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Mosquito Vitellin: Structural and Functional Studies with Monoclonal Antibodies

Published online by Cambridge University Press:  19 September 2011

Manthri S. Ramasamy ,
Michael Sands ,
Julie Gale  and
Ranjan Ramasamy
Manthri S. Ramasamy
Affiliation:
Queensland Institute of Medical Research, Bramston Terrace, Brisbane 4006, Australia
Michael Sands
Affiliation:
Queensland Institute of Medical Research, Bramston Terrace, Brisbane 4006, Australia
Julie Gale
Affiliation:
Queensland Institute of Medical Research, Bramston Terrace, Brisbane 4006, Australia
Ranjan Ramasamy
Affiliation:
Queensland Institute of Medical Research, Bramston Terrace, Brisbane 4006, Australia
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Abstract

Two monoclonal antibodies (Mabs), that react in an enzyme linked immunoabsorbant assay, were generated against Aedes aegypti vitellin. One of these Mabs, VIIG9 that reacted predominantly with a 64 kDa component of Ae. aegypti yolk protein, also recognized 68 kDa and 66 kDa components in the yolk proteins of Culex sitiens and Cx. annulirostris, respectively. Mab VIIG9 has been used to detect vitellin or vitellin precursors in gravid female and non-blood fed nulliparous female mosquitoes as a possible age grading method. The presence of polyclonal and monoclonal antibodies to vitellin in the blood meal did not affect the fecundity of Ae. aegypti.

Résumé

Deux anticorps monoclones (Mabs), qui reagissent dans un essai enzyme lie et immunoabsorbant, ont ete generes contre Aedes aegypti vitelline. L'un de ces Mabs, V11G9 qui a reagi d'une maniere predominante avec un constituant 64 kDa de proteine de vitellus de Ae. aegypti a egalement reconnu les constituants 68kDa et 66 kDa dans les proteines de vitellus de Culex sitiens et de Cx. annulirostris respectivement. On s'est servi de Mab VIIG9 pour decouvrir la vitelline ou les precurseurs de la vitelline dans des femelles pleines et des moustiques femelles non nourries de sang et nullipares comme une methode possible de classer l'age. La presence d'anticorps polyclones et monoclones a la vitelline dans le sang absorbe n'a pas eu d'effect sur la fecondite de Ae. aegypti.

Keywords

Aedes aegyptiAnopheles farautiCulex annulirostrisCx. sitiensvitellinmonoclonal antibodyfecundityAedes aegyptiAnopheles farautiCulex annulirostrisCx. sitiensvitellineanticorps monoclonalfecondite
Type
Research Article
Information
International Journal of Tropical Insect Science , Volume 9 , Issue 4 , August 1988 , pp. 499 - 504
Copyright
Copyright © ICIPE 1988

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