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The effects of fasting on the concentrations of intermediate metabolites in the blood and hepatic tissues of pregnant and non-pregnant ewes

Published online by Cambridge University Press:  27 March 2009

I. D. Herriman  and
R. J. Heitzman
I. D. Herriman
Affiliation:
Department of Functional Pathology, Agricultural Besearch Council, Institute for Research on Animal Diseases, Compton, Newbury, Berkshire RG16 0NN
R. J. Heitzman
Affiliation:
Department of Functional Pathology, Agricultural Besearch Council, Institute for Research on Animal Diseases, Compton, Newbury, Berkshire RG16 0NN
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Summary

Blood and liver samples were taken from four fasted pregnant and five fasted non-pregnant Finnish Landrace × Dorset Horn ewes. The following metabolites were measured: adenine monophosphate (AMP), adenine diphosphate (ADP), adenine triphosphate (ATP), citrate, 2-oxoglutarate, phosphoenolpyruvate, pyruvate, lactate, 2-phosphoglycerate, 3-phosphoglycerate, α-glycerophosphate, malate, β-hydroxybutyrate, acetoacetate, glutamate, alanine and NAD+.

The effects of a 6-day fast on non-pregnant ewes were shown as significant reductions in the hepatic concentrations of 2-phosphoglycerate, alanine, NAD+ and ADP, together with significant increases in the hepatic concentrations of 3-hydroxybutyrate, malate and the ratio of 3-hydroxybutyrate:acetoacetate. In the blood there were significant reductions in the concentrations of urea, citrate and in the ratio of 3-hydroxybutyrate: acetoacetate.

Similar but more marked changes occurred in the late pregnant ewes, with additional significant reductions in the hepatic concentrations of citrate, ATP and the level of cell energy, and additional increases in the blood of the ratio of lactate: pyruvate and the concentrations of 3-hydroxybutyrate and 2-oxoglutarate. However, unlike the non-pregnant ewes no significant changes in either the plasma concentration of urea or the hepatic concentration of ADP were observed in the late pregnant ewes.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 90 , Issue 3 , June 1978 , pp. 579 - 585
Copyright
Copyright © Cambridge University Press 1978

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References

Annison, E. F. & Linzell, J. L. (1964). The oxidation and utilisation of glucose and acetate by the mammary gland of the goat in relation to their over-all metabolism and milk formation. Journal of Physiology, London 175, 372–85.Google Scholar
Atkinson, D. E. (1968). Citrate and the citrate cycle in the regulation of energy metabolism. In The Metabolic Role of Citrate (ed. Goodwin, T. W.), pp. 23–40. London: Academic Press.Google Scholar
Baird, G. D. (1977). Aspects of ruminant metabolism in relation to ketosis. Biochemical Society Transactions 5, 819–27.CrossRefGoogle ScholarPubMed
Baird, G. D. & Heitzman, R. J. (1971). Mode of action of a glucocorticoid on bovine intermediary metabolism. Possible role in controlling hepatic ketogenesis. Biochimica et Biophysica Acta 252, 184–98.CrossRefGoogle ScholarPubMed
Baird, G. D., Heitzman, R. J. & Hibbitt, K. G. (1972). Effects of starvation on intermediary metabolism in the lactating cow, a comparison with metabolic changes occurring during bovine ketosis. Biochemical Journal 128, 1311–18.CrossRefGoogle ScholarPubMed
Bergman, E. N. (1963). Quantitative aspects of glucose metabolism in pregnant and non-pregnant sheep. American Journal of Physiology 204, 147–52.CrossRefGoogle Scholar
Bergman, E. N. & Hogue, D. E. (1967). Glucose turnover and oxidation rates in lactating sheep. American Journal of Physiology 213, 1378–84.Google Scholar
Farrell, D. J., Leng, R. A. & Corbett, J. L. (1972). Under-nutrition in grazing sheep. II. Calorimetrie measurements on sheep taken pasture. Australian Journal of Agricultural Research 23, 499–509.Google Scholar
Filsell, O. H., Jarrett, I. G., Taylor, P. H. & Keech, D. B. (1969). Effects of fasting, diabetes and glucocortioosteroids on gluconeogenic enzymes in sheep. Biochimica et Biophysica Acta 184, 54–63.CrossRefGoogle Scholar
Ford, E. J. H. (1963). Glucose utilisation in pregnant sheep. Biochemical Journal 88, 427–35.Google Scholar
Ford, E. J. H. (1965). The effect of diet on glucose utilisation by sheep. Journal of Agricultural Science, Cambridge 65, 41–3.Google Scholar
Heitzman, R. J., Herriman, I. D. & Mallinson, C. B. (1972). Some effects of glucocorticoids on subcellular distribution of the activities of citrate synthase and phosphoenol pyruvate carboxykinase in livers of rats and cows. FEBS Letters 20, 19–21.Google Scholar
Herriman, I. D., Heitzman, R. J., Priestley, I. & Sandhu, G. S. (1976). Concentrations of intermediate metabolites in the blood and hepatic tissues of pregnant and non-pregnant ewes. Journal of Agricultural Science, Cambridge 87, 407–11.Google Scholar
Hibbitt, K. G. (1967). Acetonaemia. Veterinary Record, suppl no. 12, iv–vi.Google Scholar
Judson, G. J. & Leng, R. A. (1973). Studies on the control of gluconeogenesis in sheep: effect of propionate, casein and butyrate infusions. British Journal of Nutrition 29, 175–95.Google Scholar
Katz, M. L. & Bergman, E. N. (1969). Hepatic and portal metabolism of glucose, free fatty acids and ketone bodies in the sheep. American Journal of Physiology 216, 953–60.CrossRefGoogle ScholarPubMed
Kronfeld, D. S. & Simesen, M. G. (1961). Glucose biokinetics in sheep. American Journal of Physiology 201, 639–44.Google Scholar
Leng, R. A. (1965). Ketone body metabolism in normal and underfed pregnant sheep and in pregnancy toxaemia. Research in Veterinary Science 6, 433–41.CrossRefGoogle ScholarPubMed
Leng, R. A. (1970 a). Glucose synthesis in ruminants. Advances in Veterinary Science and Comparative Medicine 14, 209–60.Google ScholarPubMed
Patterson, D. S. P., Burns, K. N., Cunningham, N. F., Herbert, C. N. & Saba, N. (1964). Plasma concentrations of glucose and non-esterified fatty acids (NEFA) in the pregnant and lactating ewe and the effect of dietary restriction. Journal of Agricultural Science, Cambridge 62, 253–62.CrossRefGoogle Scholar
Reid, R. L. (1968). The physiopathology of under-nourishment in pregnant sheep with particular reference to pregnancy toxaemia. Advances in Veterinary Science 12, 167–238.Google Scholar
Steel, J. W. & Leng, R. A. (1968). Effect of plane of nutrition and pregnancy on glucose entry rates in sheep. Proceedings of the Australian Society of Animal Production 7, 342–7.Google Scholar