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Improvement of techniques for mass-culture of Chilo partellus (Swinhoe)

Published online by Cambridge University Press:  19 September 2011

R. S. Ochieng ,
F. O. Onyango  and
M. D. O. Bungu
R. S. Ochieng
Affiliation:
The International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi, Kenya
F. O. Onyango
Affiliation:
The International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi, Kenya
M. D. O. Bungu
Affiliation:
The International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi, Kenya
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Abstract

A method for steady production of Chilo partellus has been established. An efficient simple and easy method of collection of first instar larvae from incubation chambers has been developed. It has been established that pupae of Chilo partellus can be held at a chilling temperature between 5 and 15°C without any adverse effect on the emergence of adults. This finding has been used in synchronizing the emergence of males and females for mating or longer storage of the pupae.

Studies on the effect of high and low humidities on egg development have shown that low humidities affect the hatchability of Chilo partellus egg but did not effect the actual development of the embryo adversely. It has also been shown that Chilo partellus oviposits best under high humidity.

Résumé

Une methode de stabilisation de production du Chilo partellus a été établie. Une méthode efficace, simple et facile pour recueillir les premiers instars des larves, des chambres d'incubation a été développée. Il a été établie que la pupe du Chilo partellus peut être tenue a des températures très froides entre 5 et 15°C sans aucun effet défavorable sur l'émergence des insectes. Cette décourverte a été utilisée dans la synchronisation de l'émergence des mâles et femelles pour accouplement ou pour une conservation plus longue de la pupe. Des études sur l'effet d'une humidité basse ou élevée sur le développement de l'oeuf ont montré que une basse humidité affecte l'éclosion de l'oeuf du Chilo partellus mais n'affecte pas défavorablement le développement de l'embryon. Il a été aussi montré que Chilo partellus ovipose mieux dans des conditions d'humidité élevée.

Keywords

Chilo partellusmass-culturebase-ingredientsresistanceartificial dietPénicilliumFusariumAspergillusbenomylChilo partellusculture massiveingrédients de base résistancerégime artificielPenicillumFusaricumAspergillusbenomyl
Type
Section V: screening techniques and methodologies for host plant resistance research
Information
International Journal of Tropical Insect Science , Volume 6 , Special Issue 3: Host Plant Resistance and its Significance in Pest Management , June 1985 , pp. 425 - 428
Copyright
Copyright © ICIPE 1985

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References

REFERENCES

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