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BANYULS genes from Brassica juncea and Brassica nigra: cloning, evolution and involvement in seed coat colour

Published online by Cambridge University Press:  14 July 2016

L. L. LIU ,
T. HUANG ,
S. P. DING ,
Y. WANG  and
M. L. YAN
L. L. LIU
Affiliation:
School of Life Science, Hunan University of Science and Technology, Xiangtan, Hunan 411201, China Key Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-Polluted Soils, Hunan Provincial Education Department, Xiangtan, Hunan 411201, China
T. HUANG
Affiliation:
School of Life Science, Hunan University of Science and Technology, Xiangtan, Hunan 411201, China Key Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-Polluted Soils, Hunan Provincial Education Department, Xiangtan, Hunan 411201, China
S. P. DING
Affiliation:
School of Life Science, Hunan University of Science and Technology, Xiangtan, Hunan 411201, China Key Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-Polluted Soils, Hunan Provincial Education Department, Xiangtan, Hunan 411201, China
Y. WANG
Affiliation:
School of Life Science, Hunan University of Science and Technology, Xiangtan, Hunan 411201, China Key Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-Polluted Soils, Hunan Provincial Education Department, Xiangtan, Hunan 411201, China
M. L. YAN*
Affiliation:
School of Life Science, Hunan University of Science and Technology, Xiangtan, Hunan 411201, China Key Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-Polluted Soils, Hunan Provincial Education Department, Xiangtan, Hunan 411201, China
*
*To whom all correspondence should be addressed. Email: [email protected]
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Summary

The molecular mechanism underlying the yellow seed trait has been a subject of quality breeding in Brassica. Thus, uncovering the biosynthetic pathway of proanthocyanidin (PA) accumulation in the Brassica seed coat is a promising research programme. Arabidopsis thaliana BANYULS (BAN) encodes anthocyanidin reductase, which is involved in seed coat pigmentation. In the current study, 2 and 4 BAN homologues were isolated using one pair of primers from Brassica nigra and Brassica juncea, respectively. Reverse transcription polymerase chain reaction (PCR) analysis showed that BAN was expressed abundantly in the seed coat of black seeds and in the embryos of all lines, but not in the seed coat of yellow seeds. Primers incorporating B genome-specific nucleotide variations were designed according to previously published BAN gene sequences of Brassica species to discern the BAN sequence located in B genome origin of Brassica using allele-specific PCR amplification. Proanthocyanidins were also detected by p-dimethylaminocinnamaldehyde staining and a butanol–hydrochloric acid (BuOH–HCl) colorimetric assay in the seed coat of black seeds, but not in the seed coat of yellow seeds. Anthocyanins were not also detected in the seed coat of Brassica species by the BuOH–HCl assay. Both transcriptional and chemical analyses suggested that BAN genes could be involved in both the biosynthesis of PAs and colour formation in the seed coat of Brassica species, whereas no expression of the BAN gene could block biosynthesis of PAs in the yellow seed coat.

Type
Crops and Soils Research Papers
Information
The Journal of Agricultural Science , Volume 155 , Issue 3 , April 2017 , pp. 421 - 430
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Copyright
Copyright © Cambridge University Press 2016 

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References

REFERENCES

Auger, B., Baron, C., Lucas, M. O., Vautrin, S., Bergès, H., Chalhoub, B., Fautrel, A., Renard, M. & Nesi, N. (2009). Brassica orthologues from BANYULS belong to a small multigene family, which is involved in procyanidin accumulation in the seed. Planta 230, 1167–1183.Google Scholar
Chai, Y. R., Lei, B., Huang, H. L., Li, J. N., Yin, J. M., Tang, Z. L., Wang, R. & Chen, L. (2009). TRANSPARENT TESTA 12 genes from Brassica napus and parental species, cloning, evolution, and differential involvement in yellow seed trait. Molecular Genetics and Genomics 81, 109–123.Google Scholar
Cheng, F., Liu, S. Y., Wu, J., Fang, L., Sun, S. L., Liu, B., Li, P. X., Hua, W. & Wang, X. W. (2011). BRAD, the genetics and genomics database for Brassica plants. BMC Plant Biology 11, 136. doi: 10.1186/1471-2229-11-136 Google Scholar
Cui, C., Ge, X., Gautam, M., Kang, L. & Li, Z. (2012). Cytoplasmic and genomic effects on meiotic pairing in Brassica hybrids and allotetraploids from pair crosses of three cultivated diploids. Genetics 191, 725–738.Google Scholar
Debeaujon, I., Nesi, N., Perez, P., Devic, M., Grandjean, O., Caboche, M. & Lepiniec, L. (2003). Proanthocyanidin-accumulating cells in Arabidopsis testa, regulation of differentiation and role in seed development. Plant Cell 15, 2514–2531.Google Scholar
Dixon, R. A., Xie, D. Y. & Sharma, S. B. (2005). Proanthocyanidins – a final frontier in flavonoid research? New Phytologist 165, 9–28.Google Scholar
Gesell, A., Yoshida, K., Tran, L. T. & Constabel, C. P. (2014). Characterization of an apple TT2-type R2R3 MYB transcription factor functionally similar to the poplar proanthocyanidin regulator PtMYB134. Planta 240, 497–511.CrossRefGoogle Scholar
Heneen, W. K., Geleta, M., Brismar, K., Xiong, Z., Pires, J. C., Hasterok, R., Stoute, A. I., Scott, R. J., King, G. J. & Kurup, S. (2012). Seed colour loci, homoeology and linkage groups of the C genome chromosomes revealed in Brassica rapa-B. oleracea monosomic alien addition lines. Annals of Botany 109, 1227–1242.Google Scholar
Howell, E. C., Kearsey, M. J., Jones, G. H., King, G. J. & Armstrong, S. J. (2008). A and C genome distinction and chromosome identification in Brassica napus by sequential fluorescence in situ hybridization and genomic in situ hybridization. Genetics 180, 1849–1857.Google Scholar
Lepiniec, L., Debeaujon, I., Routaboul, J. M., Baudry, A., Pourcel, L., Nesi, N. & Caboche, M. (2006). Genetics and biochemistry of seed flavonoids. Annual Review of Plant Biology 57, 405–430.Google Scholar
Li, X., Chen, L., Hong, M. Y., Zhang, Y., Zu, F., Wen, J., Yi, B., Ma, C. Z., Shen, J., Tu, J. X. & Fu, T. D. (2012). A large insertion in bHLH transcription factor BrTT8 resulting in yellow seed coat in Brassica rapa . PLoS ONE 7, e44145. doi: 10.1371/journal.pone.0044145 Google Scholar
Li, Y. G., Tanner, G. & Larkin, P. (1996). The DMACA-HCl protocol and the threshold proanthocyanidin content for bloat safety in forage legumes. Journal of the Science of Food and Agriculture 70, 89–101.Google Scholar
Lu, Y., Liu, X. J., Liu, S. Y., Yue, Y. C., Guan, C. Y. & Liu, Z. S. (2012). A simple and rapid procedure for identification of seed coat colour at the early developmental stage of Brassica juncea and Brassica napus seeds. Plant Breeding 131, 176–179.Google Scholar
Marles, M. A. S. & Gruber, M. Y. (2004). Histochemical characterisation of unextractable seed coat pigments and quantification of extractable lignin in the Brassicaceae. Journal of the Science of Food and Agriculture 84, 251–262.Google Scholar
Marles, M. A. S., Gruber, M. Y., Scoles, G. J. & Muir, A. D. (2003). Pigmentationin the developing seed coat and seedling leaves of Brassica carinata is controlled at the dihydroflavonol reductase locus. Phytochemistry 62, 663–672.Google Scholar
Nesi, N., Lucas, M. O., Auger, B., Baron, C., Lécureuil, A., Guerche, P., Kronenberger, J., Lepiniec, L., Debeaujon, I. & Renard, M. (2009). The promoter of the Arabidopsis thaliana BAN gene is active in proanthocyanidin-accumulating cells of the Brassica napus seed coat. Plant Cell Reports 28, 601–617.Google Scholar
Padmaja, L. K., Agarwal, P., Gupta, V., Mukhopadhyay, A., Sodhi, Y. S., Pental, D. & Pradhan, A. K. (2014). Natural mutations in two homoeologous TT8 genes control yellow seed coat trait in allotetraploid Brassica juncea (AABB). Theoretical and Applied Genetics 127, 339–347.Google Scholar
Parkin, I. A. P., Lydiate, D. J. & Trick, M. (2002). Assessing the level of collinearity between Arabidopsis thaliana and Brassica napus for A. thaliana chromosome 5. Genome 45, 356–366.Google Scholar
Parkin, I. A. P., Gulden, S. M., Sharpe, A. G., Lukens, L., Trick, M., Osborn, T. C. & Lydiate, D. J. (2005). Segmental structure of the Brassica napus genome based on comparative analysis with Arabidopsis thaliana . Genetics 171, 765–781.Google Scholar
Sharma, S. B. & Dixon, R. A. (2005). Metabolic engineering of proanthocyanidins by ectopic expression of transcription factors in Arabidopsis thaliana . Plant Journal 44, 62–75.Google Scholar
Wei, Q. (2010). Laboratory Manual for Molecular Biology, 2rd edn, Beijing: Higher Education Press.Google Scholar
Wu, Y. H., Xiao, L., Wu, G. & Lu, C. M. (2007). Cloning of fatty acid elongase 1 gene and molecular identification of A and C genome in Brassica species. Science in China Series C: Life Sciences 50, 343–349.Google Scholar
Xie, D. Y., Sharma, S. B., Paiva, N. L., Ferreira, D. & Dixon, R. A. (2003). Role of anthocyanidin reductase, encoded by BANYULS in plant flavonoid biosynthesis. Science 299, 396–399.Google Scholar
Yan, M. L., Liu, X. J., Liu, Z. S., Guan, C. Y., Yuan, M. Z. & Xiong, X. H. (2008). Cloning and expression analysis of dihydroflavonol 4-reductase gene in Brassica juncea . Acta Agronomica Sinica 34, 1–7.Google Scholar
Yan, M. L., Liu, Z. S., Guan, C. Y., Chen, S. Y., Yuan, M. Z. & Liu, X. J. (2009). Inheritance and molecular markers for the seed coat color in Brassica juncea . Frontiers of Agriculture in China 3, 1–6.Google Scholar
Yan, M. L., Liu, X. J., Guan, C. Y., Liu, L. L., Lu, Y. & Liu, Z. S. (2010). Cloning and SNP analysis of TT1 gene in Brassica juncea . Acta Agronomica Sinica 36, 1634–1641.Google Scholar
Yan, M. L., Liu, X. J., Guan, C. Y., Chen, X. B. & Liu, Z. S. (2011). Cloning and expression analysis of an anthocyanidin synthase gene homologue from Brassica juncea . Molecular Breeding 28, 313–322.Google Scholar
Yan, M. L., Liu, L. L., Wang, S. B. & Yao, Y. Y. (2013). A suitable cryo-sectioning method for visualizing proanthocyanidins in plant tissues by light microscopy. Plant Physiology Journal 49, 829–831.Google Scholar
Zhang, J. F., Lu, Y., Yuan, Y. X., Zhang, X. W., Geng, J. F., Chen, Y., Cloutier, S., McVetty, P. B. E. & Li, G. Y. (2009). Map-based cloning and characterization of a gene controlling hairiness and seed coat color traits in Brassica rapa . Plant Molecular Biology 69, 553–563.Google Scholar
Zhu, Y., Peng, Q. Z., Li, K. G. & Xie, D. Y. (2014). Molecular cloning and functional characterization of the anthocyanidin reductase gene from Vitisbellula . Planta 240, 381–398.Google Scholar