Mexican sunflower [Tithonia diversifolia (Hemsl.) A. Gray] is an invasive plant, native to the New World, and an exemplary conflict species. It has been planted widely for its ornamental and soil fertility enhancement qualities and has become a notorious environmental weed in introduced habitats. Here we use a bioclimatic niche model (CLIMEX) to estimate the potential global distribution of this invasive plant under historical climatic conditions. We apply a future climate scenario to the model to assess the sensitivity of the modeled potential geographic range to expected climate changes to 2050. Under current climatic conditions, there is potential for substantial range expansion into southern Europe with moderate climate suitability, and in southern China with highly suitable climates. Under the near-term future climate scenario, there is potential for poleward range expansion in the order of 200 to 500 km. In the tropics, climatic conditions are likely to become less favorable due to the increasing frequency of supra-optimal temperatures. In areas experiencing Mediterranean or warm temperate climates, the suitability for T. diversifolia appears set to increase as temperatures warm. There are vast areas in North America, Europe, and Asia (particularly China and India) that can support ephemeral populations of T. diversifolia. One means of enjoying the aesthetic benefits of T. diversifolia in gardens while avoiding the unwanted environmental impacts where it invades is to prevent its spread into areas climatically suitable for establishment and only allow it to be propagated in areas where it cannot persist naturally.

The name of the genus Lycium originates from the Greek name lykion for a thorny shrub, derived from Lycia, the name of an ancient country in Asia Minor where a similar spiny shrub was found. The specific epithet ferocissimum comes from the Latin ferox, meaning “bold” or “fearless,” referring to the very spiny nature of the shrub (Green 1994; Parsons and Cuthbertson 2001).

The composition of nonnative floras is influenced by a region’s socioeconomic history, yet rarely are these factors studied alongside plant naturalization rates over time. Such information is especially critical for archipelagos, which often host large numbers of nonnative plants and would benefit from prevention of inter-island spread. We compiled the first record of occurrence and first record of naturalization for all naturalized plants in Hawai‘i alongside data on their origin, native climate types, taxonomy, and likely introduction pathway and compared rates of naturalization with socioeconomic trends. We found that the rate of total plant naturalizations has increased at a roughly constant rate during the past century without any sign of plateauing. However, this relatively steady increase is underlain by notable fluctuations in naturalization rates for different introduction pathways, with ornamentals increasing recently, while agriculture-related plants have decreased. Furthermore, this trend mirrors a shift from an agriculture-dominated economy to a tourism-based one associated with increases in both resident and tourist populations as well as general economic well-being. We further found that the average naturalized species spreads at a rate of 1.86 islands per decade, eventually occupying most major islands in the archipelago, and the rate of spread appears to be increasing since Hawai‘i’s economic shift. Our findings also emphasize the diversity of Hawai‘i’s nonnative flora, which originates from a variety of climates, continents, and taxonomic groups. We demonstrated that many nonnative species have native ranges that include temperate climates, which is important, because these climates typically co-occur with higher-elevation, remnant patches of native-dominated ecosystems in Hawai‘i. This study reveals trends that may help predict a species’ ability to naturalize and spread within and between islands, and we discuss management implications that may be extended to other regions.

The potential of Melaleuca quinquenervia (Cav.) S.T. Blake to reinvade cleared areas was evaluated over a 13-yr period that included two wildfires and the introduction of biological control agents. The first wildfire occurred in 1998 and was followed by a mean of 591.5 recruited seedlings m−2. Recruits from that fire were cleared 7 yr later in July 2005 for a second experiment to evaluate seedling recruitment into cleared areas. Seed rain, seedling recruitment and mortality, and sapling growth rates were measured in four plots located around individual large reproductive trees. A second natural wildfire in 2007 burned through those plots, leading to increases in seed rain followed by a pulse in recruitment of 21.04 seedlings m−2, 96.5% fewer than after the 1998 fire. Recruits in half of the plots around each tree were then treated with regular applications of an insecticide to restrict herbivory by biological control agents, while herbivory was not restricted in the other half. There was no difference in seedling mortality between treatments 1,083 d post-fire (2007) with 96.6% seedling mortality in the unrestricted herbivory treatment and 89.4% mortality in the restricted herbivory treatment. Recruits subjected to the restricted herbivory treatment grew taller than those in the unrestricted herbivory treatment, 101.3 cm versus 37.4 cm. Many of the recruits were attacked by the biological control agents, which slowed their growth. Although solitary M. quinquenervia trees retain some capacity to reinvade areas under specific circumstances, there was a downward trend in their overall invasiveness at this site, with progressively smaller recruitment cohorts due to biological control agents. Land managers should prioritize removing large reproductive trees over treating recently recruited populations, which can be left for many years for the biological control agents to suppress before any additional treatment would be needed.

Land managers require tools that improve understanding of suitable habitat for invasive plants and that can be incorporated into survey efforts to improve efficiency. Habitat suitability models (HSMs) contain attributes that can meet these requirements, but it is not known how well they perform, as they are rarely field-tested for accuracy. We developed ensemble HSMs in the state of Wisconsin for 15 species using five algorithms (boosted regression trees, generalized linear models, multivariate regression splines, MaxEnt, and random forests), evaluated performance, determined variables that drive suitability, and tested accuracy. All models had good model performance during the development phase (Area Under the Curve [AUC] > 0.7 and True Skills Statistic [TSS] > 0.4). While variable importance and directionality was species specific, the most important predictor variables across all of the species’ models were mean winter minimum temperatures, total summer precipitation, and tree canopy cover. Post model development, we obtained 5,005 new occurrence records from community science observations for all 15 focal species to test the models’ abilities to accurately predict results. Using a correct classification rate of 80%, just 8 of the 15 species correctly predicted suitable habitat (α ≤ 0.05). Exploratory analyses found the number of reporters of these new data and the total number of new occurrences reported per species contributed to increasing correct classification. Results suggest that while some models perform well on evaluation metrics, relying on these metrics alone is not sufficient and can lead to errors when utilized for surveying. We recommend any model should be tested for accuracy in the field before use to avoid this potential issue.

Arbuscular mycorrhizal fungi (AMF, Glomeromycota) are globally distributed symbionts of plant roots. Relationships with arbuscular mycorrhizae can provide crucial support for the establishment of any plant in an unfavorable environment. We hypothesized that invasions of neophytes are associated with changes in the colonization of native plants and early invaders (archeophytes) by AMF. We examined changes in AMF colonization in yarrow (Achillea millefolium L.) and wild carrot (Daucus carota L.) (native plants) and tansy (Tanacetum vulgare L.) and false oatgrass [Arrhenatherum elatius (L.) P. Beauv. ex J. Presl & C. Presl] (archeophytes) in response to the invasion of four neophytes from the Asteraceae family, namely great globethistle (Echinops sphaerocephalus L.), New York aster [Symphyotrichum novi-belgii (L.) G. L. Nesom agg.], annual fleabane [Erigeron annuus (L.) Pers.], and Canada goldenrod (Solidago canadensis L.). We found that the AMF colonization of the Asteraceae neophytes was high in the studied monodominant invasions, and the AMF colonization of the neophytes was higher than or equal to that of the studied native plants and archeophytes. Changes in plant dominance did not serve as predictors of the extent of AMF colonization of the native plants and archeophytes despite the invaded plots being associated with strong changes in the availability of primary and secondary mineral nutrients. The absence of a response of AMF colonization of native and archeophyte plant species to the invasion of neophytes suggests that AMF are passengers, rather than drivers, in the course of Asteraceae invasions in central European environments.

Invasive plants are expanding their ranges due to climate change, creating new challenges for invasive species management. Early detection and rapid response could address some nascent invasions, but limited resources make it impossible to monitor for every range-shifting species. Here, we aimed to create a more focused watch list by evaluating the impacts of 87 plant species projected to shift into northern New England (the states of Maine, New Hampshire, and/or Vermont). We used the Environmental Impact Classification for Alien Taxa (EICAT) protocol to evaluate all ecological impacts reported in the scientific literature, scoring ecological impacts from 1 (minimal concern) to 4 (major) depending on the level of reported impact. For each species, we also recorded any reported impacts on socioeconomic systems (agriculture, human health, or economics) as “present.” We found 24 range-shifting species with impacts on ecological communities, of which 22 have reported impacts in ecosystems common to northern New England. Almost all of these species also had impacts on socioeconomic systems and were available for purchase at ornamental plant retailers or online. Thus, these species can be considered high risk to northern New England with climate change based on their large negative impacts and potential to arrive quickly with deliberate human introduction. Our study demonstrates the use of impact assessments for creating targeted priority lists for invasive species monitoring and management.

Ventenata [Ventenata dubia (Leers) Coss.] is one of several annual grass invaders of the western United States. Ventenata dubia is documented reducing the forage availability for livestock and wildlife as well as lowering biodiversity in the Great Basin. This species has recently spread to the Great Plains, where it could bring these impacts with it. We attempt to answer questions on whether or not conservation practices, in this case removal of V. dubia with herbicide, result in recovery of forage resources and biodiversity. We answer these questions by measuring biomass, cover, and nutrient content 1-yr posttreatment at 9 sites in Sheridan County, WY, conducted in two years. Perennial grasses have higher crude protein and total digestible nutrients than V. dubia, and removal of V. dubia resulted in a positive perennial grass response both years. Further, the differences in pattern of growth between perennial and annual species, with annual grasses quickly senescing early in the year, make perennial grasses a more dependable forage base with higher available nutrients. Interestingly, total biomass and nutrient mass did not change after V. dubia removal due to equal replacement with perennial grasses. Species richness and diversity were unaffected by removal of V. dubia. Our results suggest that managing invasive annual grasses, particularly V. dubia, in the Northern Great Plains can improve forage resources for livestock and wildlife while maintaining species diversity. Therefore, proactive monitoring and management efforts to prevent spread should be prioritized in this region.

Risk assessments of biological invasions rarely account for native species performance and community features, but the assessment presented here could provide additional insights for management aimed at decreasing vulnerability or increasing resistance of a plant community to invasions. To gather information on the drivers of native plant communities’ vulnerability and resistance to invasion, we conducted a literature search and meta-analysis. Using the data we collected, we compared native and invasive plant performance between sites with high and low levels of invasion. We then investigated conditions under which native performance increased, decreased, or did not change with respect to invasive plants. We analyzed data from 214 publications summing to 506 observations. There were six main drivers of vulnerability to invasion: disturbance, decrease in resources, increase in resources, lack of biotic resistance, lack of natural enemies, and differences in propagule availability between native and invasive species. The two mechanisms of vulnerability to invasion associated with a strong decline in native plant performance were propagule availability and lack of biotic resistance. Native plants marginally benefited from enemy release and from decreases in resources, while invasive plants strongly benefited from both increased resources and lack of enemies. Fluctuation of resources, decreases and increases, were strongly associated with higher invasive performance, while native plants varied in their responses. These differences were particularly strong in instances of decreasing water or nutrients and of increasing light and nutrients. We found overall neutral to positive responses of native plant communities to disturbance, but natives were outperformed by invasive species when disturbance was caused by human activities. We identified ecosystem features associated with both vulnerability and resistance to invasion, then used our results to inform management aimed at protecting the native community.

Pale swallowwort [Vincetoxicum rossicum (Kleopow) Barbar.; syn.: Cynanchum rossicum (Kleopow) Borhidi] and black swallowwort [Vincetoxicum nigrum (L.) Moench; syn.: Cynanchum louiseae Kartesz & Gandhi] are invasive perennial viny milkweeds that have become prevalent across natural and managed habitats in northeastern North America. Southern blight of V. rossicum caused by the fungus Athelia rolfsii (Curzi) C. C. Tu & Kimbr. was reported at a New York county park in 2008, resulting in a decline in V. rossicum stands. The disease outbreak and persistence of the pathogen highlighted the potential of A. rolfsii for Vincetoxicum spp. control. To better characterize A. rolfsii’s pathogenicity and biology, we studied virulence to adult Vincetoxicum spp., spatiotemporal attributes of the Southern blight epidemic at the discovery site over 4 yr, and sclerotial survival over 2 yr. Disease incidence and severity were high for both Vincetoxicum spp. in misting chamber experiments. The spatiotemporal spread patterns of Southern blight in V. rossicum suggest the epidemic in the first year of monitoring (2016) was already highly aggregated and that subsequent spread was limited and resulted in significant local aggregation. Sclerotial survival studies at two locations (Pittsford and Ithaca, NY) demonstrated the A. rolfsii isolates can overwinter in upstate New York and are pathogenic to Vincetoxicum spp. the subsequent season. However, shallow burial of sclerotia more rapidly reduced survival compared with placement on the soil surface. Overwinter survival of A. rolfsii sclerotia in New York is notable, as this pathogen is typically associated with subtropical and tropical regions. Broadcast applications of the pathogen would be needed for widespread Vincetoxicum control at a site, but even restricting releases to select locations would not prevent pathogen movement off-site via water or machinery. The known risks of the A. rolfsii isolate to other broadleaf plants in natural and agricultural settings suggest a low feasibility of use for the biological control of Vincetoxicum spp.

Invasive species negatively impact vegetation communities. Invasive European common reed [Phragmites australis (Cav.) Trin. ex Steud. ssp. australis] is rapidly spreading throughout North American wetlands. As such, the suppression of P. australis populations is a goal of many managers, as its removal should provide an opportunity to restore native vegetation communities. In Ontario, managers applied a glyphosate-based herbicide to more than 400 ha of P. australis in ecologically significant coastal marshes, representing the first time this tool was used over standing water to suppress an invasive species in Canada. Using a before–after–control–impact monitoring design, we evaluated the efficacy of glyphosate-based herbicide at removing P. australis along a water-depth gradient and assessed the recovery of the vegetation community for 2 yr after treatment in relation to reference conditions. We found that herbicide suppressed more than 99% of P. australis 1 yr after treatment and worked effectively along the entire water-depth gradient (10 to 48 cm). However, the post-treatment vegetation community remains distinctive from reference marsh 2 yr after treatment. In many plots where P. australis was removed, nonnative European frog-bit (Hydrocharis morsus-ranae L.) is now dominant, likely aided by high lake-water levels.

Eradication of alien invasive species in the soil with steam as an alternative to chemical fumigation may allow contaminated soil to be reused. We have investigated steam disinfestation of soil to combat invasive plant species in three experiments including different temperatures and exposure durations using a prototype stationary soil-steaming device. The experiments included effects on seed germination of bigleaf lupine (Lupinus polyphyllus Lindl.), ornamental jewelweed (Impatiens glandulifera Royle), and wild oat (Avena fatua L.; one population from Poland and one from Norway), as well as effects on sprouting rhizome fragments of Canada goldenrod (Solidago canadensis L.) and Bohemian knotweed (Reynoutria x bohemica Chrtek & Chrtková). In Experiment 1, we tested four different soil temperatures of 64, 75, 79, and 98 C with an exposure duration of 90 s. In Experiments 2 and 3, we tested exposure durations of 30, 90, and 180 s and 90, 180, and 540 s, respectively, at 98 C. Seed pretreatment of 14 d cooling for L. polyphyllus and I. glandulifera, no seed pretreatment and 12-h moistening for A. fatua populations, and 5- and 10-cm cutting size for R. x bohemica were applied. Our results showed germination/sprouting was inhibited at 75 C for I. glandulifera (for 90 s) and 98 C for the other species; however, longer exposure duration was needed for L. polyphyllus. While 30 s at 98 C was enough to kill A. fatua seeds and S. canadensis and R. x bohemica rhizome fragments, 180-s exposure duration was needed to kill L. polyphyllus seeds. The results showed promising control levels of invasive plant propagules in contaminated soil by steaming, supporting the steam treatment method as a potential way of disinfecting soil to prevent dispersal of invasive species.

Strong competitive ability of introduced plant species has been frequently stated as a key factor promoting successful invasion. The dynamics of invasive species may depend on their abilities to compete for resources and exploit disturbances relative to native species. This study compares the growth performance of the invasive blackberry (Rubus niveus Thunb.) with four of the most common woody native species of the Scalesia forest in the Galapagos Islands. Using a series of greenhouse and field studies, the growth rate and biomass production of native species alone and in competition with R. niveus was compared under different water and light stress conditions. Rubus niveus showed a faster growth rate and biomass production than the native species as well as a broad tolerance to light and water stress conditions. Competitive ability was also assessed by looking at the seedbank and regeneration processes after herbicide control in the field. Although the number of R. niveus seedlings that germinated from the soil samples was considerably larger than that of native species, recruitment of the invasive on the experimental plots was limited. Overall, R. niveus showed superior competitive ability over native species with comparable growth forms, suggesting a possible mechanism that enables R. niveus to successfully invade a wide range of habitats in the Galapagos Islands. Implementing an integrated management strategy including biological control, seedbank reduction, and active revegetation, should improve the efficiency of R. niveus management, enabling the restoration of degraded vegetation in the Galapagos.

Yellow-flag iris (Iris pseudacorus L.) is a nonnative, invasive wetland plant that disrupts riparian ecosystem processes and is widely distributed across the United States and Canada. Due to its physiological and morphological characteristics, I. pseudacorus has the capacity to exclude native vegetation and form extensive monocultures in both lotic and lentic wetland systems. Methods commonly used to manage I. pseudacorus include manual (e.g., hand pulling, digging) and mechanical (e.g., mowing) treatments for small populations and herbicide applications for larger populations; however, herbicide applications near water may be prohibited due to label restrictions. The objective of this research was to evaluate cattle trampling as a nonchemical method to reduce I. pseudacorus in riparian habitats. A greenhouse study was conducted to investigate the effects of inundation and two different timings of simulated trampling on I. pseudacorus density, height, and soluble sugar concentrations in the rhizomes. A complementary field demonstration was established on a ranch in northwestern Nebraska to evaluate cattle trampling effects on I. pseudacorus density and height after two consecutive years. Simulated cattle trampling in the greenhouse had no effect on I. pseudacorus density or height of non-inundated samples. However, combining trampling with inundation reduced I. pseudacorus density from a median of 10 I. pseudacorus per pot to 0 I. pseudacorus per pot and median height from 0.35 m to 0 m by the conclusion of the study. Additionally, the field demonstration resulted in reductions of both density and height of I. pseudacorus after two consecutive years (72% and 67% reduction, respectively). Soluble sugar concentrations were not impacted by any treatment.

Many restoration projects rely on invasive plant removal to restore ecosystems. However, success of restoration efforts relying on invasive removal can be jeopardized, because in addition to displacing native plants, invasives can also dramatically impact soils. Many studies have documented invasives’ effects on soil chemistry and microbiota. While European beachgrass [Ammophila arenaria (L.) Link] is a worldwide invasive problem in coastal dunes outside northern Europe, little attention has been paid to effects of this species on soil chemistry following invasion, even though it establishes persistent, dense monocultures. In our study, we evaluated effects of A. arenaria invasion on soil chemistry of coastal dunes at Point Reyes National Seashore (PRNS); persistence of effects following removal by mechanical or herbicide treatment (legacy effects); and effects of treatment independent of invasion. Dune restoration efforts at PRNS have met with mixed success, especially in herbicide-treated backdunes, where decomposition of dead A. arenaria has been greatly delayed. Based on results, invasion impacted 74% of 19 variables assessed, although there was a significant interaction in many cases with successional status (earlier vs. later). Almost 60% of invasion effects persisted after restoration, with legacy effects prevalent in herbicide-treated backdunes where sand deposition from adjacent beaches could not mitigate effects as it could in herbicide-treated foredunes. Mechanical removal—or inversion of invaded surface soils with less-contaminated subsoils—resulted in fewer legacy effects, but more treatment effects, primarily in backdunes. Soil chemistry may decelerate decomposition of A. arenaria due to the limited nitrogen (N) available to enable microbial breakdown of the high carbon(C):N (70.8:1) material, but microbial factors probably play a more important role. Success of restoration at PRNS may not be fully realized until legacy effects are resolved through additional actions such as inoculation with healthy microbiomes or necromass reduction through controlled burning.

Using species distribution models (SDMs), we predicted the distribution of 170 plant species under different climatic scenarios (current and future climatic conditions) and used this information to create invasion risk maps to identify potential invasion hot spots in California. The risk of invasion by individual species was also assessed using species’ predicted area in combination with some biological traits associated with invasiveness (growth form, reproduction mechanisms, and age of maturity). A higher number of species would find suitable climatic conditions along the coast; the Central Western (CW) and South Western (SW) were ecoregions where a higher number of species were predicted. Overall, hot spots of species distribution were similar under current and future climatic conditions; however, individual species’ predicted area (increase or decrease) was variable depending on the climate change scenario and the greenhouse gas emission. Out of the 170 species assessed, 22% ranked as high-risk species, with herbs, grasses, and vines accounting for 78% within this risk class, and a high proportion (67%) of Asteraceae species ranked as high risk. This study suggests that current climatic conditions of the central and south coastal regions of California would be considered as hot spots of new invasions, and for some species this risk might increase with hotter and drier future climatic conditions.

Mesocosm studies were conducted in 2020 to evaluate the effects of carrier volume and application method on waterhyacinth [Eichhornia crassipes (Mart.) Solms] response to 2,4-D, glyphosate, and diquat. Carrier volumes of 935, 467, and 187 L ha−1 were applied using either a conventional stream, conventional cone, adjustable cone, or a drizzle-stream spray pattern. Reducing carrier volume from 935 L ha−1 reduced spray coverage up to 60%, depending on application method. However, reducing carrier volume did not diminish efficacy of any herbicide or application method. Alternatively, E. crassipes control from 2,4-D increased 10% to 26% when applied using 187 L ha−1 compared with 935 L ha−1. Likewise, E. crassipes biomass was reduced 91% when 2,4-D was applied using 935 L ha−1; however, treatment applied at 187 L ha−1 resulted in 99% biomass reduction. In general, 2,4-D resulted in roughly 10% greater control when conventional or adjustable cone applications were used compared with either stream applications. Eichhornia crassipes control at 7 d after treatment (DAT) from diquat increased with decreasing carrier volumes; however, treatment effects in diquat experiments were not detected at other evaluation intervals. Glyphosate efficacy was highly influenced by carrier volume, as E. crassipes control increased up to 61% when applied using 187 L ha−1 compared with 935 L ha−1. Moreover, E. crassipes biomass reduction increased from 55% in the 935 L ha−1 treatment to 97% in the 187 L ha−1 treatments. Glyphosate application methods consisting of conventional stream or conventional cone sprayers resulted in slightly increased E. crassipes control by 28 DAT; however, no differences among application methods were observed in E. crassipes biomass data. These data support further evaluations of alternative application techniques for E. crassipes control under field conditions and for other herbicides and aquatic plant species.

Scientists and natural resource managers require suitable vegetation survey methods to assess the success of rangeland restoration projects. Visual estimation and point intercept methods are commonly used to evaluate vegetation cover. This study compared the performance of one visual (quadrat-based) and two line-point intercept (LPI, canopy and basal) methods to assess biodiversity and cover and to estimate biomass production on sites invaded by introduced annual grasses across Wyoming, USA. Greater species richness and higher Shannon index values were measured in quadrats, while introduced annual and native perennial graminoid cover values were higher in LPI canopy in general. Overall, these outcomes indicate quadrats as the most suitable survey method when biodiversity monitoring is the primary objective, while suggesting LPI canopy when monitoring vegetation cover is prioritized. Finally, our regression models indicated quadrat-based estimates as the most reliable to predict introduced annual and native perennial graminoid biomass.

Knotweed species in the genus Reynoutria are native to eastern Asia but have become noxious weeds in Europe and North America. In the United States, invasive populations of Japanese knotweed (Reynoutria japonica Houtt.), giant knotweed [Reynoutria sachalinensis (F. Schmidt) Nakai], and their interspecific hybrid known as Bohemian knotweed (R. × bohemica Chrtek & Chrtková) continue to expand their ranges. Although these plants are among the most invasive terrestrial species, there are relatively few molecular tools for identifying the parental species, the F1 hybrid, or subsequent hybrids or introgressed individuals. We studied Reynoutria populations in Wisconsin, a state where all three taxa grow, to determine whether molecular data would be useful for distinguishing species and identifying hybrids. We obtained DNA sequence data from the plastid matK gene and the nuclear LEAFY gene and compared these to previously published sequences. Data from the uniparentally inherited matK region included haplotypes attributable to R. japonica and R. sachalinensis. Nuclear data indicated that R. sachalinensis plants are most similar to native plants in Japan, with two Wisconsin accessions exhibiting a monomorphic genotype for the LEAFY gene. Three Wisconsin accessions of R. japonica were each characterized by having three distinct kinds of LEAFY sequence. Most plants in our study were found to possess two or three phylogenetically distinct copies of the LEAFY gene, with the copies being most closely related to R. japonica and R. sachalinensis, respectively, and these were inferred to be interspecific hybrids. Altogether, five kinds of interspecific hybrids were identified, reflecting various combinations of LEAFY sequence types from the parental species. The widespread existence of hybrid plants in Wisconsin, many of which are morphologically identifiable as R. japonica, indicates a cryptic genetic diversity that should be examined more broadly in North America using molecular tools.