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The effect of fungal colonization on the morphine production of poppy (Papaver somniferum L.) capsules

Published online by Cambridge University Press:  27 March 2009

J. C. Laughlin  and
D. Munro
J. C. Laughlin
Affiliation:
Department of Agriculture, Stoney Rise Centre, P.O. Box 303, Devonport, Tasmania, 7310, Australia
D. Munro
Affiliation:
New Town Research Laboratories, Department of Agriculture, P.O. Box 192B, Hobart, Tasmania, 7001, Australia
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Summary

Abnormally low concentrations of morphine in capsules of poppy (Papaver somniferum L.) in the 1970–1 season were associated with heavy fungal colonization. The effect of fungal colonization on the morphine production of capsules was later studied in a series of field, glasshouse and in vitroexperiments.

In a field experiment morphine concentration of severely colonized (> 30% surface cover) intact capsules was 20% less (P <0·01) than slightly colonized (< 10% surface cover) capsules. Colonization of these field-grown capsules was generally localized in the top half and the morphine concentration of the top half was about 20% less than the bottom half for all colonization categories. In contrast, glasshouse-grown capsules were free of fungal colonization and the top and bottom halves had similar morphine concentrations.

In a field experiment studying the effect of fungicides, 2 kg benomyl (50% a.i.) + 2 kg mancozeb (80% a.i.)/ha were applied as a spray at 10-day intervals from flowering till 1 month after commercial harvest and plants were harvested at weekly intervals from 10 days after full bloom. The mean dry-matter yield of sprayed capsules was 11% greater (P <0·01) than non-sprayed with a similar trend for morphine concentration and morphine yield. In addition, the sprayed treatment significantly reduced the area covered by sporulating lesions on the surface of the capsule after dry maturity. This superficial fungal cover had a NNE orientation in both sprayed and non-sprayed capsules.

In an in vitroexperiment using capsules from the field fungicide study, fungi were isolated from the interior of green capsule wall tissue as early as 17 days after flowering. Colonization increased with successive harvests and culturing of fungi from the interior of capsule wall tissue showed the presence of fungi in both sprayed and non-sprayed capsules with no difference in the degree of colonization.

Two of the major fungi isolated from the field experiment were identified as Dendryphion penidllatum (Corda) Fr. and Alternaria alternata (Fr.) Keissler and the individual effect of these was assessed in an in vitro experiment using ground capsule material. D. penidllatuvi and A. alternata reduced the morphine concentration of ground capsules in 24 days to 7 and 11% respectively of non-inoculated controls.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 98 , Issue 3 , June 1982 , pp. 679 - 687
Copyright
Copyright © Cambridge University Press 1982

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References

REFERENCES

Baagesgaard Rasmussen, H. & Lanng, O. (1948). Determination of morphine in poppy capsules. Method and preliminary results. Dansk Tidsskrift for Farmaci 21, 201–217 (Danish with English summary).Google Scholar
Gracza, G. & Verza-Petri, G. (1970). The effect of growth regulators on the development and alkaloid content of poppy capsules. A eta Agronomica Academia Scienlia Hungarica 19, 604–610.Google Scholar
Grummer, G. (1955). Die Beziehungen zwischen dem Eiwei Bstoffwechsel von Kulturpflanzen und ihrer Anfalligkeit gegen parasitische Pilze. Phytopathologische Zeitschrift 24 (1), 1–42.Google Scholar
Hoffman, P. J. & Menary, R. C. (1980). Variations in morphine, codeine and thebaine in the capsules of Papaver somniferum during maturation. Australian Journal of Agricultural Research 31 (2), 313–326.CrossRefGoogle Scholar
Kleinschmidt, C. & Mothes, K. (1958). Zur Zuchtung eines Arzneimohns (Papaver somniferum). Pharmazie 13, 357–360.Google Scholar
Kopp, E. (1957). Versuche zur Zuchtung einer morphinreichen Mohnsorte. Pharmazie 12, 614–620.Google Scholar
Laughlin, J. C. (1977). Nutritional and time of harvesting studies of the poppy (Papaver somniferum) on krasnozem soils of Tasmania, M.Agr.Sc. thesis, University of Tasmania.Google Scholar
Laughlin, J. C. (1978). The effect of band placed nitrogen and phosphorus fertiliser on the yield of poppies (Papaver somniferumL.) grown on krasnozem soil. Ada Horticulturae 73, 165–172.CrossRefGoogle Scholar
Laughlin, J. C. (1980). The effect of time of harvest on the yield components of poppies (Papaver somniferum L.). Journal of Agricultural Science, Cambridge 95, 667–676.CrossRefGoogle Scholar
Miczulska, L. (1967). Investigations on the effect of infestation of poppy (Papaver somniferumL.) with parasitic fungi on the content of morphine in the poppy heads. Roczniki Nauk Rolniczych 93 (A-1). 189–195 (Polish with English and Russian summaries).Google Scholar
Munro, D. (1978). Dendryphion penicillatum (Corda) Fr. on opium poppy. Australian Plant Pathology Society Newsletter 7, 8.Google Scholar
Phillipson, J. D., Handa, S. S. & El Dabbas, S. W. (1976). N-oxides of morphine, codeine and thebaine and their occurrence in Papaverspecies. Phytochemistry 15, 1297–1301.CrossRefGoogle Scholar
Pride, R. & Stern, E. S. (1954). A specific method for the determination of morphine. Journal of Pharmacy and Pharmacology 6, 590–606.CrossRefGoogle ScholarPubMed
Samborski, D. J., Forsyth, F. R. & Person, C. (1958). Metabolic changes in detached wheat leaves floated in benzimidazole and the effect of the changes in rust reaction. Canadian Journal of Botany 36, 179–182.Google Scholar
Schmitt, C. G. & Lipscomb, G. (1975). Pathogens of Selected Members of the Papaveraceae- An Annotated Bibliography. ARS-NE-62 Agricultural Research Service. U.S. Department of Agriculture.Google Scholar
Skene, K. J. (1972). Cytokinin like properties of the systemic fungicide benomyl. Journal of Horticultural Science 47, 1979–1983.Google Scholar
Wu, F. F., Dobberstein, R. H. & Morris, R. W. (1978). The effect of selected fungicides and insecticides on the growth and thebaine production of Papaver bracteatum, Lloydia 41 (4), 355–359.Google Scholar
Zambettakis, C. (1952). Reeherches sur l'helminthosporiose de l'oeillette et son traitement. Institut National de la Recherche Agronomique (Sirie c, Annales des Epiphyties) 3, 11–59.Google Scholar