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Cuticular lesions: a non-infectious integumental disease of Glossina species

Published online by Cambridge University Press:  19 September 2011

M. O. Odindo ,
D. A. Turner ,
W. A. Otieno  and
G. P. Kaaya
M. O. Odindo
Affiliation:
ICIPE, Coastal Field Station, P.O. Box 80804, Mombasa, Kenya
D. A. Turner
Affiliation:
ICIPE, P.O. Box 30772, Nairobi, Kenya
W. A. Otieno
Affiliation:
ICIPE, Coastal Field Station, P.O. Box 80804, Mombasa, Kenya
G. P. Kaaya
Affiliation:
ICIPE, P.O. Box 30772, Nairobi, Kenya
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Abstract

Purified suspensions of macerated cuticular lesions from the insectary-reared tsetse Glossina morsitans morsitans were applied on laboratory-reared newly emerged teneral tsetse by three pathways: topical application on the ventral abdominal integument, oral microfeeding, or microinjection into the haemocoel. A second group of flies were subjected to teratological wounding pin-stabs, forcep-pinch and shallow blade-slits, all in attempt to induce cuticular lesions. Samples of tsetse were examined every week for 11 weeks. Application of cuticular lesion suspensions did not result in lesion formation, but three types of lesions were formed due to physical damage induced artificially on the integument.

Total haemocyte counts (THCs) and differential haemocyte counts (DHCs) were also determined for tsetse with, and without, lesions. THCs were significantly higher (P < 0.01) in tsetse with cuticular lesions than in those without lesions. DHCs showed a high number of large spindle-shaped cells and thrombocytoids in the flies with lesions (P < 0.05).

Keywords

Glossina morsitans morsitanscuticular lesionsnon-infectious diseaseteratologytotal haemocyte countdifferential haemocyte countwound healing process
Type
Research Article
Information
International Journal of Tropical Insect Science , Volume 2 , Issue 4 , December 1981 , pp. 213 - 217
Copyright
Copyright © ICIPE 1981

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