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Exploration for Triatoma virus (TrV) infection in laboratory-reared triatomines of Latin America: a collaborative study*

Published online by Cambridge University Press:  05 November 2013

Gerardo A. Marti ,
María G. Echeverría ,
María L. Susevich ,
Soledad Ceccarelli ,
Agustín Balsalobre ,
… Jorge E. Rabinovich ,
Lileia Diotaiuti  and
Diego M.A. Guérin
Gerardo A. Marti
Affiliation:
Centro de Estudios Parasitológicos y de Vectores (CEPAVE-CCT-La Plata-CONICET-UNLP), Calle 2 Nro. 584, 1900La Plata, Argentina
María G. Echeverría
Affiliation:
Cátedra de Virología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata (UNLP-CONICET), Av. 60 y 117, 1900La Plata, Argentina
María L. Susevich
Affiliation:
Centro de Estudios Parasitológicos y de Vectores (CEPAVE-CCT-La Plata-CONICET-UNLP), Calle 2 Nro. 584, 1900La Plata, Argentina Cátedra de Virología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata (UNLP-CONICET), Av. 60 y 117, 1900La Plata, Argentina
Soledad Ceccarelli
Affiliation:
Centro de Estudios Parasitológicos y de Vectores (CEPAVE-CCT-La Plata-CONICET-UNLP), Calle 2 Nro. 584, 1900La Plata, Argentina
Agustín Balsalobre
Affiliation:
Centro de Estudios Parasitológicos y de Vectores (CEPAVE-CCT-La Plata-CONICET-UNLP), Calle 2 Nro. 584, 1900La Plata, Argentina
…  Jorge E. Rabinovich
Affiliation:
Centro de Estudios Parasitológicos y de Vectores (CEPAVE-CCT-La Plata-CONICET-UNLP), Calle 2 Nro. 584, 1900La Plata, Argentina
Lileia Diotaiuti
Affiliation:
Centro de Pesquisas René Rachou-FIOCRUZ, Laboratório de Triatomíneos e Epidemiologia da Doença de Chagas, Avenida Augusto de Lima, 1715, Barro Preto, Belo Horizonte, MG, CEP 30190-002Minas Gerais, Brazil
Diego M.A. Guérin*
Affiliation:
Unidad de Biofísica (CSIC-UPV/EHU), Barrio Sarriena s/n, 48940 Leioa, Bizkaia, Spain Fundación Biofísica Bizkaia, Barrio Sarriena s/n, 48940 Leioa, Bizkaia, Spain Departamento de Bioquímica y Biología Molecular, Facultad de Ciencia y Tecnología, Universidad del País Vasco (EHU), Barrio Sarriena s/n, 48940Leioa, Bizkaia, Spain
*
*E-mail: [email protected] (L. Diotaiuti); [email protected] (D.M.A. Guérin)
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Abstract

Triatoma virus (TrV) is a small, non-enveloped virus that has a+ssRNA genome and is currently classified under the Cripavirus genus of the Dicistroviridae family. TrV infects haematophagous triatomine insects (Hemiptera: Reduviidae), which are vectors of American trypanosomosis (Chagas disease). TrV can be transmitted through the horizontal faecal–oral route, and causes either deleterious sublethal effects or even the death of laboratory insect colonies. Various species of triatomines from different regions of Latin America are currently being reared in research laboratories, with little or no awareness of the presence of TrV; therefore, any biological conclusion drawn from experiments on insects infected with this virus is inherently affected by the side effects of its infection. In this study, we developed a mathematical model to estimate the sample size required for detecting a TrV infection. We applied this model to screen the infection in the faeces of triatomines belonging to insectaries from 13 Latin American countries, carrying out the identification of TrV by using RT-PCR. TrV was detected in samples coming from Argentina, which is where the virus was first isolated from Triatoma infestans (Hemiptera: Reduviidae) several years ago. Interestingly, several colonies from Brazil were also found infected with the virus. This positive result widens the TrV's host range to a total of 14 triatomine species. Our findings suggest that many triatomine species distributed over a large region of South America may be naturally infected with TrV.

Keywords

DicistroviridaeTriatoma virussampling modelinsectary contaminationpathogen transmissionChagas diseaseRedTrV
Type
Multi-Institutional Study
Information
International Journal of Tropical Insect Science , Volume 33 , Issue 4 , December 2013 , pp. 294 - 304
Copyright
Copyright © Published by icipe 2013 

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