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Fatty acid composition of depot fats from gnotobiotic lambs

Published online by Cambridge University Press:  27 March 2009

W. M. F. Leat ,
P. Kemp ,
R. J. Lysons  and
T. J. L. Alexander
W. M. F. Leat
Affiliation:
A.R.C. Institute of Animal Physiology, Babraham Cambridge CB2 4AT
P. Kemp
Affiliation:
A.R.C. Institute of Animal Physiology, Babraham Cambridge CB2 4AT
R. J. Lysons
Affiliation:
Department of Animal Pathology, University of Cambridge
T. J. L. Alexander
Affiliation:
Department of Animal Pathology, University of Cambridge
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Summary

The fatty acid composition of the depot fats of lambs reared for 4–5 months under gnotobiotio conditions have been determined and an estimate made of the endogenous synthesis of stearic acid.

In an uninoculated gnotobiotic lamb no hydrogenation of dietary lipids occurred in the rumen and this was reflected in the virtual absence of trans acids in depot lipids. In this animal the stearic acid content of perirenal depot fat accounted for 16% of the total fatty acids which was similar to the value found in newborn lambs (17%). Inoculation of the rumen of two gnotobiotic lambs with seven species of bacteria resulted in the establishment of partial hydrogenation processes in the rumen. Various trans isomers of octadecenoic acids were produced in these lambs from the dietary fatty acids but no stearic acid was formed.

These findings are compared with the fatty acid composition of depot fats of other herbivorous and omnivorous animals.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 88 , Issue 1 , February 1977 , pp. 175 - 179
Copyright
Copyright © Cambridge University Press 1977

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References

Alexander, T. J. L., Lysons, R. J., Elliott, L. M. & Wellstbad, P. D. (1973 a). Equipment for rearing gnotobiotio lambs. Laboratory Animals 7, 195–217.CrossRefGoogle ScholarPubMed
Alexander, T. J. L., Lysons, R. J., Elliott, L. M. & Wellstead, P. D. (1973 b). Techniques for rearing gnotobiotic lambs. Laboratory Animals 7, 239–54.CrossRefGoogle ScholarPubMed
Cook, L. J. & Reiser, R. (1968). Correlation between stearoyl desaturase activities and relative amounts of oleic and stearic acids in animal tissues. Journal of the American Oil Chemists Society 45, 488A. Abstract 80.Google Scholar
Dawson, R. M. C. & Kemp, P. (1970). Biohydrogenation of dietary fats in ruminants. In Physiology of Digestion and Metabolism in the Ruminant (ed. Phillipson, A. T.), pp. 504–18. Newcastle upon Tyne: Oriel Press.Google Scholar
Duncan, W. R. H. & Garton, G. A. (1967). The fatty acid composition and intramolecular structure of triglycerides derived from different sites in the body of the sheep. Journal of the Science of Food and Agriculture 18, 99–102.CrossRefGoogle ScholarPubMed
Duncan, W. R. H., Garton, G. A. & Matrone, G. (1971). Triglyceride fatty acids of lambs reared on a lipid-free diet. Proceedings of the Nutrition Society 30, 48A.Google Scholar
Folch, J., Lees, M. & Sloane Stanley, G. H. (1957). A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226, 497–509.Google Scholar
Garton, G. A. (1967). The digestion and absorption of lipids in ruminant animals. World Review of Nutrition and Dietetics 7, 225–50.CrossRefGoogle Scholar
Garton, G. A. & Duncan, W. R. H. (1969). Composition of adipose tissue triglycerides of neonatal and year-old lambs. Journal of the Science of Food and Agriculture 20, 39–42.CrossRefGoogle ScholarPubMed
Hilditch, T. P. & Williams, P. N. (1964). The Chemical Constitution of Natural Fats. Fourth edition. London: Chapman & Hall.Google Scholar
Kemp, P., White, R. W. & Lasder, D. J. (1975). The hydrogenation of unsaturated fatty acids by five bacterial isolates from the sheep rumen, including a new species. Journal of General Microbiology 90, 100–14.Google Scholar
Kepler, C. R., Hirons, K. P., McNeill, J. J. & Tove, S. B. (1966). Intermediates and products of the biohydrogenation of linoleic acid by Butyrivibrio fibrisolvens. Journal of Biological Chemistry 241, 1350–4.CrossRefGoogle Scholar
Leat, W. M. F. (1975). Fatty acid composition of adipose tissue of Jersey cattle during growth and development. Journal of Agricultural Science 85, 551–8.Google Scholar
Leat, W. M. F. (1976). The control of fat absorption, deposition and mobilization in farm animals. In Meat Animals: Qrowth and Productivity. New York and London: Plenum Press.Google Scholar
Leat, W. M. F., Cuthbertson, A., Howard, A. N. & Gresham, G. A. (1964). Studies on pigs reared on semisynthetio diets containing no fat, beef tallow and maize oil: composition of carcass and fatty aoid composition of various depot fats. Journal of Agricultural Science 63, 311–17.CrossRefGoogle Scholar
Leat, W. M. F., Lysons, R. J. & Alexander, T. J. L. (1973). Depot fatty acids of gnotobiotic lambs. Proceedings of the Nutrition Society 32, 97A.Google ScholarPubMed
Lysons, R. J., Alexander, T. J. L., Wellstead, P. D., Hobson, P. N., Mann, S. O. & Stewart, C. S. (1976). Studies of defined bacterial population in the rumen of gnotobiotic lambs. Journal of General Microbiology (in the Press).Google Scholar
Mortimer, C. E. & Niehaus, W. G. (1972). Enzymatic isomerization of oleic acid to trans-Δ10 octadecenoic acid. Biochemical and Biophysical Research Communications 49, 1650–6.Google Scholar
Polan, C. E., McNeill, J. J. & Tove, S. B. (1964). Biohydrogenation of unsaturated fatty acids by rumen bacteria. Journal of Bacteriology 88, 1056–64.Google Scholar
Reiser, R., Choudhury, R. B. T. & Leiohton, R. E. (1959). On the origin of stearic acid in ruminant depot fat. Journal of the American Oil Chemists Society 36, 129–30.CrossRefGoogle Scholar
Wahle, K. W. J. (1974). Desaturation of long chain fatty acids by tissue preparations of the sheep, rat and chicken. Comparative Biochemistry and Physiology 48B,87–105.Google Scholar