Published online by Cambridge University Press: 27 March 2009
Initial pH of buffalo semen as measured electrometrically is 7.2 ± 0.025 and the buffering capacity 1.40 ± 0.026. Sulphamezathine in glucose–egg yolk–bicarbonate diluent depressed fructolysis in buffalo semen. This depression is considered to be a factor in the better maintenance of motility in this than in other media investigated. The more alkaline pH of this medium also appears to be beneficial in preserving motility on storage.