Hostname: page-component-586b7cd67f-gb8f7 Total loading time: 0 Render date: 2024-11-25T04:22:29.131Z Has data issue: false hasContentIssue false
  • English
  • Français

Identification and characterization of two faba bean (Vicia faba L.) WRKY transcription factors and their expression analysis during salt and drought stress

Published online by Cambridge University Press:  03 November 2016

G. ABID ,
Y. MUHOVSKI ,
D. MINGEOT ,
M. N. SAIDI ,
M. AOUIDA ,
I. AROUA ,
M. M'HAMDI ,
F. BARHOUMI ,
S. REZGUI  and
M. JEBARA
G. ABID*
Affiliation:
Laboratory of Legumes, University of Tunis El Manar, Center of Biotechnology of Borj Cedria, 901, B-2050 Hammam-Lif, Tunisia
Y. MUHOVSKI
Affiliation:
Department of Life Sciences, Unit of Biological Engineering, Walloon Agricultural Research Centre, Chaussée de Charleroi, 234, B-5030 Gembloux, Belgium
D. MINGEOT
Affiliation:
Department of Life Sciences, Unit of Biological Engineering, Walloon Agricultural Research Centre, Chaussée de Charleroi, 234, B-5030 Gembloux, Belgium
M. N. SAIDI
Affiliation:
Center of Biotechnology of Sfax, Route Sidi Mansour Km 4, B.P 1177, 3018 Sfax, Tunisia
M. AOUIDA
Affiliation:
Laboratory of Legumes, University of Tunis El Manar, Center of Biotechnology of Borj Cedria, 901, B-2050 Hammam-Lif, Tunisia
I. AROUA
Affiliation:
Laboratory of Legumes, University of Tunis El Manar, Center of Biotechnology of Borj Cedria, 901, B-2050 Hammam-Lif, Tunisia
M. M'HAMDI
Affiliation:
Higher Agronomic Institute of Chott Mariem, BP 47, 4042 Chott Mariem, Sousse, Tunisia
F. BARHOUMI
Affiliation:
Laboratory of Legumes, University of Tunis El Manar, Center of Biotechnology of Borj Cedria, 901, B-2050 Hammam-Lif, Tunisia
S. REZGUI
Affiliation:
Laboratory of Legumes, University of Tunis El Manar, Center of Biotechnology of Borj Cedria, 901, B-2050 Hammam-Lif, Tunisia
M. JEBARA
Affiliation:
Laboratory of Legumes, University of Tunis El Manar, Center of Biotechnology of Borj Cedria, 901, B-2050 Hammam-Lif, Tunisia
*
*To whom all correspondence should be addressed. Email: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

Drought and salinity are two major environmental factors limiting faba bean growth, leading to considerable reduction in their productivity. The WRKY gene family act as major transcription factors that might play an important role in abiotic stress tolerance. In the present study, two partial sequences sharing significant homology with known WRKY genes were isolated from faba bean by polymerase chain reaction (PCR) amplification using degenerate primers targeting the well-conserved WRKY domain. The isolated WRKY gene fragments were designated as VfWRKY1 and VfWRKY2 showing 62% similarity between them. Sequence and phylogenetic analyses revealed that VfWRKY1 and VfWRKY2 belong to WRKY group I and could be grouped with their orthologues from other plant species. The gene expression profile of VfWRKY1 and VfWRKY2 in faba bean showed that they are significantly accumulated in various plant organs. Further, quantitative real-time PCR analysis showed that both transcripts were responsive to drought and salt stress, and also they are genotype dependent, meaning that different faba bean cultivars respond in a different way to drought and salt challenge. The expression patterns obtained suggest the important roles of VfWRKY1 and VfWRKY2 in drought and salt stress response and tolerance. This knowledge might be helpful in the identification of drought-tolerant cultivars and provide potential candidate markers for faba bean breeding in order to develop osmotic-stress-tolerant cultivars.

Type
Crops and Soils Research Papers
Information
The Journal of Agricultural Science , Volume 155 , Issue 5 , July 2017 , pp. 791 - 803
Check for updates
Copyright
Copyright © Cambridge University Press 2016 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Abdellatif, K. F., El Absawy, S. A. & Zakaria, A. M. (2012). Drought stress tolerance of faba bean as studied by morphological traits and seed storage protein pattern. Journal of Plant Studies 2, 47–54.Google Scholar
Amede, T., Schubert, S. & Stahr, K. (2003). Mechanisms of drought resistance in grain legumes. I. Osmotic adjustment. SINET: Ethiopian Journal of Science 26, 37–46.Google Scholar
Bakshi, M. & Oelmüller, R. (2014). WRKY transcription factors: jack of many trades in plants. Plant Signaling & Behavior 9, e27700–1. DOI: 10.4161/psb.27700 CrossRefGoogle ScholarPubMed
Baloglu, M. C., Inal, B., Kavas, M. & Unver, T. (2014). Diverse expression pattern of wheat transcription factors against abiotic stresses in wheat species. Gene 550, 117–122.CrossRefGoogle ScholarPubMed
Batool, N., Noor, T., Ilyas, N. & Shahzad, A. (2015). Molecular basis of salt stress tolerance in crop plants. Pure and Applied Biology 4, 80–88.CrossRefGoogle Scholar
Borrone, J. W., Kuhn, D. N. & Schnell, R. J. (2004). Isolation, characterization, and development of WRKY genes as useful genetic markers in Theobroma cacao . Theoretical and Applied Genetics 109, 495–507.CrossRefGoogle ScholarPubMed
Cabrall, P. D. S., dos Santos, L. N. S., Vieira, H. D., Soares, T. C. B., Bremenkamp, C. A. & Rodrigues, W. P. (2014). Effect of osmotic stress on the initial development of bean seedlings. American Journal of Plant Sciences 5, 1973–1982.CrossRefGoogle Scholar
Čereković, N., Jarret, D., Pagter, M., Cullen, D. W., Morris, J. M., Hedley, P. E., Brennan, R. & Petersen, K. K. (2015). The effects of drought stress on leaf gene expression during flowering in blackcurrant (Ribes nigrum L.). European Journal of Horticultural Science 80, 39–46.CrossRefGoogle Scholar
Chang, S., Puryear, J. & Cairney, J. (1993). A simple and efficient method for isolating RNA from pine trees. Plant Molecular Biology Reporter 11, 113–116.CrossRefGoogle Scholar
Chen, L., Song, Y., Li, S., Zhang, L., Zou, C. & Yu, D. (2012). The role of WRKY transcription factors in plant abiotic stresses. Biochimica et Biophysica Acta – Gene Regulatory Mechanisms 1819, 120–128.CrossRefGoogle ScholarPubMed
Dai, X., Xu, Y., Ma, Q., Xu, W., Wang, T., Xue, Y. & Chong, K. (2007). Overexpression of an R1R2R3 MYB gene, OsMYB3R-2, increases tolerance to freezing, drought, and salt stress in transgenic Arabidopsis. Plant Physiology 143, 1739–1751.CrossRefGoogle ScholarPubMed
Dong, P., Chen, G. Y., Liu, Y. X., Wei, Y. M., Jiang, Q. T., Li, W., Nevo, E., Liu, Y. S. & Zheng, Y. L. (2012). Molecular cloning of WRKY transcription factor sequences in wild emmer wheat (Triticum dicoccoides). African Journal of Agricultural Research 7, 6343–6349.Google Scholar
Ghahfarokhi, M. G., Mansurifar, S., Taghizadeh-Mahrjardi, R., Saeidi, M., Jamshidi, A. M. & Ghasemi, E. (2015). Effects of drought stress and rewatering on antioxidant systems and relative water content in different growth stages of maize (Zea mays L.) hybrids. Archives of Agronomy and Soil Science 61, 493–506.CrossRefGoogle Scholar
Habte, E., Müller, L. M., Shtaya, M., Davis, S. J. & von Korff, M. (2014). Osmotic stress at the barley root affects expression of circadian clock genes in the shoot. Plant, Cell & Environment 37, 1321–1327.CrossRefGoogle ScholarPubMed
Hoagland, D. R. & Arnon, D. I. (1950). The Water-culture Method for Growing Plants without Soil. California Agricultural Experiment Station Circular 347. Berkeley, CA, USA: University of California.Google Scholar
Hu, N., Tang, N., Yan, F., Bouzayen, M. & Li, Z. (2014). Effect of LeERF1 and LeERF2 overexpression in the response to salinity of young tomato (Solanum lycopersicum cv. Micro-Tom) seedlings. Acta Physiologiae Plantarum 36, 1703–1712.CrossRefGoogle Scholar
Jiang, Y., Liang, G. & Yu, D. (2012). Activated expression of WRKY57 confers drought tolerance in Arabidopsis . Molecular Plant 5, 1375–1388.CrossRefGoogle ScholarPubMed
Jiang, Q., Hu, Z., Zhang, H. & Ma, Y. (2014). Overexpression of GmDREB1 improves salt tolerance in transgenic wheat and leaf protein response to high salinity. Crop Journal 2, 120–131.CrossRefGoogle Scholar
Kharrat, M. & Ouchari, H. (2011). Faba bean status and prospects in Tunisia. Gain Legumes: the Magazine of the European Association for grain Legume Research 56, 11–12.Google Scholar
Kohan-Baghkheirati, E. & Geisler-Lee, J. (2015). Gene expression, protein function and pathways of Arabidopsis thaliana responding to silver nanoparticles in comparison to silver ions, cold, salt, drought, and heat. Nanomaterials 5, 436–467.CrossRefGoogle ScholarPubMed
Le Gall, H., Philippe, F., Domon, J. M., Gillet, F., Pelloux, J. & Rayon, C. (2015). Cell wall metabolism in response to abiotic stress. Plants 4, 112–166.CrossRefGoogle ScholarPubMed
Li, S. J., Fu, Q. T., Chen, L. G., Huang, W. D. & Yu, D. Q. (2011). Arabidopsis thaliana WRKY25, WRKY26, and WRKY33 coordinate induction of plant thermotolerance. Planta 233, 1237–1252.CrossRefGoogle ScholarPubMed
Li, J., Besseau, S., Toronen, P., Sipari, N., Kollist, H., Holm, L. & Palva, E. T. (2013). Defense-related transcription factors WRKY70 and WRKY54 modulate osmotic stress tolerance by regulating stomatal aperture in Arabidopsis. New Phytologist 200, 457–472.CrossRefGoogle ScholarPubMed
Li, C., Li, D., Shao, F. & Lu, S. (2015). Molecular cloning and expression analysis of WRKY transcription factor genes in Salvia miltiorrhiza . BMC Genomics 16, 200.CrossRefGoogle ScholarPubMed
Liu, J., He, H., Vitali, M., Visentin, I., Charnikhova, T., Haider, I., Schubert, A., Ruyter-Spira, C., Bouwmeester, H. J., Lovisolo, C. & Cardinale, F. (2015). Osmotic stress represses strigolactone biosynthesis in Lotus japonicus roots: exploring the interaction between strigolactones and ABA under abiotic stress. Planta 241, 1435–1451.CrossRefGoogle ScholarPubMed
Mingyu, Z., Zhengbin, Z., Shouyi, C., Jinsong, Z. & Hongbo, S. (2012). WRKY transcription factor superfamily: structure, origin and functions. African Journal of Biotechnology 11, 8051–8059.Google Scholar
Murray, M. G. & Thompson, W. F. (1980). Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research 8, 4321–4326.CrossRefGoogle ScholarPubMed
Nakashima, K., Fujita, Y., Katsura, K., Maruyama, K., Narusaka, Y., Seki, M., Shinozaki, K. & Yamaguchi-Shinozaki, K. (2006). Transcriptional regulation of ABI3- and ABA-responsive genes including RD29B and RD29A in seeds, germinating embryos, and seedlings of Arabidopsis . Plant Molecular Biology 60, 51–68.CrossRefGoogle ScholarPubMed
Qiu, Y. & Yu, D. Q. (2009). Over-expression of the stress-induced OsWRKY45 enhances disease resistance and drought tolerance in Arabidopsis. Environmental and Experimental Botany 65, 35–47.CrossRefGoogle Scholar
Rabara, R. C., Tripathi, P. & Rushton, P. J. (2014). The potential of transcription factor-based genetic engineering in improving crop tolerance to drought. OMICS: A Journal of Integrative Biology 18, 601–614.CrossRefGoogle ScholarPubMed
Rozen, S. & Skaletsky, H. J. (2000). Primer3 on the www for general users and for biologist programmers. In Bioinformatics Methods and Protocols (Eds Krawetz, S. & Misener, S.), pp. 365–386. Methods in Molecular Biology vol. 132. Totowa, NJ: Humana Press.Google Scholar
Rushton, P. J., Somssich, I. E., Ringler, P. & Shen, Q. J. (2010). WRKY transcription factors. Trends in Plant Science 15, 247–258.CrossRefGoogle ScholarPubMed
Scarpeci, T. E., Zanor, M. I., Mueller-Roeber, B. & Valle, E. M. (2013). Overexpression of AtWRKY30 enhances abiotic stress tolerance during early growth stages in Arabidopsis thaliana . Plant Molecular Biology 83, 265–277.CrossRefGoogle ScholarPubMed
Schluttenhofer, C., Pattanaik, S., Patra, B. & Yuan, L. (2014). Analyses of Catharanthus roseus and Arabidopsis thaliana WRKY transcription factors reveal involvement in jasmonate signaling. BMC Genomics 15, 502.CrossRefGoogle ScholarPubMed
Schmittgen, T. D. & Livak, K. J. (2008). Analyzing real-time PCR data by the comparative CT method. Nature Protocols 3, 1101–1108.CrossRefGoogle Scholar
Shen, H., Liu, C., Zhang, Y., Meng, X., Zhou, X., Chu, C. & Wang, X. (2012). OsWRKY30 is activated by MAP kinases to confer drought tolerance in rice. Plant Molecular Biology 80, 241–253.CrossRefGoogle ScholarPubMed
Siddiqui, M. H., Al-Khaishany, M. Y., Al-Qutami, M. A., Al-Whaibi, M. H., Grover, A., Ali, H. M., Al-Wahibi, M. S. & Bukhari, N. A. (2015). Response of different genotypes of faba bean plant to drought stress. International Journal of Molecular Sciences 16, 10214–10227.CrossRefGoogle ScholarPubMed
Srivastava, S. & Srivastava, M. (2014). Morphological changes and antioxidant activity of Stevia rebaudiana under water stress. American Journal of Plant Sciences 5, 3417–3422.CrossRefGoogle Scholar
Sun, X. C., Gao, Y. F., Li, H. R., Yang, S. Z. & Liu, Y. S. (2015). Over-expression of SlWRKY39 leads to enhanced resistance to multiple stress factors in tomato. The Journal of Plant Biology 58, 52–60.CrossRefGoogle Scholar
Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013). MEGA6: molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution 30, 2725–2729.CrossRefGoogle ScholarPubMed
Terzi, R., Kadioglu, A., Kalaycioglu, E. & Saglam, A. (2014). Hydrogen peroxide pretreatment induces osmotic stress tolerance by influencing osmolyte and abscisic acid levels in maize leaves. Journal of Plant Interactions 9, 559–565.CrossRefGoogle Scholar
Tuteja, N. (2007). Abscisic acid and abiotic stress signaling. Plant Signaling & Behavior 2, 135–138.CrossRefGoogle ScholarPubMed
Valifard, M., Mohsenzadeh, S. & Kholdebarin, B. (2015). Sodium chloride induced changes in photosynthetic performance and biochemical components of Salvia macrosiphon . Indian Journal of Plant Physiology 20, 79–85.CrossRefGoogle Scholar
Wang, C., Deng, P., Chen, L., Wang, X., Ma, H., Hu, W., Yao, N., Feng, Y., Chai, R., Yang, G. & He, G. (2013). A wheat WRKY transcription factor TaWRKY10 confers tolerance to multiple abiotic stresses in transgenic tobacco. PLoS ONE 8, e65120.CrossRefGoogle ScholarPubMed
Xu, X. B., Pan, Y. Y., Wang, C. L., Ying, Q. C., Song, H. M. & Wang, H. Z. (2014). Overexpression of DnWRKY11 enhanced salt and drought stress tolerance of transgenic tobacco. Biologia 69, 994–1000.CrossRefGoogle Scholar
Yan, H., Jia, H., Chen, X., Hao, L., An, H. & Guo, X. (2014). The cotton WRKY transcription factor GhWRKY17 functions in drought and salt stress in transgenic Nicotiana benthamiana through ABA signaling and the modulation of reactive oxygen species production. Plant Cell Physiology 55, 2060–2076.CrossRefGoogle ScholarPubMed
Yin, G., Xu, H., Xiao, S., Qin, Y., Li, Y., Yan, Y. & Hu, Y. (2013). The large soybean (Glycine max) WRKY TF family expanded by segmental duplication events and subsequent divergent selection among subgroups. BMC Plant Biology 13, 148. DOI: 10.1186/1471–2229–13–148 CrossRefGoogle ScholarPubMed
Yu, S., Ligang, C., Liping, Z. & Diqiu, Y. (2010). Overexpression of OsWRKY72 gene interferes in the abscisic acid signal and auxin transport pathway of Arabidopsis. Journal of Biosciences 35, 459–471.Google ScholarPubMed