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Root formation in cuttings of Dioscorea composita

Published online by Cambridge University Press:  27 March 2009

Ana Maria Viana
Affiliation:
Departamento de Fisiologia Vegetal, Caixa Postal 6109, Universidade de Campinas, 13081, Campinas, SP, Brazil
G. M. Felippe
Affiliation:
Departamento de Fisiologia Vegetal, Caixa Postal 6109, Universidade de Campinas, 13081, Campinas, SP, Brazil

Summary

The tubers of Dioscorea composita Hemsl. contain a high level of diosgenin, and hence there is economic interest in this plant. Levels of diosgenin vary greatly in different clones of the species. Vegetative propagation was studied with a view to obtaining plants with high levels of diosgenin. In this study cuttings from 1-year-old vegetative plants were used for propagation. The cuttings were treated with a single application of indole-3-butyric acid (IBA) and several applications of 6 benzyladenine (6-BA). Rooting was never observed in untreated cuttings. Cuttings treated with IBA produced callus and roots. Early application of 6-BA delayed senescence but inhibited callus and root formation. New plants were obtained with one application of IBA followed by applications of 6-BA at 48 h intervals initiated 2 weeks after IBA application. In some experiments, all cuttings treated this way produced new plants.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1988

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