1. Italian ryegrass, laid down for two years, was used to compare the value of casein and formalized casein, ammonium sulphate, calcium nitrate and urea for grassland production. Residual effects of these treatments were measured on a third-year crop of barley.

2. Single dressings of ammonium sulphate and casein were of similar value at each of three cuttings in 1956.

3. Casein treated with formalin released nitrogen much more slowly and was of little value in the early part of the growing season. Formalized casein produced significantly higher yields than casein at the later cuttings in both years, but aggregate increases from this material were much lower than those obtained from untreated casein in each season. Formalized casein increased yields considerably in the year following application.

4. Single dressings of formalized casein were also compared with ‘repeated’ dressings of ammonium sulphate, calcium nitrate and urea, in which the same total quantity of nitrogen was divided equally between cuttings. Formalized casein produced much lower yields than these ‘repeated’ dressings in the early part of the season, but was of only slightly less value at the last cut in 1956 and gave the highest yield at the final cut in 1957. Aggregate yields from repeated dressings of inorganic N fertilizers were higher than those given by single dressings of either form of casein.

5. Comparisons were also made between the yields given by ammonium sulphate, calcium nitrate and urea. Urea applied before sowing (at 0·5 cwt. N/acre) damaged germination and reduced plant establishment slightly. Under dry conditions both urea and calcium nitrate tended to give higher yields than ammonium sulphate but with adequate rain all three fertilizers gave similar yields.