Hostname: page-component-cd9895bd7-jn8rn Total loading time: 0 Render date: 2024-12-23T15:30:40.673Z Has data issue: false hasContentIssue false
  • English
  • Français

Overexpression of the MhTGA2 gene from crab apple (Malus hupehensis) confers increased tolerance to salt stress in transgenic apple (Malus domestica)

Published online by Cambridge University Press:  18 March 2013

X. DU ,
B. DU ,
X. CHEN ,
S. ZHANG ,
Z. ZHANG  and
S. QU
X. DU
Affiliation:
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
B. DU
Affiliation:
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
X. CHEN
Affiliation:
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
S. ZHANG
Affiliation:
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
Z. ZHANG
Affiliation:
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
S. QU*
Affiliation:
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
*
*To whom all correspondence should be addressed. Email: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

Leaves of Nagafu No. 6 were transformed by the Agrobacterium tumefaciens-mediated method, and 158 hyg-resistant buds were obtained. Using fresh leaf tissue, seven positive lines were obtained by polymerase chain reaction (PCR) with total DNA, and four by reverse transcriptase–polymerase chain reaction (RT–PCR) with total RNA. The results showed that the MhTGA2 gene was successfully transformed into Nagafu No. 6 plants and expressed. At 4 months after transplanting, photosynthetic parameters of young leaves were determined using transgenic and control plants prior to the determination of leaf surface characteristics (epidermal structure and stomatal distribution) by scanning electronic microscopy. The results showed that the net photosynthesis rate, the instantaneous carboxylation rate and stomatal conductance of transgenic plants were higher than the control, whereas the internal carbon dioxide (CO2) concentrations of the transgenic plants were lower. Under non-saline conditions, the stomata of the transgenic plants were better distributed than in the control. Under saline conditions, the epidermal structure of the control leaves was severely hypohydrated and the quantity of stomata was significantly decreased. Conversely, the epidermal structure of transgenic leaves was not significantly altered, indicating salt-stress tolerance. The overall results suggested that MhTGA2 may play a crucial role in the response to salt stress in Nagafu No. 6.

Type
Crops and Soils Research Papers
Information
The Journal of Agricultural Science , Volume 152 , Issue 4 , August 2014 , pp. 634 - 641
Copyright
Copyright © Cambridge University Press 2013 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Belfanti, E., Silfverberg-Dilworth, E., Tartarini, S., Patocchi, A., Barbieri, M., Zhu, J., Vinatzer, B. A., Gianfranceschi, L., Gessler, C. & Sansavini, S. (2004). The HcrVf2 gene from a wild apple confers scab resistance to a transgenic cultivated variety. Proceedings of the National Academy of Sciences of the United States of America 101, 886–890.Google Scholar
Bolar, J. P., Norelli, J. L., Wong, K. W., Hayes, C. K., Harman, G. E. & Aldwinckle, H. S. (2000). Expression of endochitinase from trichoderma harzianum in transgenic apple increases resistance to apple scab and reduces vigor. Phytopathology 90, 72–77.Google Scholar
Faize, M., Malony, M., Dupuis, F., Chevalier, M., Parisi, L. & Chevreau, E. (2003). Chitinases of Trichoderma atroviride induce scab resistance and some metabolic changes in two cultivars of apple. Phytopathology 93, 1496–1504.CrossRefGoogle ScholarPubMed
Fujita, Y., Fujita, M., Satoh, R., Maruyama, K., Parvez, M. M., Seki, M., Hiratsu, K., Ohme-Takagi, M., Shinozaki, K. & Yamaguchi-Shinozaki, K. (2005). AREB1 is a transcription activator of novel ABRE dependent ABA signaling that enhances drought stress tolerance in Arabidopsis. Plant Cell 17, 3470–3488.Google Scholar
Jakoby, M., Weisshaar, B., Dröge-Laser, W., Vicente-Carbajosa, J., Tiedemann, J., Kroj, T. & Parcy, F. (2002). bZIP transcription factors in Arabidopsis. Trends in Plant Science 7, 106–111.Google Scholar
Kang, J. Y., Choi, H. I., Im, M. Y. & Kim, S. Y. (2002). Arabidopsis basic leucine zipper proteins that mediate stress-responsive abscisic acid signaling. Plant Cell 14, 343–357.Google Scholar
Karpinski, S., Wingsle, G., Karpinska, B. & Hällgren, J-E. (2004). Redox sensing of photooxidative stress and acclimatory mechanisms in plants. In Regulation of Photosynthesis (Eds Aro, E-M. & Andersson, B.), pp. 469–486. Dordrecht, The Netherlands: Kluwer Academic Publishers.Google Scholar
Lee, S. C., Choi, H. W., Hwang, I. S., du Choi, S. & Hwang, B. K. (2006). Functional roles of the pepper pathogen-induced bZIP transcription factor, CAbZIP1, in enhanced resistance to pathogen infection and environmental stresses. Planta 224, 1209–1225.CrossRefGoogle ScholarPubMed
Liao, Y., Zou, H. F., Wei, W., Hao, Y. J., Tian, A. G., Huang, J., Liu, Y. F., Zhang, J. S. & Chen, S. Y. (2008). Soybean GmbZIP44, GmbZIP62 and GmbZIP78 genes function as negative regulator of ABA signaling and confer salt and freezing tolerance in transgenic Arabidopsis. Planta 228, 225–240.CrossRefGoogle ScholarPubMed
Nijhawan, A., Jain, M., Tyagi, A. K. & Khurana, J. P. (2008). Genomic survey and gene expression analysis of the basic leucine zipper transcription factor family in rice. Plant Physiology 146, 333–350.Google Scholar
Parsons, E., Bole, B., Hall, D. J. & Thomas, W. D. E. (1974). A comparative survey of techniques for preparing plant surfaces for the scanning electron microscope. Journal of Microscopy 101, 59–75.Google Scholar
Rodriguez-Uribe, L. & O'Connell, M. A. (2006). A root-specific bZIP transcription factor is responsive to water deficit stress in tepary bean (Phaseolus acutifolius). Journal of Experimental Botany 57, 1391–1398.Google Scholar
Seong, E. S., Kwon, S. S., Ghimire, B. K., Yu, C. Y., Cho, D. H., Lim, J. D., Kim, K. S., Heo, K., Lim, E. S., Chung, I. M., Kim, M. J. & Lee, Y. S. (2008). LebZIP2 induced by salt and drought stress and transient overexpression by Agrobacterium. BMB Reports 41, 693–698.CrossRefGoogle ScholarPubMed
Shearer, H. L., Wang, L. P., DeLong, C., Despres, C. & Fobert, P. R. (2009). NPR1 enhances the DNA binding activity of the Arabidopsis bZIP transcription factor TGA7. Botany 87, 561–570.Google Scholar
Stankovic, B., Vian, A., Henry-Vian, C. & Davies, E. (2000). Molecular cloning and characterization of a tomato cDNA encoding a systemically wound-inducible bZIP DNA binding protein. Planta 212, 60–66.Google Scholar
Wang, J. Z., Xue, X. M. & Lu, C. (2010 a). China's apple production situation and development strategy. Shandong Agricultural Sciences 6, 117–119.Google Scholar
Wang, Y. C., Gao, C., Liang, Y., Wang, C., Yang, C. & Liu, G. (2010 b). A novel bZIP gene from Tamarixhispida mediates physiological responses to salt stress in tobacco plants. Journal of Plant Physiology 167, 222–230.Google Scholar
Chen, X. K., Zhang, J. Y., Zhang, Z., Du, X. L., Du, B. B. & Qu, S. C. (2012). Overexpressing MhNPR1 in transgenic Fuji apples enhances resistance to apple powdery mildew. Molecular Biology Reports 39, 8083–8089.Google Scholar
Zhang, J. Y., Qu, S. C., Du, X. L., Qiao, Y. S., Cai, B. H., Guo, Z. R. & Zhang, Z. (2012). Overexpression of the Malus hupehensis MhTGA2 gene, a novel bZIP transcription factor for increased tolerance to salt and osmotic stress in transgenic tobacco. International Journal of Plant Science 173, 441–453.Google Scholar
Zhang, X., Wollenweber, B., Jiang, D., Liu, F. & Zhao, J. (2008). Water deficit sand heat shock effects on photosynthesis of a transgenic Arabidopsis thaliana constitutively expressing ABP9, a bZIP transcription factor. Journal of Experimental Botany 59, 839–848.Google Scholar
Zhang, Z., Sun, A. J., Cong, Y., Sheng, B. C., Yao, Q. H. & Cheng, Z. M. (2006). Agrobacterium-mediated transformation of the apple rootstock Malus MicroMalus makino with the Rolc gene. In Vitro Cellular and Development Biology – Plant 42, 491–497.Google Scholar
Zou, M., Guan, Y., Ren, H., Zhang, F. & Chen, F. (2008). A bZIP transcription factor, OsABI5, is involved in rice fertility and stress tolerance. Plant Molecular Biology 66, 675–683.CrossRefGoogle ScholarPubMed