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Host immunoglobulin G in the haemolymph of the brown ear tick, Rhipicephalus appendiculatus (Neumann, 1901)

Published online by Cambridge University Press:  19 September 2011

S. K. Mbogo ,
E. O. Osir  and
A. O. Mongi
S. K. Mbogo
Affiliation:
Kenya Agricultural Research Institute, National Veterinary Research Centre, P. O. Box 32, Kikuyu
E. O. Osir
Affiliation:
The International Centre of Insect Physiology and Ecology (ICIPE), P. O. Box 30772, Nairobi
A. O. Mongi
Affiliation:
The International Centre of Insect Physiology and Ecology (ICIPE), P. O. Box 30772, Nairobi
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Abstract

Adult female Rhipicephalus appendiculatus ticks were fed on rabbits immunized with either bovine serum albumin, solubilized tick midgut membrane proteins, a 50:50 mixture of bovine serum albumin and solubilized tick midgut membrane proteins or buffer. Haemolymph harvested from these ticks after they had fed to engorgement and sera from the immunized rabbits were assayed for the presence and quantity of both the total rabbit IgG and specific rabbit IgG raised against bovine serum albumin using the enzyme linked immunosorbent assay technique. The results obtained indicated that rabbit IgG was able to cross the midgut barrier of the tick into the haemolymph. Furthermore, the amounts of IgG crossing into the haemolymph were dependent on the antibody titres in the serum. The results also showed that feeding ticks on blood containing anti-tick midgut antibodies increased the passage of IgG into the haemolymph.

Résumé

Des tiques Rhipicephalus appendiculatus femelles ont été nourries sur des lapins immunisés à partir soit d'albumine sérique bovin, soit de proteines solubles issues de la membrane intestinale de tiques, soi d'un mélange à égale des deux précédentes substances ou à partir d'une solution tampon. Il a été procédé a moyen de la technique de l'ELISA au dosage de l'IgG des lapins dans l'hémolymphe des tiques de même qu'à l'estimation de la quantité d'hemoglobine secrétée par les lapins contre les substances à eux injectées. Les résultats obtenus, indiquent que l'IgG des lapins peut traverser la barrière intestinale de la tique et se retrouver dans l'hemolymphe.Ils indiquent aussi que le taux d'IgG traversant la barrière intestinale de la tique dépend du taux d'anticorps sérique. Ces résultats de la même manière indiquent que la prise de nourriture par des tiques sur des animaux immuns contre des proteines de tiques d'origine intestinale endommage les structures intestinales et augmente le passage des anticorps de l'intestine à l'hémolymphe.

Keywords

Enzyme linked immunosorbent assayimmunizationIgG in tick haemolymphRhipicephalus appendiculatussolubilized tick midgut membrane proteinsRhipicephalus appendiculatusimmunoglobulines G dans l'hémolymphe de tiquesELISAproteines solubles obtenues a partir de la membrane intestinale de tiquesimmunisation
Type
Tick Immunology
Information
International Journal of Tropical Insect Science , Volume 13 , Special Issue 4: Status and Recent Advances in Tick Management in Africa , August 1992 , pp. 481 - 485
Copyright
Copyright © ICIPE 1992

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