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The determination of the number of bacteria in soil. (Preliminary communication)

Published online by Cambridge University Press:  27 March 2009

C. L. Whittles
C. L. Whittles
Affiliation:
School of Agriculture, Cambridge.
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Extract

1. The usual shaking methods have been examined and found unsatisfactory.

2. A method has been evolved for the estimation of the number of soil organisms by a direct count, and the numbers found to be very much higher than any previously reported.

3. A method for the disintegration and dispersion of the soil particles and bacteria has been devised, by means of which plate counts were made which were comparable with direct counts.

4. The dispersive actions of shaking and of vibrating have been compared. The former was found to give results which depended on the moisture content of the soil, whereas the trembling motion gave results in which the effect due to the moisture content was not apparent.

5. High counts made by previous observers can usually be correlated with one or more of the following factors:

(i) Greater dispersion of the soil as a result of the action of frost, etc.

(ii) An alkaline reaction, e.g. presence of ammonia, magnesia, etc.

(iii) Presence of a protective colloid, e.g. the effect of the addition of large quantities of straw, or traces of gelatine.

(iv) Absence of a flocculating agent, especially calcium compounds.

In conclusion the author wishes to express his gratitude to Dr G. S. Graham-Smith, F.R.S. at whose suggestion the investigation was started and under whose guidance and direction the bacteriological work was carried out in the Pathological Laboratory, Medical Schools, Cambridge; to Professor T. B. Wood, C.B.E., M.A., F.I.C., F.R.S., for facilities for carrying out the physico-chemical investigation at the School of Agriculture; to Messrs L. F. Newman, M.A., F.I.C., W. B. Hardy, M.A., F.R.S., W. H. Harvey, M.D., E. K. Rideal, M.B.E., M.A., G. Udny Yule, M.A., F.R.S., F. W. Foreman, M.A., F.I.C., and to the late Professor Sir German Sims Woodhead for their kindly advice and encouragement; to Messrs E. G. Staples, B.A., W. M. Davies, B.A., B.Sc, A.I.C. and A. W. R. Joachim, B.Sc. for assistance in the execution of the physico-chemical experiment; and to Mr W. A. Mitchell of the Medical School for his assistance in fitting up apparatus.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 13 , Issue 1 , January 1923 , pp. 18 - 48
Copyright
Copyright © Cambridge University Press 1923

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References

Bibliography, including a few Papers on Problems of a Similar Nature, and on the Physico-Chemical Factors Involved

(1)Abderhalden, E.Handbuch der biochemischen Arbeitsmethoden.Google Scholar
(2)Adametz, (1886). Inaug. Dissert. Leipzig. (Cited by Thiele, 1903.)Google Scholar
(3)Allen, E. R. and Bonazzi, A. (1915). On Nitrification. I. Preliminary Observations. Ohio Agr. Expt. Sta. Tech. Bul. 7.Google Scholar
(4)Allison, F. E. (1917). Biological Changes in Soils during Storage. Soil Science, 3, 37.CrossRefGoogle Scholar
(5)Arnd, Th. (1916). Beiträge zur Kenntnissder Mikrobiologie unkultivierter und kultivierter Hochmoore. Centbl. f. Bakt. Abt. II, 45, 554.Google Scholar
(6)Aumann, (1912). Über den Wert der direkten Zählung der Wasserbakterien mittels des Ultramikroskops. Centbl. f. Bakt. Abt. II, 33, 624.Google Scholar
(7)Bancroft, W. D. (1921). Peptisation and Precipitation. Report on Colloid Chemistry (Brit. Association), p. 2.Google Scholar
(8)Barthel, C. and Bengtsson, (1920). Bidrag till frágan om stallgödselkvävets nitrifikation i ákerjorden, III. Meddel. n. o. 211 fr. Centralanst. f. försöksväs pá jord bruksomrádet, Bakteriol. audel. n. o. 23. Stockholm. Abstract in Centbl. f. Bakt. Abt. II, 54, 141.Google Scholar
(9)Bartram, H. E. (1916). Effect of natural low temperature on certain fungi and bacteria. Jour. Agric. Res. 5, 651.Google Scholar
(10)Beam, W. (1911). The Mechanical Analysis of Arid Soils. Cairo Scientific Journal, 5, 107.Google Scholar
(11)Bear, F. E. (1917). A Correlation between Bacterial Activity and Lime Requirement of Soils. Soil Science, 4, 433.CrossRefGoogle Scholar
(12)Beaumont, A. B. (1919). Studies in the Reversibility of the Colloidal Condition of Soils. Cornell Univ. Agric. Exp. Sta. Mem. 21, Ann. Rep. p. 467 (Ithaca, N.Y.).Google Scholar
(13)Bemmelen, J. M. van (1910). Die Absorption. Ed. by Wo. Ostwald, pp. 117, 120, 121.Google Scholar
(14)Berkmann, M. (1913). Untersuchungen uber den Einfluss der Pflanzenwurzeln auf den Kultur des Bodens. Internal. Mitt. f. Bodenk. 3, 1.Google Scholar
(15)Beumer, (1886). Deutsche med. Wochenschr. 12, 464. (Cited by Thiele.)Google Scholar
(16)Breed, R. S. (1911). The Determination of the Number of Bacteria in Milk by direct Microscopical Examination. Centbl. f. Bakt. Abt. II, 30, 337.Google Scholar
(17)Breed, R. S. and Brew, J. D. (1916). Counting Bacteria by means of the Microscope. New York Exp. Sta. Tech. Bull. 49, 1. Abstract in Centbl. f. Bakt. Abt. II, 51, 168.Google Scholar
(18)Breed, R. S. and Dotterrer, W. D. (1916). The Number of Colonies Allowable on Satisfactory Agar Plates. Jour. Bacteriol. 1, 321.CrossRefGoogle ScholarPubMed
(19)Brew, J. D. (1914). A Comparison of the Microscopical Method and the Plate Method of Counting Bacteria in Milk. New York Exp. Sta. Bull 373. Abs. in Centbl. f. Bakt. Abt. II, 43, 250.Google Scholar
(20)Brew, J. D. (1916). The Use of 0·01 Cubic Centimeter Pipettes in Bacterial Milk Analysis. Jour. Bacteriol. 1, 89.Google Scholar
(21)Bright, J. and Conn, H. J. (1918). On the Actual Number of Bacteria in the Soil. (Proc. Soc. Amer. Bacteriologists.) Abstracts of Bacteriology, 2, 1.Google Scholar
(22)Brown, P. E. (1912). Some Bacteriological Effects of Liming. Centbl. f. Bakt. Abt. II, 34, 148.Google Scholar
(23)Brown, P. E. (1912–1913). Bacteriological Studies of Field Soils. Centbl. f. Bakt. Abt. II, 35, 234; 39, 523.Google Scholar
(24)Brown, P. E. (1913). A new Method for the Bacteriological Examination of Soils. Science, 38, 413.Google Scholar
(25)Brown, P. E. (1913). A Study of Bacteria at Different Depths in some Typical Iowa Soils. Centbl. f. Bakt. Abt. II, 37, 497.Google Scholar
(26)Brown, P. E. (1913). Media for the Quantitative Determination of Bacteria in Soils. Centbl. f. Bakt. Abt. II, 38, 497.Google Scholar
(27)Brown, P. E. and Smith, R. E. (1912). Bacterial Activities in Frozen Soils. Centbl. f. Bakt. Abt. II, 34, 369.Google Scholar
(28)Buddin, W. (1914). Partial Sterilisation of Soil by Volatile and Non-Volatile Antiseptics. Jour. Agric. Sci. 6, 417.CrossRefGoogle Scholar
(29)Buddin, W. (1914). Note on the increased Nitrate Content of a Soil subjected to Temporary Drying in the Laboratory. Jour. Agric. Sci. 6, 452.CrossRefGoogle Scholar
(30)Bühlert, and Fickendey, (1906). Zur Methodik der bakteriologischen Bodenuntersuchung. Centbl. f. Bakt. Abt. II, 16, 399.Google Scholar
(31)Cameron, F. K. (1915). Soil Colloids and Soil Solution. Jour. Phys. Chem. 19, 1.CrossRefGoogle Scholar
(32)Caron, A. (1895). Landwirtschaftlich-bakteriologische Probleme. Landw. Versuchs-Stat. 45, 401.Google Scholar
(33)Cauda, A. and Sangiorgi, G. (1914). Untersuchungen über die Mikrofauna der Böden aus Reisgegenden. Centbl. f. Bakt. Abt. II, 42, 393.Google Scholar
(34)Chambers, W. H. (1920). Studies in tho Physiology of the Fungi. XL Bacterial Inhibition by Metabolic Products. Ann. Missouri Botan. Gard. 7, 249; Abs. in Centbl. f. Bakt. Abt. II, 55, 109.CrossRefGoogle Scholar
(35)Chester, F. D. (1902). Studies in Soil Bacteriology. Del. Agr. Exp. Ann. Report, 13.Google Scholar
(36)Christensen, H. R. (1915). Studien über den Einfluss der Bodenbeschafienheit auf das Bakterienleben und den Stoffumsatz im Erdboden. Centbl. f. Bakt. Abt. II, 43, 1.Google Scholar
(37)Clayton, W. (1921). Coagulation of Inorganic Suspensions by Emulsoid. Faraday Soc. Report of Discussion on Colloids.CrossRefGoogle Scholar
(38)Comber, N. M. (1920–1921). The Flocculation of Soils. Jour. Agric. Sci. 10, 425; 11, 450.CrossRefGoogle Scholar
(39)Conn, H. J. (1909). Future Methods of Soil Bacteriological Investigation. Centbl. f. Bakt. Abt. II, 25, 454.Google Scholar
(40)Conn, H. J. (1910–1914). Bacteria of Frozen Soil. Centbl. f. Bakt. Abt. II, 28, 422; 32, 70; 42, 510.Google Scholar
(41)Conn, H. J. (1913). A Classification of the Bacteria in Two Soil Plats of Unequal Productivity. Cornell Agr. Exp. Sta. Bull. 338, 65.Google Scholar
(42)Conn, H. J. (1916). Culture Media for Use in the Plate Method of Counting Soil Bacteria. Centbl. f. Bakt. Abt. II, 44, 719.Google Scholar
(43)Conn, H. J. (1918). The Microscopic Study of Bacteria and Fungi in Soil. New York Agr. Exp. Sta. Tech. Bull. 64.Google Scholar
(44)Conn, H. J. (1921). Rose Bengal as a General Bacterial Stain. Jour. Bacteriol. 6, 253.CrossRefGoogle ScholarPubMed
(45)Conn, H. J. et al. (1921). Progress Report for 1920, Committee on Bacteriological Technique. Jour. Bacteriol. 6, 135.CrossRefGoogle Scholar
(46)Conn, H. J. and Bright, J. W. (1919). Ammonification of Manure in Soil. Jour. Agric. Res. 16, 313.Google Scholar
(47)Cook, R. C. (1916). Quantitative Media for the Estimation of Bacteria in Soils. Soil Science, 1, 153.CrossRefGoogle Scholar
(48)Coplans, M. (1912). Agglutination and Sedimentation; their Bearing on Water Storage. Jour. Path. and Bact. 17, 367.CrossRefGoogle Scholar
(49)Cunningham, A. (1915). Studies on Soil Protozoa. Jour. Agric. Sci. 7, 49.CrossRefGoogle Scholar
(50)Cutler, D. W. and Crump, L. M. (1920). Daily Periodicity in Numbers of Active Soil Flagellates…Relation of Trophic Amoebae and Bacterial Numbers. Ann. App. Biol. 7, 11.CrossRefGoogle Scholar
(51)Donnan, et al. (1917, 1920, 1921). Reports on Colloid Chemistry and its General and Industrial Application (British Association). London.Google Scholar
(52)Czermak, W. (1912). Ein Beitrag zur Erkenntnis der Veränderungen der sog. physikalischen Bodeneigenschaften durch Frost, Hitze, und die Beigabe einiger Salzen. Landw. Vers.-Stat. 76, 75.Google Scholar
(53)Eberbach, (1890). Inaug. Dissert. Dorpat. (Cited by Thiele.)Google Scholar
(54)Ehrenberg, P. (1918). Die Bodenkolloide.Google Scholar
(55)Eisenberg, P. (1918). Über Niveaubildung bei aerophilen Sporenbildnern und denitrifizierenden Bakterien. Centbl. f. Bakt. Abt. I (Orig.), 82, 209. Abs. in Centbl. f. Bakl. Abt. II, 51, 502.Google Scholar
(56)Eisenberg, P. (1919). Über Säureagglutination von Bakterien und über chemische Agglutination im allgemein. Centbl. f. Bakt. Abt. I (Orig.), 83, 70, 472, 561. Abs. in Abt. II, 51, 354.Google Scholar
(57)Elveden, , Viscount, (1921). A Contribution to the Investigation into the Results of Partial Sterilisation of the Soil by Heat. Jour. Agric. Sci. 11, 197.CrossRefGoogle Scholar
(58)Emmerich, R. (1910). Max. Pettenkofers Bodenlehre der Cholera indica. München. Abs. in Int. Mitt. f. Bodenk. 1, 483.Google Scholar
(59)Engberding, D. (1909). Vergleichende Untersuchungen über die Bakterienzahl im Ackerboden in ihrer Abhängigkeit von äusseren Einflussen Centbl. f. Bakt. Abt. II, 23, 569.Google Scholar
(60)Ernst, P. (1921). Adsorptionserscheinungen an Blutkörperchen und Bakterien. Beiträge z. patholog. Anatom. u. z. allgem. Pathol. 69, 152. Abs. in Centbl. f. Bakt. Abt. II, 55, 530.Google Scholar
(61) Fabricius and von Feilitzen (1905). Über den Gehalt an Bakterien in jungfraulichen und kultivierten Hochmoorboden auf dem Versuchsfelde des Schwedischen Moorkulturvereins bei Flahult. Centbl. f. Bakt. Abt. II, 14, 161.Google Scholar
(62)Faraday Society Discussion (1922). Physico-Chemical Problems relating to the Soil. Trans. Faraday Soc. 17.Google Scholar
(63)Fellers, C. R. (1916). Some Bacteriological Studies on Agar-Agar. Soil Science, 2, 255.CrossRefGoogle Scholar
(64)Fischer, H. (1909). Zur Methodik der Bakterienzählung. Centbl. f. Bakt. Abt. II, 25, 457.Google Scholar
(65)Fischer, H. (1909). Über den Einfluss des Kalkes auf die Bakterien eines Bodens. Landw. Vers.-Stat. 70, 335.Google Scholar
(66)Fischer, H. (1912). Vom Trocknen des Bodens. Centbl. f. Bakt. Abt. II, 36, 346.Google Scholar
(67)Fischer, H. (1921). Über die Einwirkung saurer Humusstoffe auf die biologisohen Vorgänge im Boden und im Waser. Centbl. f. Bakt. Abt. II, 54, 481.Google Scholar
(68)Fraenckel, C. (1887). Untersuchungen über das Vorkommen von Mikroorganismen in verschiedenen Bodenschichten. Zeitschr. f. Hygiene, 2, 536.Google Scholar
(69)France, R. H. (1921). Das Edaphon. Untersuchungen zur Ökologie der bodenbewohnenden Mikroorganismen. Stuttgart.Google Scholar
(70)Fred, E. B. (1916). Effect of Grinding Soil on the Number of Microorganisms. Science, 44, 282.CrossRefGoogle ScholarPubMed
(71)Friedberger, E. (1912, 1914). Über hämolytische und bakterienabtötende Wirkung chemisch indifferenter und unlöslicher anorganischer kolloidaler Substanzen. Zeitschr. f. Imrnunitätsforschung, I, 13, 127; 20, 405.Google Scholar
(72)Fries, K. A. (1921). Eine einfache Methode zur genauen Bestimmung der Bakterien mengen in Bakteriensuspensionen. Centbl. f. Bakt. Abt. I (Orig.), 86, 90.Google Scholar
(73)Fülles, (1891). Zeitschr. f. Hygiene, 10, 232. (Cited by Thiele.)Google Scholar
(74)Fulmer, H. L. (1918). Influence of Carbonates of Magnesium and Calcium on Bacteria of certain Wisconsin Soil. Jour. Agric. Res. 12, 463.Google Scholar
(75)Gainey, P. L. (1912). The Effect of Toluol and CS2 upon the Microfauna, and Fauna of the Soil. Rep. Missouri Bot. Garden, 21, 147. Abs. in Centbl. f. Bakt. Abt. II, 39, 158.CrossRefGoogle Scholar
(76)Gainey, P. L. and Gibbs, W. W. (1916). Bacteriological Studies of a Soil subjected to Different Systems of Cropping for 25 Years. Jour. Agric. Res. 6, 953.Google Scholar
(77)Gehring, A. (1918). Wie kann ich die im Boden vorhandene Zahl von Bakterien feststellen? Prometheus, 30, 93.Google Scholar
(78)Giltner, W. and Langworthy, H. V. (1916). Some Factors Influencing the Longevity of Soil Micro-organisms subjected to Desiccation, with special reference to Soil Solution. Jour. Agric. Res. 5, 927.Google Scholar
(79)Gola, G. (1910). Saggio di una teoria osmotica dell' edafismo. Ann. d. Bot. 8, 275.Google Scholar
(80)Gola, G. (1911). Osservazioni sopra i liquidi circolanti nel terreno agrario. Ann. d. R. Accad. Agric. Torino, 54.Google Scholar
(81)Goodey, T. (1915). Investigations on Protozoa in Relation to the Factor Limiting Bacterial Activity in Soil. Roy. Soc. Proc. B, 88, 437.Google Scholar
(82)Greaves, J. E. (1914). A Study of the Bacterial Activities of Virgin and Cultivated Soils. Centbl f. Bakt. Abt. II, 41, 444.Google Scholar
(83)Greaves, J. E. and Carter, E. G. (1916). Influence of Barnyard Manure upon the Bacterial Activities of the Soil. Jour. Agric. Res. 6, 889.Google Scholar
(84)Greaves, J. E. and Carter, E. G. (1920). Influence of Moisture on the Bacterial Activities of the Soil. Soil Science, 10, 361.CrossRefGoogle Scholar
(85)Greaves, J. E., Stewart, R., and Hirst, C. T. (1917). Influence of Crop, Season and Water on the Bacterial Activities of the Soil. Jour. Agric. Res. 9, 293.Google Scholar
(86)Harder, E. C. (1916). The Occurrence of Bacteria in Frozen Soil. Bot. Gaz. 61, 507.CrossRefGoogle Scholar
(87)Hardy, W. B. (1899). Preliminary Investigation of the Conditions which Determine the Stability of Irreversible Hydrosols. Proc. Roy. Soc. B, 66, 110.Google Scholar
(88)Hardy, W. B. (1900). The Mechanism of Gelation in Reversible Colloidal Systems. Jour. Phys. Chem. 4, 254.CrossRefGoogle Scholar
(89)Hardy, W. B. and Harvey, H. W. (1911). Surface Electrical Charges of Living Cells. Proc. Roy. Soc. B, 84, 217.Google Scholar
(90)Hatschek, E. (1921). Emulsion. 2nd Report on Colloid Chemistry (British Association), 2nd edition, p. 16.Google Scholar
(91)Heinze, B. (1920). Bodenbakteriologische Untersuchungen. Landw. Jahrb. 55.Google Scholar
(92)Heinze, B. (1921). Bakteriologische Versuche. Landw. Jahrb. 55, 139.Google Scholar
(93)Hilliard, C. M. and Davis, M. A. (1918). The Germicidal Action of Freezing Temperatures upon Bacteria. Journ. Bacteriol. 3, 423.CrossRefGoogle ScholarPubMed
(94)Hiltner, L. (1907). Neuere Ergebnisse und Probleme auf dem Gebiete der landwirtschaftlichen Bakteriologie. Jahresbericht V. f. angewandle Botanik, 5, 200.Google Scholar
(95)Hiltner, L. and Störmer, (1903). Studien uber die Bakterienflora des Ackerbodens mit besonderer Berücksichtigung ihres Verhaltens nach einer Behandlung mit Schwefelkohlenstoff und nach Brache. Arb. a. d. biol. Abt. f. Land- u. Forstwirth. am Kais. Ges.-Amt, 3, 445.Google Scholar
(96)Hoagland, D. R. and Martin, J. C. (1920). Effect of Season and Crop Growth on the Physical State of the Soil. Jour. Agric. Res. 20, 397.Google Scholar
(97)Hoffman, C. (1906). Relation of Soil Bacteria to Nitrogenous Decomposition. 23rd Ann. Report of Agr. Exp. Sta. Wisconsin. Abs. in Cenlbl. f. Bakt. Abt. II, 20, 343.Google Scholar
(98)Horvath, A. (1878). Über den Einfluss der Ruhe und der Bewegung auf das Leben. Pflüger's Archiv fur Physiologie, 17, 125.CrossRefGoogle Scholar
(99)HöYe, Kr. (1911). Quelques idées sur les méthodes de l'étude bactériologique des sols. La Pédologie, 13, 1. (Not seen by author.)Google Scholar
(100)Hutchinson, C. M. (1912). Studies in bacteriological Analysis of Indian Soils. Memoirs of the Dept. of Agric. in India, Bacteriol. Ser. 1, 1.Google Scholar
(101)Hutchinson, H. B. (1913). The Partial Sterilisation of the Soil by means of Caustic Lime. Jour. Agric. Sci. 5, 320.CrossRefGoogle Scholar
(102)Hutchinson, H. B. (1918). The Influence of Plant Residues on Nitrogen Fixation and on Losses of Nitrate in the Soil. Jour. Agric. Sci. 9, 92.CrossRefGoogle Scholar
(103)Hutchinson, H. B. and MacLennan, K. (1914). The Relative Effect of Lime as Oxide and Carbonate on Certain Soils. Jour. Agric. Sci. 6, 302.CrossRefGoogle Scholar
(104)Hutchinson, H. B. and Thaysen, H. C. (1918). The Non-Persistence of Bacterio-Toxins in the Soil. Jour. Agric. Sci. 9, 43.CrossRefGoogle Scholar
(105)Jones, D. H. and Murdoch, F. G. (1919). Quantitative and Qualitative Bacterial Analysis of Soil Samples Taken in the Fall of 1918. Soil Science, 8, 259.CrossRefGoogle Scholar
(106)Joseph, A. F. and Martin, F. J. (1921). The Determination of Clay in Heavy Soils. Jour. Agric. Sci. 11, 293.CrossRefGoogle Scholar
(107)Keith, S. C. (1913). Factors influencing the Survival of Bacteria at Temperatures in the Vicinity of the Freezing Point of Water. Science, New Series, 37, 877.CrossRefGoogle ScholarPubMed
(108)Keller, R. (1921). Elektromikroskopie. Naturw. Wochenschr. N. Folg. 20, 665.Google Scholar
(109)Kellerman, K. F. and Allen, P. W. (1911). Bacteriological Studies of the Soils of the Truckee-Carson Irrigation Project. U.S. Dept. Agric. Bur. of Plant Indus. Bull. 211. Abs. in Centbl. f. Bakt. Abt. II, 33, 374.Google Scholar
(110)King, W. E. and Doryland, C. J. T. (1909). The Influence of Cultivation upon Soil Bacteria and their Activities. Kansas Exp. Sta. Bull. 161. Abs. in Centbl. f. Bakt. Abt. II, 30, 268.Google Scholar
(111)Koch, A. (1918). Bodenbakterien und Pflanzennährung. Jahrb. d. Deutsch. Landw. Gesellsch. 33, 67. Abs. in Centbl. f. Bakt. Abt. II, 50, 177.Google Scholar
(112)Kossowicz, A. (1912). Einführung in die Agrikulturmycologie. I Teil: Bodenbakteriologie. Berlin. (Not seen by author.)Google Scholar
(113)Krüger, W. and Heinze, B. (1907). Untersuchungen über das Wesen der Brache Landw. Jahrb. 36, 383.Google Scholar
(114)Lafar, F. (1910). Technical Mycology, 1, 63.Google Scholar
(115)Lantzsch, K. (1921). Bacillus amylobacter A. et Bred, und seine Beziehung zu den Kolloiden. Centbl. f. Bakt. Abt. II, 54, 1.Google Scholar
(116)Le Clair, C. A. (1915). Influence of Growth of Cowpeas upon some Physical, Chemical and Biological Properties of Soil. Jour. Agr Res. 5, 439.Google Scholar
(117)Lemmermann, O., Einecke, , and Fischer, H. (1911). Untersuchungen über die Wirkung eines verschiedenen Verhältnisses von Kalk und Magnesia in einigen Böden aufhöhere Pflanzen und Mikroorganismen. Landw. Jahrb. 40, 173.Google Scholar
(118)Lemmermann, O., Fischer, H., Kappen, H. and Blenck, E. (1909). Bakteriologischchemische Untersuchungen. Landw. Jahrb. 38, 319.Google Scholar
(119)Liesegang, R. (1912). Wachsende Kieselsäuregele. Ztschr. Koll. 10, 273.CrossRefGoogle Scholar
(120)Lint, H. C. and Coleman, D. A. (1916). Sources of Error in Soil Bacteriological Analysis. Soil Science, 2, 157.CrossRefGoogle Scholar
(121)Lipman, C. B. (1912). The Distribution and Activities of Bacteria in Soils of the Arid Region. Univ. of California Pub. in Agric. Sci. 1, 1.Google Scholar
(122)Lipman, C. B. (1912). Toxic Effects of Alkali Salts in Soils on Soil Bacteria. Centbl. f. Bakt. Abt. II, 32, 58; 33, 305.Google Scholar
(123)Lipman, C. B. (1914). Antagonism between Anions as related to Nitrogen Transformations in Soils. The Plant World, 17, 295.Google Scholar
(124)Lipman, C. B. and Burgess, P. S. (1914). The Protective Action against MgCO3 of CaCO8 for A. chroococcum. Jour. Agric. Sci. 6, 484.CrossRefGoogle Scholar
(125)Lipman, C. B. and Burgess, P. S. (1914). Antagonism between Anions as affecting Soil Bacteria. Centbl. f. Bakt. Abt. II, 36, 382; 41, 430; 42, 502.Google Scholar
(126)Lipman, C. B. and Martin, D. E. (1918). Are Unusual Precautions Necessary in Taking Soil Samples for Ordinary Bacteriological Tests? Soil Science, 6, 131.CrossRefGoogle Scholar
(127)Lipman, J. G. (1916). Problems in Soil Bacteriology. Proc. Amer. Soc. Bacteriologists. Abs. in Centbl. f. Bakt. Abt. II, 45, 376.Google Scholar
(128)Lipman, J. G. and Brown, P. E. (1909). Media for the Quantitative Estimation of Soil Bacteria. Centbl. f. Bakt. Abt. II, 25, 447.Google Scholar
(129)Lipman, J. G. and Owen, I. L. (1910). Some Bacteriological Relations in Soils kept under Green-house Conditions. Jour. Agric. Sci. 3, 301.CrossRefGoogle Scholar
(130)Loeb, J. (1915). The Mechanism of Antagonistic Salt Action. Proc. Nat. Acad. Sci. Washington, 1, 473.CrossRefGoogle ScholarPubMed
(131)Löhnis, F. (1904). Beitrag zur Methodik der bakteriologischen Bodenuntersuchung. Centbl. f. Bakt. Abt. II, 12, 262.Google Scholar
(132)Löhnis, F. (1905). Zur Methodik der bakteriologischen Bodenuntersuchung. Centbl. f. Bakt. Abt. II, 14, 1.Google Scholar
(133)Löhnis, F. (1912). Ziele und Wege der bakteriologischen Bodenforschung. Landw. Jahrb. 42, 751.Google Scholar
(134)Löhnis, F. (1914). Bodenbakterien und Bodenfruchtbarkeit. Berlin. Abs. in Centbl. f. Bakt. Abt. II, 43, 473.Google Scholar
(135)Löhnis, F. (1921). Ergebnisse amerikanischer, britischer und französischer Arbeiten auf dem Gebiete der landwirtschaftlichen Bakteriologie aus den Jahren 1915–1920. Centbl. f. Bakt. Abt. II, 54; 273.Google Scholar
(136)MacIntire, W. H. (1914). Decomposition of Soil Carbonates. Jour. Agric. Res. 3, 79.Google Scholar
(137)Makkus, W. (1914). Die Brache, ihre Physiologie, Formen, Zweck, Bedeutung, und Vebreitung, einst und jetzt. Eine kritische Studie. Landw. Jahrb. 47, 673.Google Scholar
(138)Mayer, W. (1920). Die Veröffentlichungen der amerikanischen Moorkulturgesellschaft im Jahre 1917. Mitt. d. Ver. z. Förder. d. Moorkultur i. Deutsch. Reicht, Jahrg. 38, 80, 206, 219, 230. Abs. in Centbl. f. Bakt. Abt. II, 55, 338.Google Scholar
(139)Meltzer, S. J. (1894). Über die fundamental Bedeutung der Erschütterung für die lebende Materie. Zeitschr. f. Biologie, 30, 464. Abs. in Centbl. f. Bakt. 16, 743.Google Scholar
(140)Micheels, H. (1907). Sur l'eau distillée et le liquide physiologique. Archiv. internal. de Physiol. 4, 415.Google Scholar
(141)Migula, W. (1913). Über die Tätigkeit der Bakterien im Waldboden. Forstwissensch. Zentralbl. 35, 161.CrossRefGoogle Scholar
(142)Millard, W. A. (1912). Bacteriological Tests in Soil and Dung. Centbl. f. Bakt. Abt. II, 31, 502.Google Scholar
(143)Miquel, (1882). Annuaire de l'observatoire de Montsouris pour l'an 1882.Google Scholar
(144)Mittelbach, H. (1921). Über die desinfizierende Wirkung der Kupfersalze. Centbl. f. Bakt. Abt. I (Orig.), 86, 44. Abs. in Abt. II, 55, 108.Google Scholar
(145)Morgan, J. F. (1916). The Soil Solution as an Index of the Biological Changes in the Soil. Jour. Bacteriol. 1, 97.Google Scholar
(146)Morgan, J. F. (1917). The Soil Solution obtained by the Oil Pressure Method. Soil Science, 3, 531.CrossRefGoogle Scholar
(147)Morgan, J. F. and Gruzit, O. M. (1916). Reaction of the Soil Solution as an Index of Biological Changes in the Soil. Jour. Bacteriol. 1, 97.Google Scholar
(148)Müller, P. Th. (1912). Über die Rolle der Protozoen bei der Selbstreinigung stehenden Wassers. Arch. f. Hygiene, 75, 321.Google Scholar
(149)Müntz, A. and Gaudechon, H. (1912). Le Reveil de Terre. Compt. Rend. hebd. de l'Ac. Paris, 154, 163.Google Scholar
(150)Murawijansky, S. M. (1913). Ultramikroskopie von Bodenauszügen. Pedologie (2/3), 23. Abs. in Int. Mitt. f. Bodenk. 4, 78.Google Scholar
(151)Murray, T. J. (1921). The Effect of Straw on the Biological Soil Processes. Soil Science, 12, 233.CrossRefGoogle Scholar
(152)Neller, J. R. (1917). A Soil Sampler for Bacteriological and Chemical Purposes. Soil Science, 4, 109.CrossRefGoogle Scholar
(153)Niklas, H. (1913). Die Kolloidchemie und ihre Bedeutung für Bodenkunde, Geologie und Mineralogie. Int. Mitt. f. Bodenk. 3, 383.Google Scholar
(154)Nolte, O. and Hahn, E. (1917). Action of Frost on Soil. Jour. Landw. 65, 75. (Original not seen.)Google Scholar
(155)Noyes, H. A. (1918). Bacteria in Frozen Soil. Proc. Ind. Acad. Sci. p. 110. (Original not seen.)Google Scholar
(156)Noyes, H. A. and Conner, S. D. (1919). Nitrates, Nitrification and Bacterial Contents of Five Typical Acid Soils as Affected by Lime, Fertiliser, Crops and Moisture. Jour. Agric. Res. 16, 27.Google Scholar
(157)Noyes, H. A. and Grounds, G. L. (1918). Number of colonies for a satisfactory soil plate. Proc. Ind. Acad. Sci. p. 93. (Original not seen.)Google Scholar
(158)Noyes, H. A. and Voigt, E. (1917). A Technic for the Bacteriological Examination of Soil. Proc. Ind. Acad. Sci. p. 272. Abs. of Bact. 2, 3, 131. (Original not seen.)Google Scholar
(159)Noyes, H. A., Voigt, E., and Luckett, (1918). The Length of Time to Incubate a Petri Plate. Proc. Ind. Acad. Sci. p. 102. (Original not seen.)Google Scholar
(160)Oehler, R. (1911). Über Joghartkontrolle. Centbl. f. Bakt. Abt. II, 30, 149.Google Scholar
(161)Olaru, D. A. (1920). Rôle du manganèse en agriculture. Son influence sur quelques microbes du sol. Paris. Abs. in Centbl. f. Bakt. Abt. II, 54, 145. (Original not seen.)Google Scholar
(162)Penfold, W. J. and Ledingham, J. C. G. (1914). On the Nature of Bacterial Lag. Mathematical analysis of the lag phase in bacterial growth. Jour. Hygiene, 14, 215.CrossRefGoogle Scholar
(163)Percival, J. and Mason, G. H. (1913). The Micro-flora of Stilton Cheese. [A description that might well apply to that of a soil.] Jour. Agric. Sci. 5, 222.CrossRefGoogle Scholar
(164)Perfiliev, B. (1913). Ein Schlammsauger zur Gewinnung der Bodenmikroflora und -fauna. [Russian with German résumé.] Bull. d.jard. impér. bolan. de St Pétersbourg. 13, 45. Abs. in Centbl. f. Bakt. Abt. II, 41, 312. (Original not seen.)Google Scholar
(165)Perotti, R. (1911). Sopra la microflora della Campagna romana. Rendic. Accad. Lincei, Ser. 5, 20, 690. Abs. in Centbl. f. Bakt. Abt. II, 41, 275. (Original not seen.)Google Scholar
(166)Prausnik, W. (1911). Die Hygiene des Bodens. Handbuch der Hygiene (Rubner usw.), 1, 520. Abs. in Int. Mitt. f. Bodenk. 4, 239.Google Scholar
(167)Prescott, J. A. (1916). The Phenomenon of Absorption in its relation to Soils. A Résumé of the Subject. Jour. Agric. Sci. 8, 111.CrossRefGoogle Scholar
(168)Prescott, J. A. (1920). A Note on the Sheraqi Soils of Egypt. A Study in Partial Sterilisation. Jour. Agric. Sci. 10, 177.CrossRefGoogle Scholar
(169)Pringsheim, E. G. (1920). Über die gegenseitige Schädigung und Förderung von Bakterien. Centbl. f. Bakt. Abt. II, 51, 72.Google Scholar
(170)Rahn, O. (1906). Bakteriologische Untersuchungen über das Trocknen des Bodens. Centbl. f. Bakt. Abt. II, 20, 38.Google Scholar
(171)Rahn, O. (1913). Die Bakterientätigkeit im Boden als Funktion der Nahrungskonzentration und der unlöslichen organischen Substanz. Centbl. f. Bakt. Abt. II, 38, 484.Google Scholar
(172)Rahn, O. (1913). Bacterial Activity in Soil as a Function of the various physical Soil Properties. Science, 38, 414.Google Scholar
(173)Reimers, (1889). Zeitschr. f. Hygiene, 7, 315. (Cited by Thiele.)Google Scholar
(174)Reinke, J. (1880). Über den Einfluss mechanischer Erschütterung auf die Entwicklung der Spaltpilze. Pflüger's Archiv f. Physiol. 23, 434.CrossRefGoogle Scholar
(175)Remy, T. (1902). Bodenbakteriologische Studien. Die Bakterienzahl als Kennzeichen des Fruchtbarkeitsfcustandes eines Bodens. Centbl. f. Bakt. Abt. II, 8, 732.Google Scholar
(176)Remy, T. and Rösing, G. (1911). Beitrag zur Methodik der bakteriellen Bodenuntersuchung. Centbl. f. Bakt. Abt. II, 29, 36.Google Scholar
(177)Remy, T. and Rösing, G. (1911). Über die biologische Reizwirkung natürlicher Humusstoffe. Centbl. f. Bakt. Abt. II, 30, 349.Google Scholar
(178)Rippert, (1920). Die Nutzbarmachung der Moore zur Bodenverbesserung und zur Düngung der Kulturpflanzen. Mitt. d. Ver. z. Förder. d. Moorkult. i. Deutsche Reiche, 38, 30. Abs. in Centbl. f. Bakt. Abt. II, 55, 338. (Original not seen.)Google Scholar
(179)Ritter, G. A. (1912). Das Trocknen der Erden. Centbl. f. Bakt. Abt. II, 33, 116.Google Scholar
(180)Rivas, D. (1910). Bacteria and other Fungi in relation to the Soil. Contr. Bot. Laborat. Univ. Pennsylvania, 3 (3), 243. Abs. in Centbl. f. Bakt. Abt. II, 30, 72.Google Scholar
(181)Rohland, P. (1912). Über die Adsorptionsfähigkeit der Hydroxyde des Siliciums, Aluminiums und Eisens. Zeitschr. f. anorganische Chemie, p. 74. Abs. in Int. Mitt, f. Bodenk. 3, 482. (Original not seen.)CrossRefGoogle Scholar
(182)Rohland, P. (1913). Die Kolloidstoffe in den Tonen und Böden. Int. Mitt. f. Bodenk, 3, 487Google Scholar
(183)Rohland, P. (1914). Die Wirkung der Hydroxylionen auf Tone und tonige Böden. Landw. Versuchs-Stat. 85, 106.Google Scholar
(184)Rohland, P. (1914). Die Bodenkolloide und ihre Adsorptionsfähigkeit. Landw. Jahrb. 47, 239.Google Scholar
(185)Rohland, P. (1914). Die Adsorptionsfähigkeit der Böden. Int. Mitt. f. Bodenk. 4, 393.Google Scholar
(186)Rosenkranz, H. (1920). Untersuchungen über die praktische Verwertbarkeit der oligodynamischen Wirkung der Kupfersalze auf Bakterien. (Inaug. Diss.) München. Abs. in Centbl. f. Bakt. Abt. II, 55, 108.Google Scholar
(187)Rossi, G. (1921). Preliminary Note on the Microbiology of the Soil and the Possible Existence therein of Invisible Germs. Soil Science, 12, 409.CrossRefGoogle Scholar
(188)Ruata, G. Q. (1903). Quantitative Analyse bei der bakteriologische Diagnose der Wässer. Centbl. f. Bakt. Abs. II, 11, 220. (N.B. p. 287.)Google Scholar
(189)Russell, E. J. (1915). Soil Protozoa and Soil Bacteria. Proc. Roy. Soc. Lond. B, 89, 76.Google Scholar
(190)Russell, E. J. (1921). Soil Conditions and Plant Growth. (4th edition.) Pp. 239, 265.Google Scholar
(191)Russell, E. J. (1921). Report on the Part Played by Colloids in Agricultural Phenomena. 2nd Report on Colloid Chemistry (Brit. Assoc), p. 70.Google Scholar
(192)Russell, E. J. and Goulding, J. (1912). Investigations on “Sickness” in Soil. I. Sewage Sickness. Jour. Agric. Sci. 5, 27.CrossRefGoogle Scholar
(193)Russell, E. J. and Hutchinson, H. B. (1909, 1913). The Effect of Partial Sterilisation of Soil on the Production of Plant Food. Jour. Agric. Sci. 3, 111; 5, 152.CrossRefGoogle Scholar
(194)Russell, E. J. and Petherbridge, F. R. (1912). Investigations on “Sickness” in Soil. II. “Sickness” in Glass-house Soils. Jour. Agric. Sci. 5, 86.CrossRefGoogle Scholar
(195)Russell, H. L. (1892). The Effect of Mechanical Movement upon the Growth of Certain Lower Organisms. Botan. Gaz. No. 1 (Bloomington, Indiana).CrossRefGoogle Scholar
(196)Schmelck, (1888). Steigerung des Bakteriengehaltes im Wasser während des Schneesmelzens. Centbl. f. Bakt. Abt. II, 4, 195.Google Scholar
(197)Schulz, K. (1913). Die Verbreitung der Bakterien im Waldboden. (Inaug. Diss.) Jena. Abs. in Centbl. f. Bakt. Abt. II, 41, 277. (Original not seen.)Google Scholar
(198)Schwarz, E. H. L. (1909). The Organisms of the Soil. Science Progress, 4 (13), 150.Google Scholar
(199)Severin, S. A. (1909). Über die Bakterienflora einiger Bodenportionen aus dem fernen Norden (Obdorsk und Halbinsel Jamal). Centbl. f. Bakt. Abt. II, 25, 470.Google Scholar
(200)Sharp, L. F. (1913). Some bacteriologic Studies of old Soils. Plant World, 16, 101. Abs. in Centbl. f. Bakt. Abt. II, 40, 193.Google Scholar
(201)Skar, O. (1912). Eine schnelle und genaue Methode f¨r Zahlung von Bakterien und Leukocyten. Milch Zentralbl. (15 and 23). Abs. in Centbl. f. Bakt. Abt. II, 37, 329.Google Scholar
(202)Söhngen, N. L. (1913). Einfluss von Kolloiden auf mikrobiologische Prozesse. Centbl. f. Bakt. Abt. II, 38, 621.Google Scholar
(203)Szücs, J. (1912). Experimented Beiträge zu einer Theorie der antagonistischen Ionenwirkung. Jahrb. f. wiss. Bot. 52, 85. Abs. in Centbl. f. Bakt. Abt. II, 43, 523 (Original not seen.)Google Scholar
(204)Temple, J. C. (1912). The Influence of Stall Manure upon the Bacterial Flora of the Soil. Centbl. f. Bakt. Abt. II, 34, 204.Google Scholar
(205)Thaer, W. (1910). Der Einfluss von Kalk und Humus auf die mechanische, physikalische und chemische Beschaffenheit von Ton-, Lehm-, und Sand-boden. (Gekrönte Preisschr.) Göttingen. Abs. in Centbl.f. Bakt. Abt. 11, 32, 271. (Original not seen.)Google Scholar
(206)Thiele, R. (1903). Beiträge zur Methodik der Bodensforschung. I. Die Bestimmung der Zahl der Mikroorganismen im Boden. Centbl. f. Bakt. Abt. II, 11, 251.Google Scholar
(207)Thornton, H. G. (1922). On the Development of a Standardised Agar Medium for counting soil bacteria. Ann. Appl. Biol. 9, 241.CrossRefGoogle Scholar
(208)Thornton, W. M. (1912). The Electrical Conductivity of Bacteria and the Rate of Sterilisation of Bacteria by Electric Currents. Proc. Roy. Soc. Lond. B, 85, 331.Google Scholar
(209)Truffaut, G. and Bezssonoff, H. (1920). Influence de la Stérilisation partielle sur la composition de la flore microbienne du sol. Compt. rend, hebdom. séance Acad. d. science, Paris, 170, 1278.Google Scholar
(210)Vass, A. F. (1919). The Influence of Low Temperature on Soil Bacteria. Cornell Univ. Agr. Exp. Sta. (Mem. 27), Ann. Report, p. 1039. (Ithaca, New York.)Google Scholar
(211)Vogel, (1910). Beiträge zur Methodik der bakteriologischen Bodenuntersuchung. Mitt. d. Kaiser Wilhelms Inst. f. Landw. in Bromberg, 2 (4). Abs. in Centbl. f. Bakt. Abt. II, 27, 593.Google Scholar
(212)Waal, J. J. De (1920). Zur bakteriologischen Untersuchung des Trinkwassers Centbl. f. Bakt. Abt. II, 52, 10.Google Scholar
(213)Waite, H. H. and Squires, D. H. (1911). A Comparative Study of the Bacterial Content of Soils from Fields of Com and Alfalfa. Ann. Rep. Nebraska Agric. Exp. Sta. 24, 160. Abs. in Centbl. f. Bakt. Abt. II, 33, 375. (Original not seen.)Google Scholar
(214)Waksman, S. A. (1916). Bacterial Numbers in Soils at Different Depths and in Different Seasons of the Year. Soil Science, 1, 363.CrossRefGoogle Scholar
(215)Weber, G. G. A. (1912). Die Einwirkung der Kälte auf die Mikroorganismen und ihre Tätigkeit im Boden. (Diss. phil.) Jena. Abs. in Centbl. f. Bakt. Abt. 11, 37, 113. (Original not seen.)Google Scholar
(216)Weeter, H. M. (1916). Study of Effect of Dilution Water on Bacterial Suspensions. Jour. Bacteriol. 1, 92.Google Scholar
(217)Weiss, A. (1920). Zur Bestimmung der Keimzahl im Wasser. Centbl.f.Bakt. Abt. II, 52, 18.Google Scholar
(218)Wiegner, G. (1921). Boden und Bodenbildung in kolloidchemischer Betrachtung. Dresden and Leipzig.Google Scholar
(219)Williams, B. (1916). Some Factors influencing Nitrogen Fixation and Nitrification. Botan. Gaz. 62, 311. Abs. in Centbl. f. Bakt. Abt. II, 54, 139. (Original not seen.)CrossRefGoogle Scholar
(220)Wojtkiewicz, A. (1914). Beiträge zu bakteriologischen Boden-Untersuchungen. Centbl. f. Bakt. Abt. II, 42, 254.Google Scholar
(221)Wolkoff, M. I. (1916–1917). Studies on Soil Colloids. Soil Science, 1, 585; 3, 423.CrossRefGoogle Scholar
(222)Wood, T. B. and Stratton, F. J. M. (1910). The Interpretation of Experimental Results. Jour. Agric. Sci. 3, 417.CrossRefGoogle Scholar
(223)Wyant, Z. N. (1921). A Comparison of the Technic Recommended by Various Authors for Quantitative Bacteriological Analysis of Soil. Soil Science, 11, 295.CrossRefGoogle Scholar
(224)Zeug, M. (1920). Äquilibrierte Salzlösungen als indifferente Suspensionsflüssigkeiten für Bakterien. Arch.f. Hyg. 89, 175. Abs. in Centbl. f. Bakt. Abt. II, 55, 527.Google Scholar
(225)Zsigmondy, R. and Spear, E. B. (1917). The Chemistry of Colloids. New York. (p. 151.)Google Scholar