Hostname: page-component-cd9895bd7-gvvz8 Total loading time: 0 Render date: 2024-12-23T08:57:44.406Z Has data issue: false hasContentIssue false
  • English
  • Français

Variation in the coding and non-coding sequences of the DH-PBAN gene of diapause and non-diapause silkworm races

Published online by Cambridge University Press:  01 June 2008

R. Saravanakumar ,
K.M. Ponnuvel  and
C.K. Kamble
R. Saravanakumar
Affiliation:
Biotechnology Laboratory, Central Sericultural Germplasm Resources Centre, PO Box 44, Thally Road, Hosur635 109, Tamil Nadu, India
K.M. Ponnuvel*
Affiliation:
Biotechnology Laboratory, Central Sericultural Germplasm Resources Centre, PO Box 44, Thally Road, Hosur635 109, Tamil Nadu, India
C.K. Kamble
Affiliation:
Biotechnology Laboratory, Central Sericultural Germplasm Resources Centre, PO Box 44, Thally Road, Hosur635 109, Tamil Nadu, India
*
*E-mail: [email protected]
Get access

Abstract

Diapause hormone (DH) and pheromone biosynthesis-activating neuropeptide (PBAN) code for the diapause gene (DH-PBAN) of Bombyx mori Linnaeus. The DH-PBAN gene sequence from the BLAST database was searched against B. mori genomic gene sequences for similarity. Results showed maximum homology with a genomic contig (accession no. D16230). Primers were designed for the intron, exon and promoter regions of the DH-PBAN gene sequence. The corresponding regions were amplified using genomic DNA as template and products of diapause and non-diapause silkworm races were compared. There was no variation in the 425 bp intron and 254 bp exon regions. However, 1.3% nucleotide sequence variation was observed in selected silkworm races. The promoter region showed 4.2% single nucleotide variation in the diapause and non-diapause silkworm races. The promoter region of the diapause gene showed variations in the sequence of the POU (Pit 1, Oct 1 and Unc-86)-binding site of non-diapause and diapause races of B. mori. In phylogenetic analysis, B. mori formed a cluster with other silkworms of the Bombycidae family, whereas the Noctuidae family formed a separate cluster, indicating that the diapause gene is conserved across the insect taxa.

Keywords

diapauseDH-PBANsilkwormmultivoltinebivoltineBombyx mori
Type
Research Paper
Information
International Journal of Tropical Insect Science , Volume 28 , Issue 2 , June 2008 , pp. 69 - 77
Copyright
Copyright © ICIPE 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Altschul, S. F., Madden, T. L. and Schaffer, A. A. (1997) Gapped BLAST and PSI BLAST: a new generation of protein database search programs. Nucleic Acids Research 25, 3389–3402.Google Scholar
Choi, M. Y., Lee, J. M., Han, K. S. and Boo, K. S. (2004) Identification of a new member of PBAN family and immunoreactivity in the central nervous system from Adoxophyes sp. (Lepidoptera: Tortricidae). Insect Biochemistry and Molecular Biology 34, 927–935.Google Scholar
Choi, M. Y., Tanaka, M., Kataoka, H., Boo, S. K. and Tatsuki, S. (1998) Isolation and identification of the cDNA encoding the pheromone biosynthesis activating neuropeptide and additional neuropeptides in the oriental tobacco budworm, Helicoverpa assulta (Lepidoptera: Noctuidae). Insect Biochemistry and Molecular Biology 28, 759–766.Google Scholar
Denlinger, D. L. (1985) Hormonal control of diapause, pp. 353–412. In Comprehensive Insect Physiology Biochemistry and Pharmacology, Vol. 8 (Edited by Kerkut, G. A. and Gilbert, L. I.). Pergamon Press, Oxford.Google Scholar
Iglesias, F., Marco, P., Francois, M. C., Camps, F., Fabrias, G. and Jacquin-Joly, E. (2002) A new member of the PBAN family in Spodoptera littoralis: Molecular cloning and immunovisualisation in scotophase hemolymph. Insect Biochemistry and Molecular Biology 32, 901–908.Google Scholar
Imai, K., Konno, T., Nakazawa, Y., Komiya, T., Isobe, M., Koga, K., Goto, T., Yaginuma, T., Sakakibara, K., Hasegawa, K. and Yamashita, O. (1991) Isolation and structure of diapause hormone of the silkworm, Bombyx mori. Proceedings of the Japan Academy Series B 67, 98–101.Google Scholar
Jacquin-Joly, E., Burnet, M., Francois, M. C., Ammar, D., Meillour, P. N. and Descoins, C. (1998) cDNA cloning and sequence determination of the pheromone biosynthesis activating neuropeptide of Mamestra brassicae: A new member of the PBAN family. Insect Biochemistry and Molecular Biology 28, 251–258.Google Scholar
Katsuno, S. (1989) Spermatogenesis and the abnormal germ cells in Bombycidae and Saturniidae. Journal of the Faculty of Agriculture, Hokkaido University 64, 21–34.Google Scholar
Krishnaswami, S. (1978) New technology of silkworm rearing. Bulletin No. 2., Central Sericultural Research and Training Institute, Mysore, Central Silk Board, Government of India.Google Scholar
Kumar, S., Tamura, K. and Nei, M. (2004) MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Briefings in Bioinformatics 5, 150–163.Google Scholar
Lees, A. D. (1955) The Physiology of Diapause in Arthropods. Cambridge University Press, Cambridge.Google Scholar
Ma, P. W. K., Knipple, D. C. and Roelofs, W. L. (1994) Structural organization of the Helicoverpa zea gene encoding the precursor protein for pheromone biosynthesis activating neuropeptide and other neuropeptides. Proceedings of the National Academy of Sciences of the USA 91, 6506–6510.Google Scholar
Nagaraja, G. M. and Nagaraju, J. (1995) Genome fingerprinting of the silkworm Bombyx mori using random arbitrary primers. Electrophoresis 16, 1633–1638.Google Scholar
Saravanakumar, R. and Ponnuvel, K. M. (2007) Egg diapause induction in multivoltine silkworm Bombyx mori for long-term germplasm preservation. International Journal of Industrial Entomology 15, 291–297.Google Scholar
Sato, Y., Oguchi, M., Menjo, N., Imai, K., Saito, H., Ikeda, M., Isobe, M. and Yamashita, O. (1993) Precursor polyprotein for multiple neuropeptides secreted from the subesophageal ganglion of the silkworm Bombyx mori: Characterization of the cDNA encoding the diapause hormone precursor and identification of additional peptides. Proceedings of the National Academy of Science USA 90, 3251–3255.Google Scholar
Saunders, D. S. (1982) Insect Clocks, 2nd edn, pp. 137-192. Pergamon Press, Oxford.Google Scholar
Thompson, J. D., Higgins, D. G. and Gibson, T. J. (1994) Clustal W: Improving the sensitivity of progressive multiple sequence alignment through weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Research 22, 4673–4680.Google Scholar
Velu, D., Ponnuvel, K. M., Muthulakshmi, M., Sinha, R. K. and Qadri, S. M. (2008) Analysis of genetic relationship in mutant silkworm strains of Bombyx mori using Inter Simple Sequence Repeat (ISSR) markers. Journal of Genetics and Genomics 35, 291–297.Google Scholar
Watanabe, K., Hull, J. J., Niimi, T., Imai, K., Matsumoto, S., Yaginuma, T. and Kataoka, H. (2007) FXPRL-amide peptides induce ecdysteroidogenesis through a G-protein coupled receptor expressed in the prothoracic gland of Bombyx mori. Molecular and Cellular Endocrinology 273, 51–58.Google Scholar
Wheelan, S. I., Church, D. M. and Ostell, J. M. (2001) SPIDEY—a tool for mRNA to genomic alignments. Genome Research 11, 1952–1957.Google Scholar
Xu, W. H. and Denlinger, D. L. (2003) Molecular characterization of prothoracicotropic hormone and diapause hormone in Heliothis virescens during diapause, and a new role for diapause hormone. Insect Molecular Biology 12, 509–516.Google Scholar
Xu, W. H. and Denlinger, D. L. (2004) Identification of a cDNA encoding DH-PBAN and other FXPRL neuropeptides from the tobacco hornworm, Manduca sexta, and expression associated with pupal diapause. Peptides 25, 1099–1106.Google Scholar
Xu, W. H., Sato, Y., Ikeda, M. and Yamashita, O. (1995) Molecular characterization of the gene encoding the precursor protein of diapause hormone and pheromone biosynthesis activating neuropeptide (DH-PBAN) of the silkworm, Bombyx mori and its distribution in some insects. Biochimica et Biophysica Acta 1261, 83–89.Google Scholar
Yamashita, O. (1996) Diapause hormone of the silkworm, Bombyx mori: Structure, gene expression and function. Journal of Insect Physiology 42, 669–679.Google Scholar
Yamashita, O. and Hasegawa, K. (1985) Embryonic diapause, pp. 407–434. In Comprehensive Insect Physiology, Biochemistry and Pharmacology, Vol. 1 (Edited by Kerkut, C. A. and Gilbert, L. I.). Pergamon Press, Oxford.Google Scholar
Zhang, T. Y., Sun, J. S., Liu, W. Y., Kang, L., Shen, J. L. and Xu, W. H. (2005) Structural characterization and transcriptional regulation of the gene encoding diapause hormone and pheromone biosynthesis activating neuropeptide in the cotton bollworm, Helicoverpa armigera. Biochimica et Biophysica Acta 1728, 44–52.Google Scholar
Zhang, T. Y., Sun, J. S., Zhang, L. B., Shen, J. L. and Xu, W. H. (2004) Cloning and expression of the cDNA encoding the FXPRL family of peptides and a functional analysis of their effect on breaking pupal diapause in Helicoverpa armigera. Journal of Insect Physiology 50, 25–33.CrossRefGoogle Scholar
Zhao, J. Y., Xu, W. H. and Kang, L. (2004) Functional analysis of the SGNP I in the pupal diapause of the oriental budworm, Helicoverpa assulta (Lepidoptera: Noctuidae). Regulatory Peptides 118, 25–31.Google Scholar