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Screening and bioassay of entomopathogenic fungi for the control of silverleaf whitefly, Bemisia argentifolli

Published online by Cambridge University Press:  19 September 2011

Adhanom Negasi ,
Bruce L. Parker  and
Michael Brownbridge
Adhanom Negasi
Affiliation:
Entomology Research Laboratory, University of Vermont, P. O. Box 53400, Burlington, VT 05405–3400
Bruce L. Parker
Affiliation:
Entomology Research Laboratory, University of Vermont, P. O. Box 53400, Burlington, VT 05405–3400
Michael Brownbridge
Affiliation:
Entomology Research Laboratory, University of Vermont, P. O. Box 53400, Burlington, VT 05405–3400
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Abstract

Isolates of Beauveria bassiana (Balsamo) Vuillemin, Paecilomyces farinosus (Holm ex SF Gray) Brown and Smith and Verticillium lecanii (Zimmermann) Viegas were evaluated for their pathogenicity to adults, eggs, first and third instars of silverleaf whitefly, Bemisia argentifolli (Bellows and Perring) (Homoptera: Aleyrodidae). Conidial dilutions were prepared in 0.05% Tween® and leaves treated by immersion in these suspensions. Assessments of nymphal infection were made 2, 3, 5, 7, and 10 days post-treatment. Infection rates in adults and eggs were made 14 days post-treatment. All isolates were pathogenic to nymphs and adults. None of the isolates were pathogenic to eggs. Isolates L3444 (P. farinosus) and L3009 (B. bassiana) were the most pathogenic to first instars and adults with LC50 and LC90 values of 7.3 × 104 and 3.1 × 106 conidia/ml, and 1.3 × 106 and 1.0 × 107 conidia/ml, respectively. Isolate FR20 (V. lecanii) was the most pathogenic to third instars with LC50 and LC90 values of 4.8 × 103 and 7.4 × 105 conidia/ml, respectively. Results imply that L3444 and L3009 are the most efficient isolates, based on their pathogenicity to first instars and adults. Results are discussed in relation to using fungi for management of silverleaf whitefly in greenhouses.

Keywords

Bemisia argentifollibioassaybiological controlentomopathogenic fungifungal isolatesBemisia argentifolliessai biologiquelutte biologiquechampignons entomopathogènesisolats fongiques
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 18 , Issue 1 , March 1998 , pp. 37 - 44
Copyright
Copyright © ICIPE 1998

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