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A cranial mesenteric vein preparation for measurement of amino acid uptake by lambs

Published online by Cambridge University Press:  27 March 2009

S. A. Neutze ,
V. H. Oddy  and
J. M. Gooden
S. A. Neutze
Affiliation:
Department of Animal Science, University of Sydney, Camden, NSW 2570, Australia
V. H. Oddy
Affiliation:
Elizabeth MacArthur Agricultural Institute, NSW Agriculture, PMB 8, Camden, NSW 2570, Australia
J. M. Gooden
Affiliation:
Department of Animal Science, University of Sydney, Camden, NSW 2570, Australia
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Summary

A surgical preparation was developed to measure uptake of amino acids from the small intestine into the cranial mesenteric vein (CMV) of lambs. Results from this preparation were compared with those from the traditional hepatic portal vein (PV) preparation in the same lambs. Necropsy revealed that, in contrast to the PV preparation which included all portal-drained viscera, the CMV preparation drained primarily small intestine (0·84–0·92 small intestinal mass). The CMV preparation contributed 0·238 of blood flow from and 0·307 of oxygen consumption by the PV preparation. Venous-arterial differences for alpha-amino nitrogen (AAN), phenylalanine (Phe) and tyrosine (Tyr) were three- to fourfold greater in the CMV than in the PV preparation, indicating that the CMV preparation is more sensitive for measuring amino acid uptake. The ratio CMV/PV for uptake of AAN was 0·61 (P< 0·001), but this ratio was much closer to unity (mean 0·84) for Phe and Tyr. Since the CMV preparation drains primarily the small intestine, through which all amino acid absorption occurs, it should prove to be extremely valuable for studying absorption from and metabolism within this organ using a combination of arteriovenous and isotopic tracer techniques.

Type
Animals
Information
The Journal of Agricultural Science , Volume 122 , Issue 2 , April 1994 , pp. 309 - 314
Copyright
Copyright © Cambridge University Press 1994

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References

REFERENCES

Barnes, R. J., Comline, R. S. & Dobson, A. (1983). Changes in the blood flow to the digestive organs of sheep induced by feeding. Quarterly Journal of Experimental Physiology 68, 77–88.Google Scholar
Ben-Ghedalia, D., Tagari, H., Bondi, A. & Tadmor, A. (1974). Protein digestion in the intestine of sheep. British Journal of Nutrition 31, 125–142.Google Scholar
Getty, R. (1975). Sisson and Grossman's The Anatomy of the Domestic Animals, 5th Edn, pp. 983–985. Philadelphia: W. B. Saunders Company.Google Scholar
Harris, P. M., Waghorn, G. C.& Lee, J. (1990). Nutritional partitioning of growth for productive gain. Proceedings of the New Zealand Society of Animal Production 50, 81–90.Google Scholar
Huntington, G. B. (1987). Net absorption from portaldrained viscera of nitrogenous compounds by beef heifers fed on diets differing in protein solubility or degradability in the rumen. British Journal of Nutrition 57, 109–114.Google Scholar
Katz, M. L. & Bergman, E. N. (1969). A method for simultaneous cannulation of the major splanchnic blood vessels of the sheep. American Journal of Veterinary Research 30, 655–661.Google Scholar
Lee, H. M., Forde, M. D., Lee, M. C. & Bucher, D. J. (1979). Fluorometric microbore amino acid analyzer: the construction of an inexpensive, highly sensitive instrument using o-phthalaldehyde as a detection agent. Analytical Biochemistry 96, 298–307.Google Scholar
MacRae, J. C. & Reeds, P. J. (1980). Prediction of protein deposition in ruminants. In Protein Deposition in Animals (Eds Buttery, P. J. & Lindsay, D. B.), pp. 225–249. London: Butterworths.Google Scholar
Neutze, S. A., Oddy, V. H., Gooden, J. M., Forbes, W. A. & Warren, H. M. (1990). Portal uptake of amino acids by sheep given oaten chaff supplemented with rumen escape protein. Proceedings of the Nutrition Society of Australia 15, 145.Google Scholar
Oddy, V. H. (1974). A semi-automated method for the determination of plasma alpha amino nitrogen. Clinical Chimica Ada 51, 151–156.Google Scholar
Oddy, V. H. & Lindsay, D. B. (1986). Determination of rates of protein synthesis, gain and degradation in intact hind-limb muscle of lambs. Biochemical Journal 233, 417–425.Google Scholar
Oddy, V. H., Gooden, J. M. & Annison, E. F. (1984). Partitioning of nutrients in Merino ewes. I. Contribution of skeletal muscle, the pregnant uterus and the lactating mammary gland to total energy expenditure. Australian Journal of Biological Sciences 37, 375–388.Google Scholar
Prior, R. L., Huntington, G. B. & Britton, R. A. (1981). Influence of diet on amino acid absorption in beef cattle and sheep. Journal of Nutrition 111, 2212–2222.Google Scholar
Reynolds, C. K. & Huntington, G. B. (1988). Partition of portal-drained viscera net flux in beef steers. 1. Blood flow and net flux of oxygen, glucose and nitrogenous compounds across stomach and post-stomach tissues. British Journal of Nutrition 60, 539–551.Google Scholar
Seal, C. J., Parker, D. S. & Avery, P. J. (1992). The effect of forage and forage-concentrate diets on rumen fermentation and metabolism of nutrients by the mesentericand portal-drained viscera in growing steers. British Journal of Nutrition 67, 355–370.Google Scholar
Tagari, H. & Bergman, E. N. (1978). Intestinal disappearance and portal blood appearance of amino acids in sheep. Journal of Nutrition 108, 790–803.Google Scholar
Wolff, J. E., Bergman, E. N. & Williams, H. H. (1972). Net metabolism of plasma amino acids by liver and portal-drained viscera of fed sheep. American Journal of Physiology 223, 438–446.Google Scholar