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Identification of genes related to resin biosynthesis in the Indian lac insect, Kerria lacca (Hemiptera: Tachardiidae)

Published online by Cambridge University Press:  29 May 2014

Gulsaz Shamim ,
D.M. Pandey ,
R. Ramani  and
K.K. Sharma
Gulsaz Shamim
Affiliation:
Department of Biotechnology, Birla Institute of Technology, Mesra, Ranchi835 215, Jharkhand, India Indian Institute of Natural Resins and Gums, Namkum, Ranchi834 010, Jharkhand, India
D.M. Pandey
Affiliation:
Department of Biotechnology, Birla Institute of Technology, Mesra, Ranchi835 215, Jharkhand, India
R. Ramani*
Affiliation:
Indian Institute of Natural Resins and Gums, Namkum, Ranchi834 010, Jharkhand, India
K.K. Sharma
Affiliation:
Indian Institute of Natural Resins and Gums, Namkum, Ranchi834 010, Jharkhand, India
*
*E-mail: [email protected]
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Abstract

Kerria lacca (Kerr) is commercially harnessed for lac resin, which is principally an ester complex of aleuritic acid (9,10,16-trihydroxyhexadecanoic acid) and jalaric acid. The present study is an attempt made to identify the possible pathways involved in the biosynthesis of lac resin. It is proposed that acetyl-CoA is the common precursor for the biosynthesis of aleuritic acid and sesquiterpenic acids (jalaric acid). Prenyltransferases are involved in the biosynthesis of sesquiterpenes; hydroxylation of hexadecanoic acid, after chain elongation, appears to occur through the action of cytochrome P450 enzymes. Two related genes as proposed above were identified and sequenced. The diurnal rhythm of resin secretion and protein concentrations were also studied and correlated for ascertaining the active secretory phase.

Keywords

lac insectsaleuritic acidsesquiterpeneslac resinjalaric acidbiosynthesis
Type
Research Papers
Information
International Journal of Tropical Insect Science , Volume 34 , Issue 2 , June 2014 , pp. 149 - 155
Copyright
Copyright © ICIPE 2014 

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