Hostname: page-component-586b7cd67f-t8hqh Total loading time: 0 Render date: 2024-11-22T19:28:53.708Z Has data issue: false hasContentIssue false
  • English
  • Français

Studies on the life cycle of five microsporidian isolates and histopathology of the mid-gut of the silkworm Bombyx mori (Lepidoptera: Bombycidae)

Published online by Cambridge University Press:  11 December 2012

Zakir Hossain ,
Sunil Kumar Gupta ,
Satadal Chakrabarty ,
Atul Kumar Saha  and
Bharat Bhushan Bindroo
Zakir Hossain*
Affiliation:
Central Sericultural Research and Training Institute, Central Silk Board, PO Berhampore, District Murshidabad, West Bengal742 101, India
Sunil Kumar Gupta
Affiliation:
P2 Basic Seed Farm, National Silkworm Seed Organization, Central Silk Board, Purnea, Bihar854 301, India
Satadal Chakrabarty
Affiliation:
Central Sericultural Research and Training Institute, Central Silk Board, PO Berhampore, District Murshidabad, West Bengal742 101, India
Atul Kumar Saha
Affiliation:
Central Sericultural Research and Training Institute, Central Silk Board, PO Berhampore, District Murshidabad, West Bengal742 101, India
Bharat Bhushan Bindroo
Affiliation:
Central Sericultural Research and Training Institute, Central Silk Board, PO Berhampore, District Murshidabad, West Bengal742 101, India
*
*E-mail: [email protected]
Get access

Abstract

Pebrine caused by Nosema bombycis in the silkworm Bombyx mori L. causes severe economic loss to the sericulture industry. Several species of microsporidia and strains of N. bombycis have been isolated from infected silkworms. The study of the developmental stages of any parasite is important, as it leads to the identification of stages that may be susceptible to control measures. For this study, five microsporidian isolates from B. mori were collected from five different locations in West Bengal, India and a study of the developmental stages from mid-gut smears and histological techniques was undertaken. The observations of mid-gut smears and histological specimens revealed differences in the morphometry and duration of different developmental stages of the parasites.

Keywords

pebrineNosema bombycisBombyx morimicrosporidiasilkwormmid-gut
Type
Research Article
Information
International Journal of Tropical Insect Science , Volume 32 , Issue 4 , December 2012 , pp. 203 - 209
Copyright
Copyright © ICIPE 2012

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ananthalakshmi, K. V. V., Fujiwara, T. and Dutta, R. K. (1994) First report on the isolation of three microsporidians (Nosema spp.) from the silkworm, Bombyx mori L. in India. Indian Journal of Sericulture 33, 146–148.Google Scholar
Andreadis, T. G. (2007) Microsporidian parasites of mosquitoes. Journal of the American Mosquito Control Association 23, 3–29(bulletin no. 7).Google Scholar
Becnel, J. J. (1992) Horizontal transmission and subsequent development of Amblyospora californica (Microsporidia: Amblyosporidae) in the intermediate and definite hosts. Diseases of Aquatic Organisms 13, 17–28.CrossRefGoogle Scholar
Becnel, J. J. and Andreadis, T. G. (1999) Microsporidia in insects, pp. 447–501. In The Microsporidia and Microsporidiosis (edited by ). ASM Press, Washington, DC.Google Scholar
Canning, E. U. and Lom, J. (1986) The Microsporidia of Vertebrates. Academic Press, New York and London. 289 pp.Google Scholar
Curry, A. and Canning, E. U. (1993) Human microsporidiosis. Journal of Infection 27, 229–236.CrossRefGoogle ScholarPubMed
De Graaf, D. C., Raes, H. and Jacob, J. F. (1994) Spore dimorphism in Nosema apis (Microsporida, Nosematidae) developmental cycle. Journal of Invertebrate Pathology 63, 92–94.CrossRefGoogle Scholar
Fowler, J. L. and Reeves, E. (1975) Microsporidian spore structure as revealed by scanning electron microscopy. Journal of Invertebrate Pathology 26, 1–6.CrossRefGoogle Scholar
Fries, I. (1993) Nosema apis: a parasite in the honey bee colony. Bee World 74, 5–19.CrossRefGoogle Scholar
Fujiwara, T. (1980) Three microsporidians (Nosema spp.) from the silkworm Bombyx mori. Journal of Sericultural Science of Japan 49, 229–236.Google Scholar
Fujiwara, T. (1984) A Pleistophora like microsporidia isolated from the silkworm Bombyx mori. Journal of Sericultural Science of Japan 53, 398–402.Google Scholar
Fujiwara, T. (1985) Microsporidia from silkworm moths in egg production sericulture. Journal of Sericultural Science of Japan 54, 108–111.Google Scholar
Gupta, S. K., Hossain, Z., Mohanan, M., Mitra, P., Mandal, K. and Bajpai, A. K. (2011) Electron microscopic observation of five microsporidia infecting silkworm Bombyx mori L, pp. 173–180. In Recent Trends in Sericulture (edited by ). Narendra Publishing House, Delhi.Google Scholar
Hylis, M., Pilarska, D. K., Obornik, M., Vavra, J., Solter, L. F., Weiser, J., Linde, A. and McManus, M. L. (2006) Nosema chrysorrhoeae n. sp. (Microsporidia), isolated from browntail moth (Euproctis chrysorrhoea L.) (Lepidoptera, Lymantriidae) in Bulgaria: characterisation and phylogenetic relationships. Journal of Invertebrate Pathology 91, 105–114.Google Scholar
Ishihara, R. (1969) The life cycle of Nosema bombycis as revealed in tissue culture cells of Bombyx mori. Journal of Invertebrate Pathology 14, 316–320.Google Scholar
Iwano, H. and Ishihara, R. (1991) Dimorphism of spores of Nosema spp. in cultured cell. Journal of Invertebrate Pathology 57, 211–219.Google Scholar
Jolly, M. S. (1986) Pebrine and Its Control. Central Silk Board Publication, Bangalore.Google Scholar
Kawarabata, T. and Ishihara, R. (1984) Infection and development of Nosema bombycis (Microsporidia: Protozoa) in a cell line of Antheraea eucalypti. Journal of Invertebrate Pathology 44, 52–62.CrossRefGoogle Scholar
Keeling, P. J. (2003) Congruent evidence from alpha-tubulin and betatubulin gene phylogenies for a zygomycete origin of microsporidia. Fungal Genetics and Biology 38, 298–309.Google Scholar
Kramer, J. P. (1965) Nosema necatrix sp. n. and Thelohania diazoma sp. n., microsporidians from the armyworm Pseudaletia unipuncta (Howarth). Journal of Invertebrate Pathology 7, 117–121.CrossRefGoogle Scholar
Orenstein, J. M. (1991) Microsporidiosis in the acquired immunodeficiency syndrome. Journal of Parasitology 77, 843–864.Google Scholar
Rao, S. N., Muthulakshmi, M., Kanginakudru, S. and Nagaraju, J. (2004) Phylogenetic relationships of three new microsporidian isolates from the silkworm, Bombyx mori. Journal of Invertebrate Pathology 86, 87–95.Google Scholar
Sato, R. and Watanabe, H. (1980) Purification of mature microsporidian spores by iso-density equilibrium centrifugation. Journal of Sericultural Science of Japan 49, 512–516.Google Scholar
Solter, L. F. and Becnel, J. J. (2000) Entomopathogenic microsporidia, pp. 231–254. In Field Manual of Techniques for the Evaluation of Entomopathogens (edited by ). Kluwer Academic Publishers, Dordrecht.Google Scholar
Sprague, V. (1981) Microsporidia, pp. 589–594. In Synopsis and Classification of Living Organisms (edited by ). McGraw-Hill, New York, NY.Google Scholar
Sprague, V. and Vernick, S. H. (1971) The ultrastructure of Encephalitozoon cuniculi (Microsporida: Nosematidae) and its taxonomic significance. Journal of Protozoology 18, 566–569.Google Scholar
Vossbrinck, C. R. and Debrunner-Vossbrinck, B. A. (2005) Molecular phylogeny of the Microsporidia: ecological, ultrastructural and taxonomic considerations. Folia Parasitologica 52, 131–142.Google Scholar
Vossbrink, C. R., Maddox, J. V., Friedman, S., DebrunnerVossbrinck, C. and Woese, R. (1987) Ribosomal RNA sequence suggests microsporidia are extremely ancient eukaryotes. Nature 326, 411–414.Google Scholar
Wittner, M. and Weiss, L. M. (1999) The Microsporidia and Microsporidiosis. ASM Press, Washington, DC. 553 pp.Google Scholar