Hostname: page-component-586b7cd67f-rdxmf Total loading time: 0 Render date: 2024-11-26T05:18:57.888Z Has data issue: false hasContentIssue false

The composition of the salivary gland secretion of the tsetse, Glossina morsitans morsitans Westwood 1850 (Diptera: Glossinidae)

Published online by Cambridge University Press:  19 September 2011

Nilu Y. Patel
Affiliation:
International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi, Kenya
Anthony Youdeowei
Affiliation:
International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi, Kenya
Thomas R. Odhiambo
Affiliation:
International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi, Kenya
Get access

Abstract

Polyacrylamide gel disc electrophoresis of the salivary secretion from the tsetse, Glossina morsitans morsitans Westwood, showed 11 protein bands, four of which were characterized as glycoproteins. Sixteen amino acids and four sugars in the hydrolysates of saliva were identified. Thin layer chromatography showed five lipid classes and three phospholipids. The secretion is a clear, transparent, viscous fluid with a pH between 7.00 and 7.5. These findings are discussed in relation to the nutritional requirements of the metacyclic forms of trypanosomes that may be found in the salivary glands.

Type
Research Article
Copyright
Copyright © ICIPE 1981

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Baptis, T. B. A. (1941) The morphology and physiology of the salivary glands of Hemiptera-Hepteroptera. Q. Jl mierosc. Sci. 83, 311319.Google Scholar
Burtt, E. (1946) Salivation of Glossina morsitans on the glass slides: a technique for isolating flies. Ann. trop. Med. Parasit. 40, 141.CrossRefGoogle ScholarPubMed
Chayen, J., Bitenwesky, L. and Butcher, R. G. (1973) Practical Histochemistry. John Wiley, London.Google Scholar
Cross, G. A. M. (1975) Identification, purification and properties of clone-specific glycoprotein antigens constituting the surface coat of T. brucei. Parasitology 71, 389–418.CrossRefGoogle Scholar
Davies, B. J. (1964) Disc electrophoresis—II. Method and application to human serum proteins. Ann. N. Y. Acad. Sci. 121, 404–427.CrossRefGoogle Scholar
D'Costa, M. A. and Rice, M. J. (1973) Localization and quantification of glycogen in the tsetse-fly Glossina morsitans and the blow-fly Chrysoma putoria. Comp. Biochem. Physiol. 45B, 483–485.Google Scholar
Diezel, W., Kipperschilärger, G. and Hoffman, E. (1972) An improved procedure for protein staining in polyacrylamide gels with a new type of Coomassie Brilliant Blue. Analyt. Biochem. 48, 617–620.CrossRefGoogle ScholarPubMed
Fairbairn, H. and Williamson, J. (1956) The composition of tsetse fly saliva—I. A histochemical analysis. Ann. trop. Med. Parasit. 50, 322–333.CrossRefGoogle ScholarPubMed
Maurer, H. R. (1971) Disc Electrophoresis and Related Techniques of Polyacrylamide Gel Electrophoresis. Walter de Gruyter, Berlin.Google Scholar
Olembo, N. K. (1972) M.Sc. thesis, University of Nairobi.Google Scholar
Partridge, S. M. (1948) Filter paper partition chromatography of sugars. 2: an examination of the blood group A specific substances from hog gastric mucin, and the specific polysaccharide of Bacterium dysenteriae (Shiga). Biochem. J. 42, 251–253.CrossRefGoogle ScholarPubMed
Pearse, A. G. E. (1961) Histochemistry: Theoretical and Applied. J. and A. Churchill, London.Google Scholar
Smith, I. (1960) Chromatographic and Electrophoretic Techniques. Interscience, New York.Google Scholar
Toledo, F. S. A. and de Magalhaes, L. E. (1973) Electroferese em gel amido netodoes para a detecao de isoenzimas en dipteros. Ciênc. Cult. S Paulo 25, 1148–1153.Google Scholar
Trevelyan, W. E., Proctor, D. P. and Harrison, J. S. (1950) Detection of sugars on paper chromatograms. Nature 116, 144–145.Google Scholar
Whitemore, E. and Gilbert, L. I. (1974) Haemolymph proteins and lipo-proteins in Lepidoptera. A comparative electrophoreticstudy. Comp. Biochem. Physiol. 47B, 63.Google Scholar
Wigglesworth, V. B. (1929) Digestion in tsetse fly. A study of structure and function. Parasitology 21, 288–321.CrossRefGoogle Scholar
Wigglesworth, V. B. (1931) Digestion in Chrysops silacae Aust. (Diptera, Tabanidae). Parasitology 23, 83–76.CrossRefGoogle Scholar
Williamson, J. (1956) The composition of tsetse-fly saliva —II. Analysis of amino acids and sugars by paper partition chromatography. Ann. trop. Med. Parasit. 50, 334–344.CrossRefGoogle ScholarPubMed
Youdeowei, A. (1975) A simple technique for observing and collecting the saliva of tsetse flies (Diptera, Glossinidae). Bull. ent. Res. 65, 6567.CrossRefGoogle Scholar