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Chitosan and microbial inoculants in whole-plant soybean silage

Published online by Cambridge University Press:  29 June 2021

J. P. G. de Morais ,
R. Cantoia Júnior ,
T. M. Garcia ,
E. Capucho ,
M. Campana ,
J. R. Gandra ,
Lucas G. Ghizzi  and
T. A. Del Valle Open the ORCID record for T. A. Del Valle [Opens in a new window]
J. P. G. de Morais
Affiliation:
Department of Biotechnology Vegetal and Animal Production, Agricultural Science Center, Federal University of São Carlos, Araras, 13600-970, Brazil
R. Cantoia Júnior
Affiliation:
Umuarama Campus, State University of Maringá, Umuarama, PR 87506-370, Brazil
T. M. Garcia
Affiliation:
Department of Biotechnology Vegetal and Animal Production, Agricultural Science Center, Federal University of São Carlos, Araras, 13600-970, Brazil
E. Capucho
Affiliation:
Department of Biotechnology Vegetal and Animal Production, Agricultural Science Center, Federal University of São Carlos, Araras, 13600-970, Brazil
M. Campana
Affiliation:
Department of Biotechnology Vegetal and Animal Production, Agricultural Science Center, Federal University of São Carlos, Araras, 13600-970, Brazil
J. R. Gandra
Affiliation:
Institute of Agrarian and Regional Development, Federal University of Southern and Southeastern Pará, Marabá, 68.555-410, Brazil
Lucas G. Ghizzi
Affiliation:
Department of Animal Nutrition and Production, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, 13.635-900, Brazil
T. A. Del Valle*
Affiliation:
Departament of Animal Science, Rural Sciences Center, Federal University of Santa Maria, Santa Maria, RS, 97105-340, Brazil.
*
Author for correspondence: T. A. Del Valle, E-mail: [email protected]
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Abstract

Whole-plant soybean silage (WPSS) is a potential high-protein roughage source for ruminant diets. However, WPSS can be difficult to ensile and fermentation is a challenge. This study was conducted to evaluate the effect of chitosan and microbial inoculants on fermentation profile, fermentation losses, chemical composition, and in vitro degradation of WPSS. Forty experimental silos (PVC tubing with 28 cm i.d. and 25 cm height) were produced. Soybean plants from 10 plots were ensiled in a completely randomized block design to evaluate the following treatments: (1) control (CON): WPSS without additives; (2) chitosan (CHI): WPSS additive with 6 g/kg DM of chitosan; (3) LBB: WPSS treated with 5.0 × 107 colony-forming units (CFU) of Lactobacillus buchneri (NCIM 40788) per kg of fresh matter and (4) LPP: WPSS treated with 1.6 × 108 CFU of Lactobacillus plantarum and 1.6 × 108 CFU of Pediococcus acidilactici per kg of fresh matter. Silos were opened 120 days after ensiling. Microbial inoculants reduced silage pH, whereas LPP-treated silos showed the lowest concentration of NH3-N, ethanol, butyric, acetic, branched-chain, and propionic organic acids. LBB-treatment decreased lactic acid bacteria (LAB) count relative to other treatments, and LPP-treatment showed the lowest fermentation losses, improving dry matter (DM) recovery. Relative to other treatments, LPP increased silage DM, organic matter, and decreased acid detergent insoluble crude protein (CP), improving DM and neutral detergent fibre in vitro degradation. Treatments showed no effect on silage aerobic stability. Thus, LPP-treatment improves fermentation profile, reduces fermentation losses, and increases the nutritional value of WPSS.

Keywords

Chitin derivatedigestibilityL. buchneriLegume silage
Type
Crops and Soils Research Paper
Information
The Journal of Agricultural Science , Volume 159 , Issue 3-4 , April 2021 , pp. 227 - 235
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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