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Mislabeling of an Invasive Vine (Celastrus orbiculatus) as a Native Congener (C. scandens) in Horticulture

Published online by Cambridge University Press:  14 December 2017

David N. Zaya ,
Stacey A. Leicht-Young ,
Noel B. Pavlovic ,
Christopher S. Hetrea  and
Mary V. Ashley
David N. Zaya*
Affiliation:
Graduate Student, Undergraduate Student, and Professor, Department of Biological Sciences, University of Illinois at Chicago, 845 West Taylor Street, Chicago IL 60607
Stacey A. Leicht-Young
Affiliation:
Postdoctoral Fellow and Plant Ecologist, U.S. Geological Survey, Great Lakes Science Center, 1574 North 300 East, Chesterton, IN 46304
Noel B. Pavlovic
Affiliation:
Postdoctoral Fellow and Plant Ecologist, U.S. Geological Survey, Great Lakes Science Center, 1574 North 300 East, Chesterton, IN 46304
Christopher S. Hetrea
Affiliation:
Graduate Student, Undergraduate Student, and Professor, Department of Biological Sciences, University of Illinois at Chicago, 845 West Taylor Street, Chicago IL 60607
Mary V. Ashley
Affiliation:
Graduate Student, Undergraduate Student, and Professor, Department of Biological Sciences, University of Illinois at Chicago, 845 West Taylor Street, Chicago IL 60607
*
*Corresponding author’s E-mail: [email protected]
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Abstract

The horticultural industry is an important source of invasive ornamental plant species, which is part of the motivation for an increased emphasis on using native alternatives. We were interested in the possibility that plants marketed in the midwestern United States as the native Celastrus scandens, or American bittersweet, were actually the difficult-to-distinguish invasive Celastrus orbiculatus (oriental bittersweet) or hybrids of the two species. We used nuclear microsatellite DNA loci to compare the genetic identities of 34 plants from 11 vendors with reference plants from wild populations of known species identity. We found that 18 samples (53%) were mislabeled, and 7 of the 11 vendors sold mislabeled plants. Mislabeled plants were more likely to be purchased through Internet or phone order shipments and were significantly less expensive than accurately labeled plants. Vendors marketed mislabeled plants under five different cultivar names, as well as unnamed strains. Additionally, the most common native cultivar, ‘Autumn Revolution,’ displays reproductive characteristics that diverge from the typical C. scandens, which could be of some concern. The lower price and abundance of mislabeled invasive plants introduces incentives for consumers to unknowingly contribute to the spread of C. orbiculatus. Revealing the potential sources of C. orbiculatus is critical for controlling further spread of the invasive vine and limiting its impact on C. scandens populations.

Keywords

American bittersweet, Celastrus scandens L.oriental bittersweet, Celastrus orbiculatus ThunbHorticultural industryinvasive speciesmicrosatellite DNAmislabelingmolecular testing
Type
Research and Education
Information
Invasive Plant Science and Management , Volume 10 , Issue 4 , December 2017 , pp. 313 - 321
Copyright
© Weed Science Society of America, 2017 

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Footnotes

a

Current address of first author: Illinois Natural History Survey, 1816 South Oak Street, Champaign, IL 61820

b

current address of second author: Rummel, Klepper and Kahl, 700 East Pratt Street, Suite 500, Baltimore, MD 21202

Associate Editor for this paper: Marie Jasieniuk, University of California, Davis.

References

Literature Cited

Ashton, IW, Lerdau, MT (2008) Tolerance to herbivory, and not resistance, may explain differential success of invasive, naturalized, and native North American temperate vines. Divers Distrib 14:169–178 Google Scholar
Bailey, R, inventor; Bailey Nurseries, Inc., assignee (2009) Mar 10. Celastrus plant named ‘Bailumn.’ US patent USPP19,811Google Scholar
Burghardt, KT, Tallamy, DW, Shriver, WG (2009) Impact of native plants on bird and butterfly biodiversity in suburban landscapes. Conserv Biol 23:219–224 Google Scholar
Del Serrone, P, Attorri, L, Gallinella, B, Gallo, FR, Federici, E, Palazzino, G (2006) Molecular identification of Panax ginseng C.A. Meyer in ginseng commercial products. Nat Prod Commun 1:1137–1140 Google Scholar
Del Tredici, P (2014) Untangling the twisted tale of oriental bittersweet. Arnoldia 71:2–18 Google Scholar
Drew, J, Anderson, N, Andow, D (2010) Conundrums of a complex vector for invasive species control: a detailed examination of the horticultural industry. Biol Invasions 12:2837–2851 Google Scholar
Dreyer, GD, Baird, LM, Fickler, C (1987) Celastrus scandens and Celastrus orbiculatus—comparisons of reproductive potential between a native and introduced woody vine. B Torrey Bot Club 114:260–264 Google Scholar
EDDMapS. (2017). Early Detection & Distribution Mapping System. University of Georgia–Center for Invasive Species and Ecosystem Health. http://www.eddmaps.org. Accessed June 1, 2017Google Scholar
Falush, D, Stephens, M, Pritchard, JK (2003) Inference of population structure: extensions to linked loci and correlated allele frequencies. Genetics 164:1567–1587 CrossRefGoogle ScholarPubMed
Fan, LL, Zhu, S, Chen, HB, Yang, DH, Cai, SQ, Komatsu, K (2009) Identification of the botanical source of Stemonae Radix based on polymerase chain reaction with specific primers and polymerase chain reaction-restriction fragment length polymorphism. Biol Pharm Bull 32:1624–1627 CrossRefGoogle ScholarPubMed
Feng, T, Liu, S, He, XJ (2010) Molecular authentication of the traditional Chinese medicinal plant Angelica sinensis based on internal transcribed spacer of nrDNA. Electron J Biotechn 13. doi: 10.2225/vol13-issue1-fulltext-13 Google Scholar
Fike, J, Niering, WA (1999) Four decades of old field vegetation development and the role of Celastrus orbiculatus in the northeastern United States. J Veg Sci 10:483–492 Google Scholar
Honjo, M, Ueno, S, Tsumura, Y, Handa, T, Washitani, I, Ohsawa, R (2008) Tracing the origins of stocks of the endangered species Primula sieboldii using nuclear microsatellites and chloroplast DNA. Conserv Genet 9:1139–1147 Google Scholar
Hou, D (1955) A revision of the genus Celastrus . Ann Mo Bot Gard 42:215–302 Google Scholar
Johnson, LMK, Galloway, LF (2008) From horticultural plantings into wild populations: movement of pollen and genes in Lobelia cardinalis . Plant Ecol 197:55–67 CrossRefGoogle Scholar
Lehan, NE, Murphy, JR, Thorburn, LP, Bradley, BA (2013) Accidental introductions are an important source of invasive plants in the continental United States. Am J Bot 100:1287–1293 Google Scholar
Leicht, SA (2005). The Comparative Ecology of an Invasive Bittersweet Species (Celastrus orbiculatus) and Its Native Congener (C. scandens). Ph.D Dissertation. Storrs, CT: University of Connecticut. 162 pGoogle Scholar
Leicht-Young, SA, Latimer, AM, Silander, JA (2011) Lianas escape self-thinning: experimental evidence of positive density dependence in temperate lianas Celastrus orbiculatus and C. scandens . Perspect Plant Ecol 13:163–172 Google Scholar
Leicht-Young, SA, Pavlovic, NB (2015) Lianas as invasive species in North America. Pages 429–442 in Schnitzer SA, Bongers F, Burnham RJ, Putz FE, eds. Ecology of Lianas. New York, NY: Wiley Google Scholar
Leicht-Young, SA, Pavlovic, NB, Grundel, R, Frohnapple, KJ (2007a) Distinguishing native (Celastrus scandens L.) and invasive (C. orbiculatus Thunb.) bittersweet species using morphological characteristics. J Torrey Bot Soc 134:441–450 Google Scholar
Leicht-Young, SA, Silander, JA, Latimer, AM (2007b) Comparative performance of invasive and native Celastrus species across environmental gradients. Oecologia 154:273–282 Google Scholar
LeRoy, A, Potter, E, Woo, HH, Heber, D, Hirsch, AM (2002) Characterization and identification of alfalfa and red clover dietary supplements using a PCR-based method. J Agric Food Chem 50:5063–5069 Google Scholar
Lin, WY, Chen, LR, Lin, TY (2008) Rapid authentication of Bupleurum species using an array of immobilized sequence-specific oligonucleotide probes. Planta Med 74:464–469 Google Scholar
Mack, RN, Erneberg, M (2002) The United States naturalized flora: largely the product of deliberate introductions. Ann Mo Bot Gard 89:176–189 Google Scholar
Manel, S, Berthier, P, Luikart, G (2002) Detecting wildlife poaching: identifying the origin of individuals with Bayesian assignment tests and multilocus genotypes. Conserv Biol 16:650–659 Google Scholar
Manissorn, J, Sukrong, S, Ruangrungsi, N, Mizukami, H (2010) Molecular phylogenetic analysis of Phyllanthus species in Thailand and the application of polymerase chain reaction-restriction fragment length polymorphism for Phyllanthus amarus identification. Biol Pharm Bull 33:1723–1727 Google Scholar
Mihalov, JJ, Marderosian, AD, Pierce, JC (2000) DNA identification of commercial ginseng samples. J Agric Food Chem 48:3744–3752 CrossRefGoogle ScholarPubMed
Patterson, DT (1974). The Ecology of Oriental Bittersweet, Celastrus orbiculatus, a Weedy Introduced Ornamental Vine. Ph.D Dissertation. Durham, NC: Duke University. 252 pGoogle Scholar
Pavlovic, NB, Leicht-Young, SA (2011) Are temperate mature forests buffered from invasive lianas? J Torrey Bot Soc 138:85–92 CrossRefGoogle Scholar
Peters, WL, Meyer, MH, Anderson, NO (2006) Minnesota horticultural industry survey on invasive plants. Euphytica 148:75–86 CrossRefGoogle Scholar
R Core Team (2016) R: A Language and Environment for Statistical Computing. Vienna, Austria: R Foundation for Statistical Computing, http://www.R-project.org. Accessed December 1, 2016Google Scholar
Rachmayanti, Y, Leinemann, L, Gailing, O, Finkeldey, R (2009) DNA from processed and unprocessed wood: factors influencing the isolation success. Forensic Sci Int Genet 3:185–192 CrossRefGoogle ScholarPubMed
Reichard, SH, Hamilton, CW (1997) Predicting invasions of woody plants introduced into North America. Conserv Biol 11:193–203 Google Scholar
Schuelke, M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234 Google Scholar
Srirama, R, Senthilkumar, U, Sreejayan, N, Ravikanth, G, Gurumurthy, BR, Shivanna, MB, Sanjappa, M, Ganeshaiah, KN, Shaanker, RU (2010) Assessing species admixtures in raw drug trade of Phyllanthus, a hepato-protective plant using molecular tools. J Ethnopharmacol 130:208–215 Google Scholar
Tallamy, DW (2007) Bringing Nature Home: How Native Plants Sutain Wildlife in Our Gardens. Portland, OR: Timber Press. 288 pGoogle Scholar
[USDA-NRCS] U.S. Department of Agriculture, Natural Resources Conservation Service (2017) The PLANTS Database. National Plant Data Team. http://plants.usda.gov. Accessed June 1, 2017Google Scholar
Vongsak, B, Kengtong, S, Vajrodaya, S, Sukrong, S (2008) Sequencing analysis of the medicinal plant Stemona tuberosa and five related species existing in Thailand based on trnH-psbA chloroplast DNA. Planta Med 74:1764–1766 Google Scholar
Wang, CZ, Li, P, Ding, JY, Peng, X, Yuan, CS (2007) Simultaneous identification of Bulbus Fritillariae Cirrhosae using PCR-RFLP analysis. Phytomedicine 14:628–632 Google Scholar
Whelan, RJ, Roberts, DG, England, PR, Ayre, DJ (2006) The potential for genetic contamination vs. augmentation by native plants in urban gardens. Biol Conserv 128:493–500 CrossRefGoogle Scholar
Williams, PA, Timmins, SM (2003) Climbing spindle berry (Celastrus orbiculatus Thunb.) biology, ecology, and impacts in New Zealand. Wellington, New Zealand: New Zealand Government, Department of Conservation. 28 p Google Scholar
Xue, CY, Li, DZ, Lu, JM, Yang, JB, Liu, JQ (2006) Molecular authentication of the traditional Tibetan medicinal plant Swertia mussotii . Planta Med 72:1223–1226 Google Scholar
Young, JA, Young, CG (1992) Seeds of Woody Plants in North America. Portland, OR: Dioscorides Press. 236 p Google Scholar
Zaya, DN (2013). Genetic Characterization of Invasion and Hybridization: A Bittersweet (Celastrus spp.) Story. Ph.D Dissertation. Chicago, IL: University of Illinois at Chicago. 90 pGoogle Scholar
Zaya, DN, Ashley, MV (2012) Plant genetics for forensic applications. Pages 35–52 in Sucher NJ, Hennell JR, Carles MC, eds. Plant DNA Fingerprinting and Barcoding. New York, NY: Humana Google Scholar
Zaya, DN, Leicht-Young, SA, Pavlovic, NB, Feldheim, KA, Ashley, MV (2015) Genetic characterization of hybridization between native and invasive bittersweet vines (Celastrus spp.). Biol Invasions 17:2975–2988 Google Scholar
Zhu, YY, Chen, HR, Fan, JH, Wang, YY, Li, Y, Chen, JB, Fan, JX, Yang, SS, Hu, LP, Leung, H, Mew, TW, Teng, PS, Wang, ZH, Mundt, CC (2000) Genetic diversity and disease control in rice. Nature 406:718–722 Google Scholar