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Isoenzyme characterization of the tick Rhipicephalus appendiculatus Neumann (Acarina: Ixodidae) in Kenya

Published online by Cambridge University Press:  19 September 2011

S. S. Kubasu ,
A. S. Young  and
G. R. Karuhize
S. S. Kubasu
Affiliation:
National Museums of Kenya, Department of Invertebrate Zoology, Entomology Section, P. O. Box 40658, Nairobi, Kenya
A. S. Young
Affiliation:
Protozoology Division, National Veterinary Research Centre, Muguga, Kenya Agricultural Research Institute, P. O. Box 32, Kikuyu, Kenya
G. R. Karuhize
Affiliation:
Department of Zoology, University of Nairobi, P. O. Box 30197, Nairobi, Kenya
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Abstract

Five populations of the tick, Rhipicephalus appendiculatus (collected from Kilifi, South Nyanza, and Uasin Gishu districts, and a Muguga laboratory strain) were subjected to isoenzyme analysis. Isoelectric focusing (IEF) of 11 enzymes on agarose gels was used to determine genetic differences between the tick populations. The isoenzyme profile results of the 11 enzymes investigated indicated that four loci, peptidase 1 (PEP 1:EC 3.4.11.1), PEP 2:EC 3.4.11.1, malate dehydrogenase (MDH:EC 1.1.1.37) and glucose phosphate isomerase (GPI:EC 5.3.1.9) showed differences in the populations. The Uasin Gishu population was distinct from all others. Ten out of the eleven enzymes studied were polymorphic in most of the populations while only one (i.e. PEP 7) was monomorphic.

Résumé

Cinq populations de Rhipicephalus appendiculatus provenant de Kilifi, de Kiambu, de South Nyanza, des districts de Uasin Gishu et du laboratoire de Muguga, ont été soumises à des analyses isoenzymatiques. Différentes populations génetiques de tiques ont été observées à partir du point isoelectrique sur gel d'agar agar de onze enzymes. Les profiles isoenzymatiques des onze enzymes ont montré que 4 loci, peptidase 1 (PEP 1: EC 3.4.11.1), PEP 2: EC 3.4.11.1, malate dehydrogenase (MDH: EC 1.1.1.37) et glucose phosphate isomerase (GPI: EC 5.3.1.9) témoignent de l'existence de populations hétérogènes; la population de Uasin Gishu étant distincte de toutes les autres. Dix des onze enzymes étudiés étaient polymorphiques pour toutes les populations alors que la PEP 7 se trouvait être monomorphique.

Keywords

Tick populationsisoenzymesisoelectric focusingpolymorphismzymograms
Type
Tick Immunology
Information
International Journal of Tropical Insect Science , Volume 13 , Special Issue 4: Status and Recent Advances in Tick Management in Africa , August 1992 , pp. 475 - 480
Copyright
Copyright © ICIPE 1992

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