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An approach to the determination of rumen degradability of nitrogen in low-grade roughages and partition of nitrogen therein

Published online by Cambridge University Press:  27 March 2009

S. S. Negi ,
B. Singh  and
H. P. S. Makkar
S. S. Negi
Affiliation:
Indian Veterinary Research InstituteRegional Station, Palampur, H.P. 176061, India
B. Singh
Affiliation:
Indian Veterinary Research InstituteRegional Station, Palampur, H.P. 176061, India
H. P. S. Makkar
Affiliation:
Indian Veterinary Research InstituteRegional Station, Palampur, H.P. 176061, India
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Summary

Rumen degradability of dry matter (D.M.) and N in wheat straw, rice straw and local grass hay, containing 5·16, 6·37 and 4·58 mg N/g D.M. respectively, was estimated by rumen fermentation measurements at 3, 6, 9, 12, 24 and 48 h using the nylon bag technique. The degradability of D.M. at an assumed rumen outflow rate of 0·025/h was 21·7, 34·9 and 22·4 for wheat straw, rice straw and grass hay, respectively. Theestimation of degradability of N was confounded by thedeposition of ruminal microbial nitrogen in the nylon bag. A correction was made by measuring the accumulation of N when N·free fibre and cellulose were incubated in the nylon bags. The effective degradability of N in the roughages at the assumed rumen outflow rate was 54·5, 36·9 and 42·8% respectively, showing that the roughages contain rumen-degradable N for microbial nutrition. The undegradable nitrogen (UDN) values in wheat straw, rice straw and grass hay were 2·35, 4·02 and 2·62 mg/g D.M., and after correction for unavailable acid detergent-linked N, the available UDN values were 0·58, 2·95 and 1·32 mg/g D.M., respectively.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 111 , Issue 3 , December 1988 , pp. 487 - 494
Copyright
Copyright © Cambridge University Press 1988

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References

Agricultural Research Council (1980). The Nutrient Requirements of Ruminant Livestock. Farnham Royal, Slough: Commonwealth Agricultural Bureaux.Google Scholar
Armentano, L. E., Herrigton, T. A., Polan, C. E., Moe, A. J., Herbein, J. H. & Umstadt, P. (1986). Rumen degradation of dried Brewers grains, wet Brewers grains and soyabean meal. Journal of Dairy Science 69, 2124–2133.CrossRefGoogle Scholar
Association of Official Analytical Chemists (1970). Official Methods of Analysis of the Association of Official Analytical Chemists, 11th edn.Washington, D.C.: A.O.A.C.Google Scholar
Balyea, R. L., Darey, B. K. & Jones, K. S. (1979). Rate and extent of digestion and potentially digestible cell wall of waste paper. Journal of Animal Science 49, 887–892.CrossRefGoogle Scholar
Barrio, J. R., Owen, F. N. & Goetsen, A. L. (1985). Soluble nutrients in protein supplements and in situ disappearance. Canadian Journal of Animal Science 65, 667–672.CrossRefGoogle Scholar
De Faria, V. P. & Huber, J. T. (1984). Influence of dietary protein and energy on disappearance of dry matter from different forage types from dacron bags suspended in the rumen. Journal of Animal Science 59, 246–252.CrossRefGoogle Scholar
Filmer, D. G. (1982). Forage Protein in Ruminant Animal Production (ed. Thompson, D. J., Beever, D. E. and Guna, R. G.), pp. 129–138. British Society of Animal Production, Occasional Publication No. 6.Google Scholar
Freer, M. & Dove, H. (1984). Rumen degradation of protein in sunflower meal, rapeseed meal and lupin seed placed in nylon bags. Animal Feed Science and Technology 11, 87–107.CrossRefGoogle Scholar
Goering, H. K. & Van Soest, P. J. (1970). Forage Fiber Analysis. USDA Agricultural Handbook No. 379, Washington, D.C.Google Scholar
Ha, J. K. & Kennelly, J. J. (1984). In situ dry matter and protein degradation of various protein sources in dairy cattle. Canadian Journal of Animal Science 64, 443–452.CrossRefGoogle Scholar
Kennedy, P. M., Hazelwood, G. P. & Milligan, L. P. (1984). A comparison of methods for the estimation of the proportion of microbial nitrogen in duodenal digesta and of correction for microbial contamination in nylon bags incubated in rumen of sheep. British Journal of Nutrition 52, 403–417.CrossRefGoogle ScholarPubMed
Krishnamoorthy, U., Sniffen, C. J. & Van Soest, P. J. (1982). Nitrogen fractionation in ruminant feedstuffs for feed evaluation. Proceedings, Cornell Nutrition Conference for Feed Manufacturers, pp. 95–102.Google Scholar
Krishnamoorthy, U., Sniffen, C. J., Stern, M. D. & Van Soest, P. J. (1983). Evaluation of a mathematical model for rumen digestion and an in vitro simulation of rumen proteolysis to estimate the rumen undegraded nitrogen content of feedstuffs. Journal of Nutrition 50, 555–568.CrossRefGoogle Scholar
Leibholz, J. & Kellaway, R. C. (1984). The Role of Nitrogen and Energy Supplements. Sydney, N.S.W.: Australian Meat Research Committee.Google Scholar
Merry, R. J. & McAllan, A. B. (1983). A comparison of the chemical composition of mixed bacteria harvested from liquid and solid fractions of rumen digesta. British Journal of Nutrition 50, 701–709.CrossRefGoogle ScholarPubMed
Millar, E. L. (1982). Protein Contribution of Feedstuffs for Ruminants (ed. Millar, E. L., Pike, J. M. and Van Ed, A. J. H.), pp. 18–35. London: Butterworths.CrossRefGoogle Scholar
Negi, S. S., Singh, B. & Makkar, H. P. S. (1988). Rumen degradability of nitrogen in typical cultivated grasses and leguminous fodders. Animal Feed Science and Technology (In the Press).CrossRefGoogle Scholar
Nocek, J. E., Cummins, K. A. & Polan, C. E. (1979). Ruminal disappearance of crude protein and dry matter in feeds and combined effects of formulated rations. Journal of Dairy Science 62, 1587–1598.CrossRefGoogle Scholar
Ørskov, E. R. & McDonald, I. (1979). The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. Journal of Agricultural Science 92, 499–503.CrossRefGoogle Scholar
Ørskov, E. R., Hovell, F. D. Deb. & Mould, F. (1980). The use of the nylon bag technique in the evaluation of feedstuffs. Tropical Animal Production 5, 195–213.Google Scholar
Pal, R. N. & Negi, S. S. (1977 a). Utilization of ureasupplemented alkali-sprayed low grade grass hay by cattle. Indian Journal of Animal Science 47, 66–72.Google Scholar
Pal, R. N. & Negi, S. S. (1977 b). Utilization of alkalisprayed paddy straw supplemented with urea or sparingly soluble urea formaldehyde complex by cattle. Indian Journal of Animal Science 47, 782–789.Google Scholar
Pal, R. N. & Negi, S. S. (1978). Effect or supplementing urea/urea derivatives on the utilization of nutrients by bullocks fed untreated and alkali sprayed wheat straw. Indian Journal of Animal Science 48, 656–661.Google Scholar
Prichard, R. N. & Males, J. R. (1985). Effect of crude protein and ruminal ammonia·N on digestibility and ruminal outflow in beef cattle fed wheat straw. Journal of Animal Science 60, 822–831.CrossRefGoogle Scholar
Reddy, E. P. & Prasad, D. A. (1985). Estimation oreffective nitrogen degradability of protein supplements using nylon bag technique. Indian Journal of Animal Nutrition 2, 1–6.Google Scholar
Sampath, K. T. & Sivaraman, E. (1985). In situ dry matter disappearance and protein degradability of certain cakes in the rumen of cattle. Indian Journal of Animal Nutrition 2, 141–148.Google Scholar
Sen, K. C., Ray, S. N. & Ranjhan, S. K. (1978). Nutritive Values of Indian Cattle Feeds and the Feeding of Animals. New Delhi: Indian Council of Agricultural Research.Google Scholar
Snedecor, G. W. & Cochran, W. G. (1967). Statistical Methods, 6th edn.Ames, Iowa: Iowa State University Press.Google Scholar
Stern, M. D. & Satter, L. D. (1984). Evaluation of N solubility and dacron bag technique as methods for estimating protein degradation in the rumen. Journal of Animal Science 58, 714–724.CrossRefGoogle Scholar
Uden, P. & Van Soest, P. J. (1984). Investigations of the in situ bag technique and a comparison of the fermentation in heifers, sheep, ponies and rabbits. Journal of Animal Science 58, 213–224.CrossRefGoogle Scholar