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Impact of dynamics of faecal concentrations of plant and synthetic n-alkanes on their suitability for the estimation of dry matter intake and apparent digestibility in horses

Published online by Cambridge University Press:  09 August 2016

M. BACHMANN ,
M. WENSCH-DORENDORF ,
M. BULANG  and
A. ZEYNER
M. BACHMANN*
Affiliation:
Institute of Agricultural and Nutritional Sciences, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany
M. WENSCH-DORENDORF
Affiliation:
Institute of Agricultural and Nutritional Sciences, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany
M. BULANG
Affiliation:
Institute of Agricultural and Nutritional Sciences, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany
A. ZEYNER
Affiliation:
Institute of Agricultural and Nutritional Sciences, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany
*
*To whom all correspondence should be addressed. Email: [email protected]
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Summary

In horses, the quantity of faeces and the faecal concentrations of plant and synthetic alkanes are inconsistent throughout the day. The estimation of feed intake and digestibility can additionally be limited by irregular and incomplete faecal recovery of alkanes that are used as dietary markers. The correction of alkane concentrations minimizes the bias of estimates, but requires the determination of faeces quantity by total collection. However, in consideration of the dynamics of alkane concentrations in faeces, sampling at selected timeframes throughout a day may be useful in avoiding such correction. Five adult horses were fed a hay-based diet offered three times a day in equal amounts. Horses received a bolus with similar quantities of n-octacosane (C28), n-dotriacontane (C32) and n-hexatriacontane (C36) synthetic alkanes twice a day. Total faeces were quantified over 3 consecutive days. Dry matter intake (DMI), output (DMO) and digestibility (DMD) were determined from the total collection trial and additionally estimated for each of 12 equal timeframes throughout the day. The diurnal patterns of the single faeces quantity (SFQ) and faecal alkane concentrations were similar between horses and were repeated from day to day. The intra-day dynamic of SFQ was pronounced. The dynamic of the faecal concentration was much more pronounced when the alkane was administered twice instead of three times a day. The faecal recovery of alkanes that has been calculated from the total collection trial ranged from 82 ± 4·1% for C36 to 108 ± 11·1% for C28. Measured DMI was 12·0 kg/day, measured DMO was 5·9 kg/day and measured DMD was 0·51. Reliable estimates were obtained for DMI with 12·3 ± 0·79 kg/day for the combination of n-nonacosane (C29) and C28 and 12·1 ± 1·01 kg/day for the combination of n-tritriacontane (C33) and C28 at 2 h after administration, and 12·1 ± 0·96 kg/day for the combination of n-hentriacontane (C31) and C32 at 2 h prior to the morning meal, which included the first bolus administration. When calculated from DMO and DMD, DMI was 12·2 ± 0·89 kg/day for C29 and 12 ± 1·0 kg/day for C33 between 5 and 6 h after the morning meal. Estimates of DMD were unbiased between the 3rd and 4th hour after the morning meal with 0·52 ± 0·014 for C29 and 0·51 ± 0·021 for C33, respectively. The DMO was 5·7 ± 0·34 kg/day and 6·1 ± 0·43 kg/day when estimated 3–4 h after the 2nd meal, or prior to the 2nd bolus administration, using the product of SFQ and the daily defecation frequency or the synthetic alkanes, respectively. Knowledge of defecation dynamics might be helpful for simplifying experimental trials. They specifically followed intake dynamics, which can prospectively be used to select sampling timeframes. Based upon current results, a selection of two to three spot samples of faeces that are evenly distributed between 2 h before and 6 h after the morning meal, which was the time of bolus administration, allows for the greatest reliability. Defecation dynamics are probably less influenced by ration/bolus type, rate of exercise, or gut peristalsis, which nevertheless can result in individual shifts of optimal timeframes.

Type
Animal Research Papers
Information
The Journal of Agricultural Science , Volume 154 , Issue 7 , September 2016 , pp. 1291 - 1305
Copyright
Copyright © Cambridge University Press 2016 

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References

Bachmann, M., Wensch-Dorendorf, M., Mäder, K., Bulang, M. & Zeyner, A. (2016 a). Preparation of synthetic alkane waxes and investigations on their suitability for application as dietary markers in farm animals. Livestock Science 185, 110–116.Google Scholar
Bachmann, M., Wensch-Dorendorf, M., Wulf, M., Glatter, M., Siebmann, M., Bierögel, C., Schumann, E., Bulang, M., Aurich, C. & Zeyner, A. (2016 b). Bolus matrix for administration of dietary markers in horses. Livestock Science 185, 43–49.Google Scholar
Brosh, A., Henkin, Z., Rothman, S. J., Aharoni, Y., Orlov, A. & Arieli, A. (2003). Effects of faecal n-alkane recovery in estimates of diet composition. Journal of Agricultural Science, Cambridge 140, 93–100.Google Scholar
Castelán-Ortega, O. A., Estrada-Flores, J. G., Smith, D. G., Colunga, B., Solís-Méndez, A., Avilés-Nova, F., Ramirez, A. & García, I. (2007). Use of n-alkanes for estimation of voluntary intake and digestibility in donkeys (Equus asinus). Journal of Animal and Feed Sciences 16(Suppl. 2), 307–312.Google Scholar
Cuddeford, D. & Hughes, D. (1990). A comparison between chromium-mordanted hay and acid-insoluble ash to determine apparent digestibility of a chaffed, molassed hay/straw mixture. Equine Veterinary Journal 22, 122–125.Google Scholar
Dove, H. & Mayes, R. W. (1991). The use of plant wax alkanes as marker substances in studies of the nutrition of herbivores: a review. Australian Journal of Agricultural Research 42, 913–952.Google Scholar
Dove, H. & Mayes, R. W. (2006). Protocol for the analysis of n-alkanes and other plant-wax compounds and for their use as markers for quantifying the nutrient supply of large mammalian herbivores. Nature Protocols 1, 1680–1697.Google Scholar
Dove, H., Mayes, R. W. & Freer, M. (1996). Effects of species, plant part, and plant age on the n-alkane concentrations in the cuticular wax of pasture plants. Australian Journal of Agricultural Research 47, 1333–1347.Google Scholar
Drogoul, C., de Fombelle, A. & Julliand, V. (2001). Feeding and microbial disorders in horses: 2: effect of three hay:grain ratios on digesta passage rate and digestibility in ponies. Journal of Equine Veterinary Science 21, 487–491.CrossRefGoogle Scholar
Elwert, C., Kluth, H. & Rodehutscord, M. (2004). Effect of variable intake of alfalfa and wheat on faecal alkane recoveries and estimates of roughage intake in sheep. Journal of Agricultural Science, Cambridge 142, 213–223.Google Scholar
FASS (2010). Guide for the Care and Use of Agricultural Animals in Research and Teaching, 3rd edn. Champaign, IL, USA: Federation of Animal Science Societies. Available from: http://www.fass.org/docs/agguide3rd/Ag_Guide_3rd_ed.pdf (verified 25 February 2016).Google Scholar
Ferreira, L. M. M., Garcia, U., Rodrigues, M. A. M., Celaya, R., Dias-da-Silva, A. & Osoro, K. (2007). Estimation of feed intake and apparent digestibility of equines and cattle grazing on heathland vegetation communities using the n-alkane markers. Livestock Science 110, 46–56.Google Scholar
Ferreira, L. M. M., Celaya, R., García, U., Rodrigues, M. A. M. & Osoro, K. (2009). Differences between domestic herbivores species in alkane faecal recoveries and the accuracy of subsequent estimates of diet composition. Animal Feed Science and Technology 151, 128–142.Google Scholar
Fuchs, R., Militz, H. & Hoffmann, M. (1987). Untersuchungen zur Verdaulichkeit der Rohnährstoffe bei Pferden. 1. Mitteilung. Methoden zur Bestimmung der Verdaulichkeit. Archives of Animal Nutrition 37, 235–246.Google Scholar
GfE (2014). Empfehlungen zur Energie- und Nährstoffver-sorgung von Pferden. Frankfurt, Germany: DLG-Verlag.Google Scholar
Giráldez, F. J., Lamb, C. S., López, S. & Mayes, R. W. (2004). Effects of carrier matrix and dosing frequency on digestive kinetics of even-chain alkanes and implications on herbage intake and rate of passage studies. Journal of the Science of Food and Agriculture 84, 1562–1570.Google Scholar
Goachet, A. G., Philippeau, C., Varloud, M. & Julliand, V. (2009). Adaptations to standard approaches for measuring total tract apparent digestibility and gastro-intestinal retention time in horses in training. Animal Feed Science and Technology 152, 141–151.Google Scholar
Golonka, M. (2009). Analysis of chosen behavioral forms of Konik Polski horses from the Popielno Reserve. Annals of Warsaw University of Life Science – SGGW, Animal Science 46, 255–259.Google Scholar
Gudmundsson, O. & Thorhallsdottir, A. G. (1998). Evaluation of n-alkanes for intake and digestibility determination in horses. In Proceedings of the 9th European Intake Workshop (Eds Gibb, M. J. & Rutter, S. M.), pp. 49–52. Aberystwyth, UK: IGER.Google Scholar
Haenlein, G. F. W., Smith, R. C. & Yoon, Y. M. (1966). Determination of the fecal excretion rate of horses with chromic oxide. Journal of Animal Science 25, 1091–1095.CrossRefGoogle Scholar
Hyslop, J. J. (2003). Partitioning degradation of feeds between different segments of the equine digestive tract. In Emerging Equine Science (Ed. Alliston, J.), pp. 97–111. Occasional Publication of the British Society of Animal Science No. 32. Nottingham: Nottingham University Press.Google Scholar
Jensen, R. B., Brøkner, C., Bach Knudsen, K. E. & Tauson, A. H. (2010). Comparative study of the apparent total tract digestibility of fibre in Icelandic and Danish Warmblood horses. In The Impact of Nutrition on the Health and Welfare of Horses (Eds Ellis, A. D., Longland, A. C., Coenen, M. & Miraglia, N.), pp. 207–209. EAAP publication No. 128. Wageningen: Wageningen Academic Publishers.Google Scholar
Keli, A., Mayes, R. W. & de Vega, A. (2008). In vitro studies of the metabolism of [14C]-n-alkanes using ruminal fluid of sheep as substrate. Animal 2, 1748–1752.Google Scholar
Kienzle, E. & Zeyner, A. (2010). The development of a metabolizable energy system for horses. Journal of Animal Physiology and Animal Nutrition 94, 231–240.Google Scholar
Koch, K. & Ensikat, H.-J. (2008). The hydrophobic coatings of plant surfaces: epicuticular wax crystals and their morphologies, crystallinity and molecular self-assembly. Micron 39, 759–772.Google Scholar
Kolattukudy, P. E. & Hankin, L. (1966). Metabolism of a plant wax paraffin (n-nonacosane) in the rat. Journal of Nutrition 90, 167–174.CrossRefGoogle ScholarPubMed
Licitra, G., Hernandez, T. M. & Van Soest, P. J. (1996). Standardization of procedures for nitrogen fractionation of ruminant feeds. Animal Feed Science and Technology 57, 347–358.Google Scholar
Lin, L.-J., Luo, H.-L., Zhang, Y.-J. & Shu, B. (2007). The effects, in sheep, of dietary plant species and animal live weight on the faecal recovery rates of alkanes and the accuracy of intake and diet composition estimates obtained using alkanes as faecal markers. Journal of Agricultural Science, Cambridge 145, 87–94.Google Scholar
Mayes, R. W., Lamb, C. S. & Colgrove, P. M. (1986). The use of dosed and herbage n-alkanes as markers for the determination of herbage intake. Journal of Agricultural Science, Cambridge 107, 161–170.CrossRefGoogle Scholar
Mayes, R. W., Lamb, C. S. & Colgrove, P. M. (1988). Digestion and metabolism of dosed even-chain and herbage odd-chain n-alkanes in sheep. In Proceedings of the 12th General Meeting of the European Grassland Federation (Ed. European Grassland Federation), pp. 159–163. Wicklow, Ireland: Wicklow Press.Google Scholar
Molina, D. O., Matamoros, I. & Pell, A. N. (2004). Accuracy of estimates of herbage intake of lactating cows using alkanes: comparison of two types of capsules. Animal Feed Science and Technology 114, 241–260.Google Scholar
Oliván, M. & Osoro, K. (1999). Effect of temperature on alkane extraction from faeces and herbage. Journal of Agricultural Science, Cambridge 132, 305–312.Google Scholar
Oliván, M., Ferreira, L. M. M., Celaya, R. & Osoro, K. (2007). Accuracy of the n-alkane technique for intake estimates in beef cattle using different sampling procedures and feeding levels. Livestock Science 106, 28–40.Google Scholar
Ordakowski, A. L., Kronfeld, D. S., Holland, J. L., Hargreaves, B. J., Gay, L. S., Harris, P. A., Dove, H. & Sklan, D. (2001). Alkanes as internal markers to estimate digestibility of hay or hay plus concentrate diets in horses. Journal of Animal Science 79, 1516–1522.Google Scholar
Pagan, J. D., Harris, P. A., Brewster-Barnes, T., Duren, S. E. & Jackson, S. G. (1998). Exercise affects digestibility and rate of passage of all-forage and mixed diets in thoroughbred horses. Journal of Nutrition 128, 2704S–2707S.CrossRefGoogle ScholarPubMed
Patterson, D. P., Cooper, S. R., Freeman, D. W. & Teeter, R. G. (2002). Technical note: estimation of fecal output and dry matter digestibility using various chromic oxide marker methods in the horse. Professional Animal Scientist 18, 176–179.Google Scholar
Peiretti, P. G., Meineri, G., Miraglia, N., Mucciarelli, M. & Bergero, D. (2006). Intake and apparent digestibility of hay or hay plus concentrate diets determined in horses by the total collection of feces and n-alkanes as internal markers. Livestock Science 100, 189–194.Google Scholar
Rosenfeld, I., Austbø, D. & Volden, H. (2006). Models for estimating digesta passage kinetics in the gastrointestinal tract of the horse. Journal of Animal Science 84, 3321–3328.Google Scholar
Sales, J. (2012). A review on the use of indigestible dietary markers to determine total tract apparent digestibility of nutrients in horses. Animal Feed Science and Technology 174, 119–130.Google Scholar
Smith, D. G., Mayes, R. W., Hollands, T., Cuddeford, D., Yule, H. H., Malo Ladrero, C. M. & Gillen, E. (2007). Validating the alkane pair technique to estimate dry matter intake in equids. Journal of Agricultural Science, Cambridge 145, 273–281.CrossRefGoogle Scholar
Stevens, D. M., van Ryssen, J. B. J. & Marais, J. P. (2002). The use of n-alkane markers to estimate the intake and apparent digestibility of ryegrass and Kikuyu by horses. South African Journal of Animal Science 32, 50–56.Google Scholar
Takagi, H., Hashimoto, Y., Yonemochi, C., Asai, Y., Yoshida, T., Ohta, Y., Ishibashi, T. & Watanabe, R. (2002). Usefulness of chromic oxide index method for determination of digestibility of nutrients in feeds for thoroughbreds. Journal of Equine Science 13, 19–22.Google Scholar
Udén, P., Rounsaville, T. R., Wiggans, G. R. & Van Soest, P. J. (1982). The measurement of liquid and solid digesta retention in ruminants, equines and rabbits given timothy (Phleum pratense) hay. British Journal of Nutrition 48, 329–339.Google Scholar
Van Weyenberg, S., Sales, J. & Janssens, G. P. J. (2006). Passage rate of digesta through the equine gastrointestinal tract: a review. Livestock Science 99, 3–12.Google Scholar
VDLUFA (2012). Methodenbuch, Vol. 3, Die Chemische Untersuchung von Futtermitteln. Verband Deutscher Landwirtschaftlicher Untersuchungs- und Forschungsanstalten. Darmstadt: VDLUFA-Verlag.Google Scholar
Zeyner, A. & Kienzle, E. (2002). A method to estimate digestible energy in horse feed. Journal of Nutrition 132, 1771S–1773S.Google Scholar
Zeyner, A., Kirchhof, S., Susenbeth, A., Südekum, K.-H. & Kienzle, E. (2015). A new protein evaluation system for horse feed from literature data. Journal of Nutritional Science 4, e4. DOI: http://dx.doi.org/10.1017/jns.2014.66.Google Scholar
Zhang, Z., Li, G. & Shi, B. (2006). Physicochemical properties of collagen, gelatin and collagen hydrolysate derived from bovine limed split wastes. Journal of the Society of Leather Technologists and Chemists 90, 23–28.Google Scholar
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