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Rate of production of protozoa in the rumen and the flow of protozoal nitrogen to the duodenum in sheep and cattle given a pelleted diet of lucerne hay and barley

Published online by Cambridge University Press:  27 March 2009

B. S. Punia ,
J. Leibholz  and
G. J. Faichney
B. S. Punia
Affiliation:
Department of Animal Science, University of Sydney, Camden, NSW 2570, Australia
J. Leibholz
Affiliation:
Department of Animal Science, University of Sydney, Camden, NSW 2570, Australia
G. J. Faichney
Affiliation:
CSIRO, Division of Animal Production, PO Box 239, Blacktown, NSW 2148, Australia
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Summary

Two crossbred wethers (c. 45 kg) and two Hereford heifers (c. 215 kg) were fitted with permanent cannulas in the rumen and abomasum and given a diet of lucerne hay and barley (60:40). Organic matter (OM) intakes were 0·67 kg/day for sheep and 3·14 kg/day for cattle.

The mean numbers of protozoa in the rumen fluid were 9·3 × 105/ml for sheep and 7·4 × 105/ml for cattle. 14C-labelled protozoa were prepared by incubating rumen fluid in vitro with [14C]methyl choline and then isolating them by sedimentation and differential centrifugation. The labelled protozoa were returned to the rumen of each of the donor animals in a single injection. The specific radioactivity in mixed protozoa isolated from the rumen was measured for 3 days. The protozoal pool size was 10 and 12 g N in the sheep and 64 and 44 g N in the cattle. Protozoal N contributed, on average, 48% of the total N in the rumen.

Production of protozoal N in the rumen was 9·5 and 11·2 g/day for the sheep and 49·5 and 38·6 g/day for the cattle. The flow of protozoal N from the abomasum was calculated to be 1·6 and 2·9 g/day for sheep and 17·3 and 13·3 g/day for cattle. Thus, on average, 79% of the protozoal N was recycled within the rumen of the sheep and 65% within the rumen of the cattle. Protozoal N made up 11–20% of total N flow from the abomasum. The turnover times of 14C-labelled protozoa injected into the rumen of the sheep and the cattle were similar (26·2 and 26·8 h for sheep; 30·8 and 27·5 h for cattle) and were similar to those for protozoa isolated from the rumen of sheep after direct intraruminal injection of [14C]methyl choline (28 and 36 h).

Type
Animals
Information
The Journal of Agricultural Science , Volume 118 , Issue 2 , April 1992 , pp. 229 - 236
Copyright
Copyright © Cambridge University Press 1992

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References

REFERENCES

Broad, T. E. & Dawson, R. M. C. (1976). Role of choline in the nutrition of the rumen protozoon Entodinium caudatum. Journal of General Microbiology 92, 391–397.CrossRefGoogle ScholarPubMed
Coleman, G. S. (1979). The role of rumen protozoa in the metabolism of ruminants given tropical feeds. Tropical Animal Production 4, 199–213.Google Scholar
Faichney, G. J. (1975). The use of markers to partition digestion within the gastrointestinal tract of ruminants. In Digestion and Metabolism in the Ruminant (Eds McDonald, I. W. & Warner, A. C. I.), pp. 277–291. Armidale, NSW, Australia: The University of New England Publishing Unit.Google Scholar
Faichney, G. J. (1980 a). The use of markers to measure digesta flow from the stomach of sheep fed once daily. Journal of Agricultural Science, Cambridge 94, 313–318.CrossRefGoogle Scholar
Faichney, G. J. (1980 b). Measurement in sheep of the quantity and composition of rumen digesta and of the fractional outflow rates of digesta constituents. Australian Journal of Agricultural Research 31, 1129–1137.CrossRefGoogle Scholar
Harrison, D. G. & McAllan, A. B. (1980). Factors affecting microbial growth yields in the reticulo-rumen. In Digestive Physiology and Metabolism in Ruminants (Eds Ruckebusch, Y. & Thivend, P.), pp. 205–226. Lancaster: MTP Press.CrossRefGoogle Scholar
Harrison, D. G., Beever, D. E. & Osbourn, D. F. (1979). The contribution of protozoa to the protein entering the duodenum of sheep. British Journal of Nutrition 41, 521–527.CrossRefGoogle Scholar
Hungate, R. E. (1966). The Rumen and its Microbes. New York and London: Academic Press.Google Scholar
Leng, R. A. (1982). Dynamics of protozoa in the rumen of sheep. British Journal of Nutrition 48, 399–415.CrossRefGoogle ScholarPubMed
Leng, R. A. (1989). Dynamics of protozoa in the rumen. In The Role of Protozoa and Fungi in Ruminant Digestion (Eds Nolan, J. V., Leng, R. A. & Demeyer, D. I.), pp. 51–58. Armidale, NSW Australia: Penambul Books.Google Scholar
Leng, R. A., Gill, M., Kempton, T. J., Rowe, J. B., Nolan, J. V., Stachiw, S. J. & Preston, T. R. (1981). Kinetics of large ciliate protozoa in the rumen of cattle given sugarcane diets. British Journal of Nutrition 46, 371–384.CrossRefGoogle Scholar
Leng, R. A., Nolan, J. V., Cumming, G., Edwards, S. R. & Graham, C. A. (1984). The effects of monensin on the pool size and turnover rate of protozoa in the rumen of sheep. Journal of Agricultural Science, Cambridge 102, 609–613.CrossRefGoogle Scholar
Leng, R. A., Dellow, D. W. & Waghorn, G. (1986). Dynamics of large ciliate protozoa in the rumen of cattle fed on diets of freshly cut grass. British Journal of Nutrition 56, 455–462.CrossRefGoogle ScholarPubMed
Moir, R. J. (1984). Why an omasum? In Ruminant Physiology: Concepts and Consequences (tribute to Moir, R. J.), pp. 85–92. Proceedings of a Symposium, University of Western Australia.Google Scholar
Punia, B. S. & Leibholz, J. (1984). Protozoal nitrogen in the stomach of cattle. Canadian Journal of Animal Science 64 (Supplement), 24–25.CrossRefGoogle Scholar
Punia, B. S., Leibholz, J. & Faichney, G. J. (1984 a). The flow of protozoal nitrogen to the omasum of cattle. Proceedings of the Australian Society of Animal Production 15, 732.Google Scholar
Punia, B. S., Leibholz, J. & Faichney, G. J. (1984 b). Protozoal numbers in the rumen and omasum of cattle fed kikuyu grass. Proceedings of the Australian Society of Animal Production 15, 733.Google Scholar
Punia, B. S., Leibholz, J. & Faichney, G. J. (1987). The role of rumen protozoa in the utilization of paspalum (Paspalum dilatatum) hay by cattle. British Journal of Nutrition 57, 395–406.CrossRefGoogle ScholarPubMed
Rahnema, S. H. & Theurer, B. (1986). Comparison of various amino acids for estimation of microbial nitrogen in digesta. Journal of Animal Science 63, 603–612.CrossRefGoogle ScholarPubMed
Rows, J. B., Davies, A. & Broom, A. W. J. (1985). Quantitative effects of defaunation on rumen fermentation and digestion in sheep. British Journal of Nutrition 54, 105–119.Google Scholar
Shipley, R. A. & Clarke, R. E. (1972). Tracer Methods in in vivo kinetics. New York: Academic Press.Google Scholar
Steinhour, W. D., Stokes, M. R., Clark, J. H., Rogers, J. A., Davis, C. L. & Nelson, D. R. (1982). Estimation of the proportion of non-ammonia-nitrogen reaching the lower gut of the ruminant derived from bacterial and protozoal nitrogen. British Journal of Nutrition 48, 417–431.CrossRefGoogle ScholarPubMed
Van Soest, P. J. (1982). Nutritional Ecology of the Ruminant. Corvallis, USA: Cornell University, O & B Books.Google Scholar
Weller, R. A. & Pilgrim, A. F. (1974). Passage of protozoa and volatile fatty acids from the rumen of the sheep and from a continuous in vitro fermentation system. British Journal of Nutrition 32, 341–351.CrossRefGoogle ScholarPubMed