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Accepted manuscript

Nuclear DNA markers to screen for hybridization between Phragmites australis americanus and other Phragmites subspecies

Published online by Cambridge University Press:  03 March 2025

Douglas L. Wendell Open the ORCID record for Douglas L. Wendell [Opens in a new window] ,
Telissa Wilson Open the ORCID record for Telissa Wilson [Opens in a new window] ,
V. Novellus Washington  and
Isabella J. Limbert
Douglas L. Wendell*
Affiliation:
Associate Professor, Department of Biological Sciences, Oakland University, Rochester, MI, USA
Telissa Wilson
Affiliation:
Natural Resource Scientist, Washington State Department of Agriculture, Plant Pathology and Molecular Diagnostics Laboratory, Tumwater, WA, USA
V. Novellus Washington
Affiliation:
Department of Biological Sciences, Oakland University, Rochester, MI, USA
Isabella J. Limbert
Affiliation:
Department of Biological Sciences, Oakland University, Rochester, MI, USA
*
Author for correspondence: Douglas L. Wendell; Email [email protected]
Rights & Permissions [Opens in a new window]

Abstract

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We report a pair of simple PCR-RFLP assays based on two independently segregating nuclear genes which can differentiate North American native Phragmites australis subsp. americanus Saltonst., P.M. Peterson & Soreng from other lineages. Because nuclear markers are inherited biparentally, researchers can also use them to screen for F1 hybrids between americanus and the other lineages. We show that a previously described assay based on an indel in the nuclear gene NRT2 consistently identifies a wide range of americanus haplotypes and distinguishes them from the Gulf Coast type Phragmites australis (Cav.) Trin. ex Steud. ssp. berlandieri (E. Fourn.) Saltonst. & Hauber. We also demonstrate a new PCR-RFLP assay for a previously described diagnostic single nucleotide polymorphism adjacent to the PaGT4 microsatellite marker which also distinguishes americanus from the other lineages. In addition, we report the first case of Asian haplotype AS identified in North America and make recommendations for its detection. Our findings expand the tools available to those monitoring for invasion by introduced Phragmites in North America.

Keywords

Phragmites hybridization genetic identification DNA markers PCR-RFLP
Type
Research Article
Information
Invasive Plant Science and Management , Accepted manuscript , pp. 1 - 15
Creative Commons
Creative Common License - CCCreative Common License - BYCreative Common License - NCCreative Common License - ND
This is an Open Access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is unaltered and is properly cited. The written permission of Cambridge University Press must be obtained for commercial re-use or in order to create a derivative work.
Copyright
© The Author(s), 2025. Published by Cambridge University Press on behalf of Weed Science Society of America