Published online by Cambridge University Press: 27 March 2009
Seed extracts prepared by soaking ryegrass seed in water were tested for their ability to suppress germination. The tests were carried out by sowing seed on to sand-filled basins moistened with seed extract.
Extracts prepared from the two diploid varieties, S. 24 and Hora and the tetraploid variety Terhoy were able to reduce both the rate of germination and the final percentage germination when applied to the sown seed. The details were as follows, (i) When S. 24 perennial ryegrass seed was used to obtain the extract inhibition was found to be related to the concentration of the extract, (ii) The final germination of diploid seed was reduced by 16% in the presence of its own seed extract and by 27% when tetraploid seed extract was applied, (iii) The final germination percentage of the tetraploid seed was reduced by 14% in the presence of either its own extract or that prepared from the diploid seed, (iv) Simple chemical tests carried out on the seed extract, although not conclusive, gave some indication that the inhibitory substance may have been a member of the alkaloid group.