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Effect of pH, rearing temperature and sunlight on infectivity of Malaysian isolate of nucleopolyhedrovirus to larvae of Spodoptera litura (Lepidoptera: Noctuidae)

Published online by Cambridge University Press:  01 June 2007

A.S. Sajap*
Affiliation:
Department of Forest Management, Universiti Putra Malaysia, 43400, UPM, Serdang, Selangor, Malaysia
M.A. Bakir
Affiliation:
Department of Forest Management, Universiti Putra Malaysia, 43400, UPM, Serdang, Selangor, Malaysia
H.A. Kadir
Affiliation:
Malaysian Agricultural Research Development Institute, PO Box 12341, Kuala Lumpur, Malaysia
N.A. Samad
Affiliation:
Department of Biochemistry, Universiti Putra Malaysia, 43400, UPM, Serdang, Selangor, Malaysia
*
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Abstract

The effects of pH, sunlight and rearing temperature on infectivity of nucleopolyhedrovirus (SpltNPV) were investigated against larvae of Spodoptera litura (Fabricius). The infectivity of virus increased with increased larval rearing temperature. The LT50 values were significantly reduced from 24 to 6 days when rearing temperature was increased from 20 to 35 °C. The pH of the substrate significantly affected the infectivity of the virus. The LT50 values increased to about fourfold when the pH of suspension was raised from 7 to 11. Infectivity of the virus was more adversely affected in alkaline than in acidic suspension. Sunlight also deleteriously affected infectivity of the virus. The LT50 values increased with the increase in the period of sunlight exposure. A complete viral inactivation occurred after 12 h of direct sunlight exposure.

Type
Research Paper
Copyright
Copyright © ICIPE 2007

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