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Phosphoglucoisomerase-2 allozymes for distinguishing perennial ryegrass cultivars in binary mixtures

Published online by Cambridge University Press:  27 March 2009

P. M. Sanders ,
D. J. Barker  and
G. S. Wewala
P. M. Sanders
Affiliation:
Grasslands Division, DSIR, Palmerston North, New Zealand
D. J. Barker
Affiliation:
Grasslands Division, DSIR, Palmerston North, New Zealand
G. S. Wewala
Affiliation:
Applied Mathematics Division, DSIR, Palmerston North, New Zealand
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Summary

The analysis of Kennedy et al. (1985) was applied to 150 individuals from each of six plots containing mixtures of the perennial ryegrass (Lolium perenne L.) cultivar Grasslands Nui and the resident ryegrass population at Rawhiti, Central Wairarapa, New Zealand. Four alleles (A, B, C and D) were identified at the phosphoglucoisomerase-2 allozyme locus in the two ryegrass populations. Using the maximum likelihood method, non-linear models were fitted to calculate the percentage contribution of Grasslands Nui to the mixtures. The contribution of Grasslands Nui to the six evaluation plots ranged from 5 to 44%, with S.E.S of 11–17%. The lowest contribution occurred under set stocking with sheep and a low fertilizer regime. The persistence of Grasslands Nui under contrasting grazing managements and fertilizer regimes in this dry environment, and aspects of enzyme electrophoresis application to field trials, are discussed.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 112 , Issue 2 , April 1989 , pp. 179 - 184
Copyright
Copyright © Cambridge University Press 1989

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