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The application of an improved artificial rumen technique to the study of the effects of non-protein nitrogen on fermentation and nitrogen distribution in the rumen

Published online by Cambridge University Press:  27 March 2009

T. W. Griffiths
T. W. Griffiths
Affiliation:
Spillers Ltd., Animal Nutrition Research Laboratory, Middle Aston House, Steeple Aston, Oxford
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Summary

An artificial rumen technique which simulated in vivo conditions was applied to the study of the effects of non-protein nitrogen on fermentation and nitrogen distribution in the rumen. Using this technique it was possible to reproduce in vitro a series of biochemical and other effects on fermentation that had been produced in vivo by the addition of various sources and levels of non-protein nitrogen to the rumen. It was shown that with a basal diet of cellulose, protein was synthesized from urea both in the presence and absence of starch over a period of 24 h and a typical rumen fermentation pattern was maintained.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 71 , Issue 1 , August 1968 , pp. 105 - 112
Copyright
Copyright © Cambridge University Press 1968

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References

Balch, C. C. & Campling, R. C. (1961). Utilization of urea by milking cows. J. Dairy Res. 28, 157–63.CrossRefGoogle Scholar
Belasco, I. J. (1956). The role of carbohydrates in urea utilization, cellulose digestion and fatty acid formation. J. Anim. Sci. 15, 496–508.CrossRefGoogle Scholar
Caffrey, P. J., Hatfield, E. E., Norton, W. H. & Garrigus, U. S. (1967). Nitrogen metabolism in the bovine. I. Adjustment to a urea rich diet. J. anim. Sci. 26, 595–600.CrossRefGoogle Scholar
Chalupa, W., Evans, J. L. & Stillions, M. C. (1964). Metabolic aspects of urea utilization by ruminant animals. J. Nutr. 84, 77–82.Google Scholar
Conway, E. J. (1957). Microdiffusion Analysis and Volumetric Error. London: Crosby Lockwood.Google Scholar
Ewan, R. C., Hatfield, E. E. & Garrigus, U. S. (1958). The effect of certain inoculations on the utilization of urea or biuret by growing lambs. J. anim. Sci. 17, 298–303.CrossRefGoogle Scholar
Griffiths, T. W. (1967). The evaluation of an improved artificial rumen technique for the study of rumen fermentation. J. agric. Sci., Camb. 69, 355–66.Google Scholar
Hemsley, J. A. (1966). Dietary cellulose content and urea utilization. Proc. Aust. Soc. Anim. Prod. 6, 384–8.Google Scholar
Hungate, R. E. (1966). In The Rumen and Its Microbes, p. 303. London: Academic Press.Google Scholar
Jayasinghe, J. B. (1958). The digestion of protein in the ruminant with special reference to milk production. Ph.D. thesis, University of Aberdeen.Google Scholar
Johnson, R. R. & McClure, K. E. (1964). In vitro and in vivo comparisons on the utilization of urea, biuret and diammonium phosphate by sheep. J. anim. Sci. 23, 208–13.Google Scholar
Lewis, D., Hill, K. J. & Annison, E. F. (1957). Studies on the portal blood of sheep. I. Absorption of ammonia from the rumen of the sheep. Biochem. J. 66, 587–92.CrossRefGoogle ScholarPubMed
McLaren, G. A., Anderson, G. C, Tsai, L. I. & Barth, K. M. (1965). Level of readily available carbohydrates and adaption of lambs to all urea supplemented rations. J. Nutr. 87, 331–6.Google Scholar
McNaught, M. L. (1951). The utilization of non protein nitrogen in the bovine rumen. 7. A qualitative and quantitative study of the breakdown of carbohydrate which accompanies protein formation in bovine rumen contents during in vitro incubation. Biochem. J. 49, 325–32.Google Scholar
Oltjen, R. R. & Putman, P. A. (1966). Plasma amino acids and nitrogen retention by steers fed purified diets containing urea or isolated soy protein. J. Nutr. 89, 385–91.Google Scholar
Pearson, R. M. & Smith, J. A. B. (1943a). The utilization of urea in the bovine rumen. I. Methods of analysis of the rumen ingesta and preliminary experiments in vivo. Biochem. J. 37, 142–8.Google Scholar
Pearson, R. M. & Smith, J. A. B. (1943b). The utilization of urea in the bovine rumen. 3. The synthesis and breakdown of protein in rumen ingesta. Biochem. J. 37, 153–64.CrossRefGoogle ScholarPubMed
Phillipson, A. T., Dobson, M. J., Blackburn, T. H. & Brown, M. (1962). The assimilation of ammonia nitrogen by bacteria in the rumen of sheep. Br. J. Nutr. 16, 151–66.CrossRefGoogle ScholarPubMed
Schaadt, H., Johnson, R. R. & McClure, K. E. (1966). Adaptation to and palatability of urea, biuret and diammonium phosphate as non protein nitrogen sources for ruminants. J. anim. Sci. 25, 73–8.Google Scholar
Schwartz, H. M., Schoeman, C. A. & Farber, M. S. (1964). Utilization of urea by sheep. I. Rates of breakdown of urea and carbohydrate in vivo and in vitro. J. agric. Sci., Camb. 63, 289–96.Google Scholar
Smith, G. S., Dunbar, R. S., McLaren, G. A., Anderson, G. C. & Welch, J. A. (1960). Measurement of the adaption response to urea nitrogen utilization in the ruminant. J. Nutr. 71, 20–6.Google Scholar
Winter, K. A., Johnson, R. R. & Dehority, B. A. (1964). Metabolism of urea nitrogen by mixed cultures of rumen bacteria grown on cellulose. J. Dairy Sci. 47, 793–7.Google Scholar