Hostname: page-component-586b7cd67f-t7czq Total loading time: 0 Render date: 2024-11-29T18:32:46.575Z Has data issue: false hasContentIssue false
  • English
  • Français

Adenosine 5′-triphosphate and adenylate energy charge in sheep digesta

Published online by Cambridge University Press:  27 March 2009

R. J. Wallace  and
Aileen A. West
R. J. Wallace
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen, AB2 9SB
Aileen A. West
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen, AB2 9SB
Get access Rights & Permissions [Opens in a new window]

Summary

The concentrations of adenosine 5′-mono-, -di-, and -triphosphate (AMP, ADP and ATP) in sheep digesta were measured by a new bioluminescence method. ATP in rumen digesta varied with time after feeding and diet, and most markedly according to the size of the population of ciliate protozoa: the ATP content of rumen digesta from ciliate-free sheep was less than a quarter of that of faunated animals receiving the same diet. The adenylate energy charge (EC) (ATP + ADP/[ATP + ADP + AMP]), an indicator of metabolic activity, was high (0·77–0·94) in all rumen samples, but did not appear to be as useful a measurement of activity as ATP alone. As digesta passed along the alimentary tract, the ATP content decreased progressively, from 0·9–2·2 μmol/g dry matter in the rumen to 0·5–1·0μmol/g in the abomasum and duodenum, 07middot;03–0·08 μmol/g in ileal digesta and 0·01–0·02 μmol/g in faeces. EC also tended to decrease, further emphasizing the large decrease in microbial activity which occurs in the hindgut.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 98 , Issue 3 , June 1982 , pp. 523 - 528
Copyright
Copyright © Cambridge University Press 1982

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Eadie, J. M. (1967). Studies on the ecology of certain rumen ciliate protozoa. Journal of General Microbiology 49, 175–194.CrossRefGoogle ScholarPubMed
Erfle, J. D., Mahadevan, S. & Sauer, F. D. (1979). Effect of diet quality on adenosine 5′-triphosphate concentration and adenylate energy charge of rumen microbes from faunated cows. Journal of Dairy Science 62, 284–291.CrossRefGoogle Scholar
Erfle, J. D., Mahadevan, S. & Sauer, F. D. (1981). Relationship between adenylate energy charge, rumen volatile fatty acid concentrations, and rates of production and dry matter digestibility in the cow. Journal of Animal Science 64, 634–642.Google Scholar
Forsberg, C. W. & Lam, K. (1977). Use of adenosine 5′-triphosphate as an indicator of the microbiota biomass in rumen contents. Applied and Environmental Microbiology 33, 528–537.CrossRefGoogle ScholarPubMed
Jensen, K. & Wolstrup, J. (1977). Effect of feeding frequency on fermentation pattern and microbial activity in the bovine rumen. Acta Veterinaria Scandinavica 18, 108–121.CrossRefGoogle ScholarPubMed
Knowles, C. J. (1977). Microbial metabolic regulation by adenine nucleotide pools. In Microbial Energetics (ed. Haddock, B. A. and Hamilton, W. A.), pp. 241–283. Cambridge University Press.Google Scholar
Mason, V. C. & White, F. (1971). The digestion of bacterial mucopeptide constituents in the sheep. 1. The metabolism of 2,6-diaminopimelic acid. Journal of Agricultural Science, Cambridge 77, 91–98.CrossRefGoogle Scholar
Pounden, W. D., Ferguson, L. C. & Hibbs, J. W. (1950). The digestion of rumen microorganisms by the host animals. Journal of Dairy Science 33, 565–572.CrossRefGoogle Scholar
Stern, M. D. & Hoover, W. H. (1979). Methods for determining and factors affecting rumen microbial protein synthesis: a review. Journal of Animal Science 49, 1590–1603.CrossRefGoogle Scholar
Ulyatt, M. J., Dellow, D. W., Reid, C. S. W. & Bauchop, T. (1975). Structure and function of the large intestine of ruminants. In Digestion and Metabolism in the Ruminant (ed. McDonald, I. W. and Warner, A. C. I.), pp. 119–133. Armidale, N. S. W. 2351, Australia: University of New England Publishing Unit.Google Scholar
Wainman, F. W., Smith, J. S. & Dewey, P. J. S. (1975). The nutritive value for sheep of ruminant diet AA6, a complete cobbed diet containing 30% barley straw. Journal of Agricultural Science, Cambridge 84, 109–111.CrossRefGoogle Scholar
Wolstrup, J. & Jensen, K. (1976). Adenosine triphosphate in the bovine rumen during maximum nutrient supply and starvation. Journal of Applied Bacteriology 41, 243–250.CrossRefGoogle Scholar
Wolstrup, J. & Jensen, K. (1978). Adenosine triphosphate and deoxyribonucleio acid in the alimentary tract of cattle fed different nitrogen sources. Journal of Applied Bacteriology 45, 49–56.CrossRefGoogle Scholar
Wolstrup, J., Jensen, K. & Just, A. (1979). ATP and DNA as microbial parameters in the alimentary tract. Annales de Recherches Vitérinaires 10, 283–285.Google ScholarPubMed