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Wool follicles initiate, develop and produce wool fibres in ovine fetal skin grafts

Published online by Cambridge University Press:  27 March 2009

C. E. McCloghry ,
D. E. Hollis ,
K. A. Raphael ,
R. C. Marshall ,
A. Foldes ,
J. P. Kennedy  and
P. C. Wynn
C. E. McCloghry
Affiliation:
Division of Animal Production, CSIRO, PO Box 239, Blacktown, NSW 2148, Australia
D. E. Hollis
Affiliation:
Division of Animal Production, CSIRO, PO Box 239, Blacktown, NSW 2148, Australia
K. A. Raphael
Affiliation:
Division of Animal Production, CSIRO, PO Box 239, Blacktown, NSW 2148, Australia
R. C. Marshall
Affiliation:
Division of Wool Technology, CSIRO, 343 Royal Parade, Parkville, VIC 3052, Australia
A. Foldes
Affiliation:
Division of Animal Production, CSIRO, PO Box 239, Blacktown, NSW 2148, Australia
J. P. Kennedy
Affiliation:
Department of Wool and Animal Science, University of New South Wales, PO Box I, Kensington, NSW 2033, Australia
P. C. Wynn
Affiliation:
Department of Animal Science, University of Sydney, Camden, NSW 2570, Australia
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Summary

Research into the mechanisms that control the initiation and development of wool follicles has been severely hampered by the inaccessibility of fetal skin in utero. The aims of this study were to determine whether ovine fetal skin, when grafted onto the athymic nude mouse (nu/nu), could be maintained outside the uterine environment and, if so, whether it would retain its ability to initiate and develop wool follicles capable of producing wool fibres.

Skin was removed from the mid-side region of ovine fetuses on days 45, 55, 65, 75, 85 and 95 of gestation, and transferred to graft beds prepared on anaesthetized nude mice. After developing for 20 days on the recipients, the grafts were excised for histological examination. Control ovine fetal skin was also obtained at the above times and from fetuses on days 105 and 115, and similarly processed for histological examination.

Fetal skin at all ages was successfully grafted onto nude mice; 90% of all grafts were accepted and maintained by the recipients. Follicle initiation and/or development occurred in all grafts, including those formed from day 45 fetal skin, collected and grafted prior to follicle initiation in vivo. The number of follicles in grafted skin was reduced compared to that in control fetal skin of equivalent age; however, follicle development was generally accelerated. Follicle initiation and development occurred predominantly in the peripheral zone of the grafts. Some of the follicles present in the skin at grafting were lost due to the grafting procedure, while others continued to develop and produce wool fibres. Follicle development varied considerably between grafts. All grafted skin exhibited premature loss of the periderm layer and cornification of the epidermis, probably in response to the exposure of the skin surface to the atmosphere. There was a notable absence of both arrector pili muscles and sweat glands associated with graft follicles, and a retardation of sebaceous gland development.

The grafting technique developed in this study has enabled ovine fetal skin to be maintained outside the uterine environment for extended periods of time and may provide an improved means for future investigation of wool follicle initiation and development.

Type
Animals
Information
The Journal of Agricultural Science , Volume 121 , Issue 2 , October 1993 , pp. 247 - 253
Copyright
Copyright © Cambridge University Press 1993

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References

REFERENCES

Corbett, J. L. (1979). Variation in wool growth with physiological state. In Physiological and Environmental Limitations to Wool Growth (Eds Black, J. L. & Reis, P. J.), pp. 79–98. Armidale, Australia: University of New England Publishing Unit.Google Scholar
Hardy, M. H. & Lyne, A. G. (1956 a). The pre-natal development of wool follicles in Merino sheep. Australian Journal of Biological Sciences 9, 423–441.CrossRefGoogle Scholar
Hardy, M. H. & Lyne, A. G. (1956 b). Studies on the development of wool follicles in tissue culture. Australian Journal of Biological Sciences 9, 559–574.CrossRefGoogle Scholar
Hickman, J. L., Rudall, K. M. & Wickham, G. A. (1962). Storage of foetal sheep skin and its growth as an autograft on the lamb. Nature 194, 394–395.CrossRefGoogle ScholarPubMed
Lyne, A. G. & Hollis, D. E. (1972). The structure and development of the epidermis in sheep fetuses. Journal of Ultrastruclure Research 38, 444–458.CrossRefGoogle ScholarPubMed
Lyne, A. G. & Hollis, D. E. (1980). Effects of grafting the skin in Merino sheep, with special reference to the growth of wool and hair. Australian Journal of Biological Sciences 33, 309–328.CrossRefGoogle Scholar
Manning, D. D., Reed, N. D. & Shifler, C. F. (1973). Maintenance of skin xenographs of widely divergent phylogenic origin on congenital athymic (nude) mice. Journal of Experimental Medicine 138, 488–494.CrossRefGoogle Scholar
Marshall, R. C. & Gillespie, J. M. (1982). Comparison of samples of human hair by two-dimensional electrophoresis. Journal of the Forensic Science Society 22, 377–385.CrossRefGoogle Scholar
Raphael, K. A. & Pennycuik, P. R. (1980). The site of action of the naked locus (N) in the mouse as determined by dermal-epidermal recombinations. Journal of Embryology and Experimental Morphology 57, 143–153.Google ScholarPubMed
Reed, N. D. & Manning, D.D. (1978). Present studies of xenotransplantation of nonmalignant tissue to the nude mouse. In The Nude Mouse in Experimental and Clinical Research (Eds Fogh, J. & Giovanella, B. C.), pp. 167–187. New York: Academic Press.Google Scholar
Rudall, K. M. & Wickham, G. A. (1965). Development of wool follicles and fibres on autoplastic grafts of stored foetal lamb skin. In Biology of the Skin and Hair Growth (Eds Lyne, A. G. & Short, B. F.), pp. 75–88. Sydney, Australia: Angus and Robertson.Google Scholar
Rugh, R. (1968). In The Mouse – its Reproduction and Development, pp. 297. Minneapolis, USA: Burgess Publishing Company.Google Scholar