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Pathogenicity and Histopathology of a Nuclear Polyhedrosis Virus of Spodoptera litura (Fab.)

Published online by Cambridge University Press:  19 September 2011

Dae Joon Im ,
B. M. Shepard  and
R. M. Aguda
Dae Joon Im
Affiliation:
Department of Entomology, Agricultural Sciences Institute, Rural Development Administration, Suwon, Korea
B. M. Shepard
Affiliation:
Department of Entomology, Agricultural Sciences Institute, Rural Development Administration, Suwon, Korea
R. M. Aguda
Affiliation:
Department of Entomology, International Rice Research Institute, College, Los Baños, Laguna, Philippines
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Abstract

Third instar larvae of Spodoptera litura (Fab.) treated with polyhedrosis virus at dosages above 3.2 × 107 polyhedra inclusion bodies (PIB)/ml produced over 90% mortality. The LC50 was 2.3 × 104 PIB/ml. The estimated median lethal time (LT50) was 7.4 and 6 days at the lowest and highest dosages, respectively.

Polyhedra were observed in the epidermal cells, tracheal matrix, muscles and all nuclei of tissues of the virus treated larva 48–72 hr after treatment.

Résumé

Une mortalité supérieure à 90% a été observée sur des larves du troisième stade de Spodoptera litura (Fab.) traitées avec des virus polygonaux nucléaires à des concentrations supérieures à 3, 2 × 107 corps polyédriques/ml.

La DC50 est de 2,3 × 104 corps polyédriques/ml. L'estimation de la médiane du temps de léthalité est de 7,4 jours pour le dosage maximum et 6 jours pour le dosage minimum.

Les corps polyédriques ont été observés 48 a 72 heures aprés traitement aves le virus dans les cellules épidermiques, la trachée, les muscles et tous les noyaux des tissus des larves.

Keywords

Spodoptera lituranuclear polyhedrosis virushistopathologypathogenicity
Type
Research Article
Information
International Journal of Tropical Insect Science , Volume 9 , Issue 4 , August 1988 , pp. 539 - 542
Copyright
Copyright © ICIPE 1988

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References

REFERENCES

Chauthani, A. R., Claussen, D. and Rehnborg, C. S. (1968) Dosage mortality data on a nuclear polyhedrosis virus of Heliothis zea. J. Invertebr. Pathol. 12, 335–338.Google Scholar
Hamm, J. J. (1968) Comparative histopathology of a granulosis and a nuclear polyhedrosis of Spodoptera frugiperda. J. lnvertebr. Pathol. 10, 320–326.CrossRefGoogle Scholar
Im, D. J., Aguda, R. M. and Shepard, B. M. (1986) A granulosis virus of the rice brown semi-looper, Mocis frugalis (Fab.) (Lepidoptera: Noctuidae). Int. Rice Res. Newsl. 11, 33.Google Scholar
Isa, A. L. and Khadr, G. D. (1973) Rearing of Ostrinia nubilalis Hbn. on artificial diets. Agric. Res. Rev. 51, 15–19.Google Scholar
Kirschbaum, J. B. (1985) Potential implication of genetic engineering and other biotechnologies to insect control. A. Rev. Ent. 30, 51–70.CrossRefGoogle ScholarPubMed
Legacion, D. M. and Gabriel, B. P. (1975) Dosage mortality study on the nuclear polyhedrosis virus of the common cutworm. Spodoptera litura (Fab.) (Lepidoptera: Noctuidae). Philipp. Ent. 3, 109–116.Google Scholar
Magnoler, A. (1974) Bioassay of a nucleopolyhedrosis virus of the gypsy moth, Porthetria dispar. J. lnvertebr. Pathol. 23, 190–196.CrossRefGoogle ScholarPubMed
Mathad, S. B., Splittstoesser, C. M. and McEwen, F. L. (1968) Histopathology of the cabbage looper, Trichoplusia ni, infested with a nuclear polyhedrosis. J. Invertebr. Pathol. 11, 456–464.CrossRefGoogle Scholar
Miller, L. K., Lingg, A. J. and Bulla, L. A. (1983) Bacterial, viral and fungal insecticides. Science 219, 715–721.CrossRefGoogle ScholarPubMed
Okada, T. (1970) A histopathological study on the nuclear polyhedrosis of larva of Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae). Kyushu Agric. Exp. Stn. Bull. 16, 40–41. (In Japanese).Google Scholar
Ossowski, L. L. J. (1960) Variation in virulence of a wattle bagworm virus. J. Insect Pathol. 2, 35–43.Google Scholar
Smirnoff, W. A. (1961) A virus disease of Neodiprion swainei Middeton. J. Insect Pathol. 3, 29–6.Google Scholar
Whitlock, V. H. (1978) Dosage-mortality studies of a granulosis and a nuclear polyhedrosis virus of a laboratory strain of Heliothis armigera. J. Invertebr. Pathol. 32, 384–387.CrossRefGoogle Scholar
Yearian, W. C. and Young, S. Y. (1982) Control of insect pests of agricultural importance by viral insecticides. In Microbial and Viral Pesticides (Edited by Kurstak, E.), pp. 387–423. Basel, Marcell, Dekker, Inc., New York.Google Scholar