, R. L. and Wolfram, M. L.). New York: Academic Press.Google Scholar
Gibbons, M. N. (1955). The determination of methylpentoses. Analyst., Lond. 80, 268–76.Google Scholar
Gurr, E. (1958). Methods of Analytical Histology and Histo-Chemistry. London: Leonard Hill.Google Scholar
Heald, P. J. (1951). The assessment of glucose containing substances in rumen micro-organisms, during a digestion cycle in sheep. Br. J. Nutr. 5, 84–93.Google Scholar
Hobson, P. N. & MacPherson, M. J. (1954). Some serological and chemical studies on materials extracted from an amylolytic streptococcus from the rumen of the sheep. Biochem. J. 57, 145–51.CrossRefGoogle ScholarPubMed
Hough, L. & Jones, J. K. N. (1962). Chromatography on paper. In Methods in Carbohydrate Chemistry 1 (Ed. Whistler, R. L. and Wolfram, M. L.). New York: Academic Press.Google Scholar
Hydén, S. (1955). A turbidimetric method for the determination of higher polyethylene glycols in biological materials. K. Lantbrhögsk. Annlr 22, 139–45.Google Scholar
Hydén, S. (1961). Determinations of the amount of fluid in the reticulo-rumen of the sheep and its rate of passage to the omasum. K. Lantbrhögsk. Annlr 27, 51–79.Google Scholar
Jacobson, D. R., Lindahl, I. L., MacNeil, J. J., Shaw, J. C., Doetsoh, R. N. & Davis, R. E. (1957). Feedlot bloat studies. II. Physical factors involved in the etiology of frothy bloat. J. Anim. Sci. 16, 515–23.CrossRefGoogle Scholar
McDougall, E. I. (1948). Studies on ruminant saliva. 1. The composition and output of sheep's saliva. Biochem. J. 43, 99–109.Google Scholar
MacRae, J. C. & Armstrong, D. G. (1969). Studies on intestinal digestion in sheep. 2. Digestion of some carbohydrate constituents in hay, cereal and haycereal rations. Br. J. Nutr. 23, 377–87.CrossRefGoogle ScholarPubMed
Nicholson, J. W. G. & Sutton, J. D. (1969). The effect of diet composition and level of feeding on digestion in the stomach and intestines of sheep. Br. J. Nutr. 23, 585–601.CrossRefGoogle ScholarPubMed
Ørskov, E. R., Fraser, C. & Kay, R. N. B. (1969). Dietary factors influencing the digestion of starch in the rumen and small and large intestine of early weaned lambs. Br. J. Nutr. 23, 217–26.CrossRefGoogle ScholarPubMed
Porter, P. & Singleton, A. G. (1966). The digestion of starch and fibre polysaccharide in sheep fed hay and concentrates. J. Physiol., Lond. 186, 145P.Google Scholar
Topps, J. H., Kay, R. N. B. & Goodall, E. D. (1968). Digestion of concentrate and hay diets in the stomach and intestines of ruminants. 1. Sheep. Br. J. Nutr. 22, 261–80.Google Scholar
Trevelyan, W. E. & Harrison, J. S. (1952). Studies on yeast metabolism. 1. Fractionation and microdetermination of cell carbohydrates. Biochem. J. 50, 298–309.CrossRefGoogle Scholar
Tucker, R. E., Mitchell, G. E. & Little, C. O. (1968). Ruminal and postruminal starch digestion in sheep. J. Anim. Sci. 27, 824–6.CrossRefGoogle ScholarPubMed
Weller, R. A. & Gray, F. V. (1954). The passage of starch through the stomach of the sheep. J. exp. Biol. 31, 40–8.CrossRefGoogle Scholar
Wright, P. L., Grainger, R. B. & Marco, G. J. (1966). Post-ruminal degradation and absorption of carbohydrate by the mature ruminant. J. Nutr. 89, 241–6.Google Scholar
Yemm, E. W. & Willis, A. J. (1954). The estimation of carbohydrates in plant extracts by anthrone. Biochem. J. 57, 508–14.CrossRefGoogle ScholarPubMed