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Characteristics and virulence of nucleopolyhedrovirus isolated from Hyposidra talaca (Lepidoptera: Geometridae), a pest of tea in Darjeeling Terai, India

Published online by Cambridge University Press:  01 June 2011

Ananda Mukhopadhyay ,
Sangita Khewa  and
Damayanti De
Ananda Mukhopadhyay*
Affiliation:
Entomology Research Unit, Department of Zoology, University of North Bengal, Darjeeling734 013, India
Sangita Khewa
Affiliation:
Entomology Research Unit, Department of Zoology, University of North Bengal, Darjeeling734 013, India
Damayanti De
Affiliation:
Entomology Research Unit, Department of Zoology, University of North Bengal, Darjeeling734 013, India
*
*E-mail: [email protected]
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Abstract

Nucleopolyhedrovirus (NPV) was isolated from infected Hyposidra talaca (Walker) caterpillars. This defoliating pest was sampled from the Terai and Dooars tea plantations along the foothills of Darjeeling, India. Phase contrast and transmission electron microscopy revealed polyhedral occlusion bodies (OBs) typical of NPVs. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis analysis showed that the molecular weight of the major protein of the OBs is 32.17 kDa. Dose–mortality bioassays conducted for OBs on the second instar caterpillars of H. talaca resulted in a median lethal concentration (LC50) of 2.8 × 103 OBs/ml. The median lethal time (LT50) was 5.45 days for 1 × 104 OBs/ml, 4.15 days for 1 × 105 OBs/ml and 4.05 days for 1 × 106 OBs/ml concentrations. These results indicate the potential of using this NPV as a microbial pesticide against H. talaca.

Keywords

Hyposidra talacanucleopolyhedrovirusCamellia sinensisDarjeeling
Type
Research Paper
Information
International Journal of Tropical Insect Science , Volume 31 , Issue 1-2 , June 2011 , pp. 13 - 19
Copyright
Copyright © ICIPE 2011

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