Hostname: page-component-78c5997874-v9fdk Total loading time: 0 Render date: 2024-11-19T14:45:17.241Z Has data issue: false hasContentIssue false
  • English
  • Français

Plastid DNA Analysis Reveals Cryptic Hybridization in Invasive Dalmatian Toadflax (Linaria dalmatica) Populations

Published online by Cambridge University Press:  20 January 2017

Andrew Boswell ,
Sharlene E. Sing  and
Sarah M. Ward
Andrew Boswell
Affiliation:
Department of Soil and Crop Sciences, Colorado State University, Fort Collins, CO 80523
Sharlene E. Sing
Affiliation:
Rocky Mountain Research Station, U.S. Department of Agriculture-U.S. Forest Service, Bozeman, MT 59717
Sarah M. Ward*
Affiliation:
Department of Soil and Crop Sciences, Colorado State University, Fort Collins, CO 80523
*
Corresponding author's E-mail: [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Gene flow between Dalmatian toadflax (DT) and yellow toadflax (YT), both aggressive invaders throughout the Intermountain West, is creating hybrid populations potentially more invasive than either parent species. To determine the direction of gene flow in these hybrid populations, species-diagnostic cytoplasmic markers were developed. Markers were based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) polymorphisms in the trnT-D chloroplast DNA (cpDNA) region digested with Alu1, and single-nucleotide polymorphisms (SNPs) in the matK and trnL-F chloroplast-barcoding regions. Four hybrid toadflax populations sampled from Colorado, Montana, and Washington contained both DT and YT cytoplasm, with YT predominating; 25 individuals from a fifth hybrid population from Idaho all had identical YT cpDNA haplotypes. Thirteen plants from two Colorado populations, assumed to be DT based on morphology and geographic isolation from any known YT population, were found to have YT cpDNA haplotypes. These results indicate that gene flow between invasive YT and DT populations is more widespread that previously realized and confirms that cryptic introgression of YT alleles has occurred in multiple western U.S. DT populations. The presence of YT genetic material in presumed DT populations may negatively affect host recognition and establishment by biocontrol agents used for toadflax management.

Keywords

Dalmatian toadflax, Linaria dalmatica (L.) P. Mill.yellow toadflax, Linaria vulgaris P. Mill.cpDNADNA barcodingcryptic hybridizationgene flowhaplotype
Type
Research
Information
Invasive Plant Science and Management , Volume 9 , Issue 2 , June 2016 , pp. 112 - 120
Copyright
Copyright © Weed Science Society of America 

References

Literature Cited

Alex, J (1962) The taxonomy, history, and distribution of Linaria dalmatica . Can J Bot 40: 295–307 Google Scholar
Arnold, RM (1982) Pollination, predation and seed set in Linaria vulgaris (Scrophulariaceae). Am Midl Nat 107: 360–369 Google Scholar
Birky, CW (2001) The inheritance of genes in mitochondria and chloroplasts: laws, mechanisms, and models. Ann Rev Genet 35: 125–148 Google Scholar
Brown, LS (2008) Genetic Variation of the Invasive Linaria dalmatica in Its Introduced Range in Western North America and the Impact of Its Predominant Biological Control Agent, Mecinus janthinus. MS thesis. Moscow, ID: University of Idaho. 91 pGoogle Scholar
Chater, AD, Valdés, B, Webb, DA (1972) Linaria Miller. Pages 226–236 in Tutin, VG, Heywood, VH, eds. Flora Europaea. Volume 3. Cambridge, U.K.: Cambridge University Press Google Scholar
Corriveau, JL, Coleman, AW (1988) Rapid screening method to detect potential biparental inheritance of plastid DNA and results for over 200 angiosperms. Am J Bot 75: 1443–1458 Google Scholar
Davis, PH (1978) Flora of Turkey and the East Aegean Islands. Edinburgh, U.K.: Edinburgh University Press 826 pGoogle Scholar
De Clerck-Floate, R, Richards, KW (1997) Pollination ecology and biocontrol: developing release strategies for seed-feeding insects on Dalmatian toadflax. Acta Hortic 437: 379–384 Google Scholar
Fernández-Mazuecos, M, Blanco-Pastor, JL, Vargas, P (2013) A phylogeny of toadflaxes (Linaria Mill.) based on nuclear internal transcribed spacer sequences: systematic and evolutionary consequences. Int J Plant Sci 174: 234–249 Google Scholar
Field, DL, Ayre, DJ, Whelan, RJ, Young, AG (2011) Patterns of hybridization and asymmetrical gene flow in hybrid zones of the rare Eucalyptus aggregata and common E. rubida . Heredity 106: 841–853 Google Scholar
Fisher, RA, Yates, F (1963) Statistical Tables: For Biological, Agricultural and Medical Research. 6th edn. Edinburgh, U.K.: Oliver and Boyd. 148 pGoogle Scholar
Hartl, D (1974) Scrophulariaceae. Pages 78–81 in Hegi, G, ed. Illustrierte Flora von Mitteleuropa. Munich: Carl Hanser Verlag Google Scholar
Hollingsworth, PM, Graham, SW,and Little, DP (2011) Choosing and using a plant DNA barcode. PLoS One 6: e19254. DOI: 10.1371/journal.pone.0019254Google Scholar
Kress, WJ, Erickson, DL (2007) A two-locus global DNA barcode for land plants: the coding rbcL gene complements the non-coding trnH/psbA spacer region. PLoS One 2: e508. DOI: 10.1371/journal.pone.0000508Google Scholar
Lahaye, R, van der Bank, M, Bogarin, D, Warner, J, Pupulin, F, Gigot, G, Maurin, O, Duthoit, S, Barraclough, TG, Savolainen, V (2008) DNA barcoding the floras of biodiversity hotspots. Proc Natl Acad Sci USA 105: 2923–2928 Google Scholar
Lewis, D, Crowe, LK (1958) Unilateral interspecific incompatibility in flowering plants. Heredity 12: 233–256 Google Scholar
Liu, J, Moller, M, Gao, LM, Zhang, DQ, Li, DZ (2011) DNA barcoding for the discrimination of Eurasian yews (Taxus L., Taxaceae) and the discovery of cryptic species. Mol Ecol Resour 11: 89–100 Google Scholar
Mack, RN (2003) Plant naturalizations and invasions in the eastern United States: 1634–1860. Ann Mo Bot Gard 90: 77–90 Google Scholar
Muranishsi, S, Tamaki, I, Setsuko, S, Tomaru, N (2013) Asymmetric introgression between Magnolia stellata and M. salicifolia at a site where the two species grow sympatrically. Tree Genet Genomes 9: 1005–1015 Google Scholar
Niketić, M, Tomović, G (2008) Taxonomy and nomenclature of the Linaria genistifolia complex (Plantaginaceae-Antirrhineae) in S.E. Europe and Anatolia. Taxon 57: 619–629 Google Scholar
Palmer, JD, Hebron, LA (1988) Plant mitochondrial DNA evolves rapidly in structure, but slowly in sequence. J Mol Evol 28: 87–97 Google Scholar
Pauchard, A, Alaback, PB, Edlund, EG (2003) Plant invasions in protected areas at multiple scales: Linaria vulgaris (Scrophulariaceae) in the West Yellowstone area. West N Am Naturalist 63: 416–428 Google Scholar
Rahme, J, Widmer, A, Karrenberg, S (2009) Pollen competition as an asymmetric reproductive barrier between two closely related Silene species. J Evol Biol 22: 1937–1943 Google Scholar
Ravi, V, Khurana, JP, Tyagi, AK, Khurana, P (2008) An update on chloroplast genomes. Plant Syst Evol 271: 101–122 Google Scholar
Saner, MA, Clements, DR, Hall, MR, Doohan, DJ, Crompton, CW (1995) The biology of Canadian weeds. 105. Linaria vulgaris Mill. Can J Plant Sci 75: 525–537 Google Scholar
Sang, T, Crawford, DJ, Stuessy, TF (1997) Chloroplast DNA phylogeny, reticulate evolution and biogeography of Paeonia (Paeoniaceae). Am J Bot 84: 1120–1136 Google Scholar
Shaw, J, Lickey, EB, Beck, JT, Farmer, SB, Liu, WS, Miller, J, Small, RL (2005) The tortoise and the hare II: relative utility of 21 noncoding plastid DNA sequences for phylogenetic analysis. Am J Bot 92: 142–166 Google Scholar
Starr, TN, Gadek, KE, Yoder, JB, Flatz, R, Smith, CI (2013) Asymmetric hybridization and gene flow between Joshua trees (Agavaceae: Yucca) reflect differences in pollinator host specificity. Mol Ecol 22: 437–449 Google Scholar
Sutton, DA (1988) A Revision of the Tribe Antirrhineae. London: Oxford University Press. 584 pGoogle Scholar
Sutton, J, Stohlgren, T, Beck, KG (2007) Predicting yellow toadflax infestations in the Flat Tops Wilderness of Colorado. Biol Invasions 9: 783–793 Google Scholar
Taberlet, P, Gielly, L, Pautou, G, Bouvet, G (1991) Universal primers for amplification of three non-coding regions of plastid DNA. Plant Mol Biol 17: 1105–1109 Google Scholar
Tiffin, P, Olson, MS, Moyle, LC (2001) Asymmetrical crossing barriers in angiosperms. Proc R Soc Lond B Biol Sci 268: 861–867 Google Scholar
Toševski, I, Jović, J, Krstić, O, Gassmann, A (2013) PCR-RFLP-based method for reliable discrimination of cryptic species within Mecinus janthinus species complex (Mecinini, Curculionidae) introduced in North America for biological control of invasive toadflaxes. Biocontrol 58: 563–573 Google Scholar
Toševski, I, Caldara, R, Jović, J, Hernández-Vera, G, Baviera, C, Gassmann, A, Emerson, BC (2011) Morphological, molecular and biological evidence reveal two cryptic species in Mecinus janthinus Germar (Coleoptera, Curculionidae), a successful biological control agent of Dalmatian toadflax, Linaria dalmatica (Lamiales, Plantaginaceae). Syst Entomol 36: 741–753 Google Scholar
Turner, MF (2012) Viability and Invasive Potential of Hybrids between Yellow Toadflax (Linaria vulgaris) and Dalmatian Toadflax (Linaria dalmatica). Ph.D. dissertation. Fort Collins, CO: Colorado State University. 130 pGoogle Scholar
[USDA-NRCS] U.S. Department of Agriculture–Natural Resources Conservation Service (2016) Plants Database. http://plants.usda.gov. Accessed April 1, 2016.Google Scholar
Vujnovic, K, Wein, RW (1997) The biology of Canadian weeds. 106. Linaria dalmatica (L.) Mill. Can J Plant Sci 77: 483–491 Google Scholar
Ward, SM, Reid, SD, Harrington, J, Sutton, J, Beck, KG (2008) Genetic variation in invasive populations of yellow toadflax (Linaria vulgaris) in the western United States. Weed Sci 56: 394–399 Google Scholar
Ward, SM, Sing, SE, Turner, MF, Fleischmann, CE (2009) Hybridization between invasive populations of Dalmatian toadflax (Linaria dalmatica) and yellow toadflax (Linaria vulgaris). Invasive Plant Sci Manag 2: 369–378 Google Scholar
Whitney, KD, Randell, RA, Riesebery, LH (2006) Adaptive introgression of herbivore resistance traits in the weedy sunflower Helianthus annuus . Am Nat 167: 794–807 Google Scholar
Wilson, L, Sing, SE, Piper, GL, Hansen, RW, De Clerck-Floate, R, MacKinnon, DK, Randall, CB (2005) Biology and Biological Control of Dalmatian and Yellow Toadflax. Morgantown, WV: USDA Forest Service Google Scholar