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Use of the entomopathogenic fungi Metarhizium anisopliae, Cordyceps bassiana and Isaria fumosorosea to control Diaphorina citri (Hemiptera: Psyllidae) in Persian lime under field conditions

Published online by Cambridge University Press:  12 March 2012

Roberto Lezama-Gutiérrez ,
Jaime Molina-Ochoa ,
Omar Chávez-Flores ,
César Andrés Ángel-Sahagún ,
Steven R. Skoda ,
Gerardo Reyes-Martínez ,
Marisela Barba-Reynoso ,
Oscar Rebolledo-Domínguez ,
Graciela M.L. Ruíz-Aguilar  and
John E. Foster
Roberto Lezama-Gutiérrez
Affiliation:
Facultad de Ciencias Biológicas y Agropecuarias, Universidad de Colima, Apartado Postal 36, Km. 40 autopista Colima-Manzanillo, Tecomán, Colima28930, Mexico
Jaime Molina-Ochoa*
Affiliation:
División Ciencias de la Salud e Ingenierías, Departamento Agroindustrial, Universidad de Guanajuato, Campus Celaya-Salvatierra, Salvatierra, Guanajuato38900, Mexico Department of Entomology, Insect Genetics Laboratory, University of Nebraska-Lincoln, 3B Entomology Hall, Lincoln, NE68583-0816, USA
Omar Chávez-Flores
Affiliation:
Facultad de Ciencias Biológicas y Agropecuarias, Universidad de Colima, Apartado Postal 36, Km. 40 autopista Colima-Manzanillo, Tecomán, Colima28930, Mexico
César Andrés Ángel-Sahagún
Affiliation:
División de Ciencias de la Vida, Departamento de Agronomía, Campus Irapuato-Salamanca, Universidad de Guanajuato, exhacienda El Copal, Km. 7, Carretera Irapuato-Silao, Apartado Postal 311, Irapuato, Guanajuato, CP 36500, Mexico
Steven R. Skoda
Affiliation:
USDA-ARS, Knipling-Bushland U.S. Livestock Insects Research Laboratory, Kerrville, TX78028, USA
Gerardo Reyes-Martínez
Affiliation:
Consejo Estatal de Productores de Limón (COEPLIM), Abasolo 475, Colonia Centro, Tecomán, Colima, CP 28100, Mexico
Marisela Barba-Reynoso
Affiliation:
Consejo Estatal de Productores de Limón (COEPLIM), Abasolo 475, Colonia Centro, Tecomán, Colima, CP 28100, Mexico
Oscar Rebolledo-Domínguez
Affiliation:
Facultad de Ciencias Biológicas y Agropecuarias, Universidad de Colima, Apartado Postal 36, Km. 40 autopista Colima-Manzanillo, Tecomán, Colima28930, Mexico
Graciela M.L. Ruíz-Aguilar
Affiliation:
División Ciencias de la Salud e Ingenierías, Departamento Agroindustrial, Universidad de Guanajuato, Campus Celaya-Salvatierra, Salvatierra, Guanajuato38900, Mexico
John E. Foster
Affiliation:
Department of Entomology, Insect Genetics Laboratory, University of Nebraska-Lincoln, 3B Entomology Hall, Lincoln, NE68583-0816, USA
*
*E-mail: [email protected]; [email protected]
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Abstract

The Asian citrus psyllid Diaphorina citri Kuwayama is a destructive insect pest in citriculture, because it is an efficient vector of the proteobacteria ‘Candidatus Liberibacter asiaticus’ (Las), ‘Ca. L. africanus’ (Laf) and ‘Ca. L. americanus’ (Lam). These bacteria cause the ‘huanglongbing’ disease or ‘greening’ or ‘yellow dragon’ disease. The disease kills the plant and reduces fruit production. This insect pest is susceptible to entomopathogenic fungi, and we report the use of different strains of Metarhizium anisopliae, Cordyceps bassiana and Isaria fumosorosea against the nymphs and adults of D. citri under field conditions. The fungi were applied four times using a concentration of 2 × 1013 conidia/ha with a time interval of 15 days between applications. The percentage of control of Cb 108, Ma 65, Ma 14 and Ifr 4 was 60, 50, 40 and 35% in nymphs, and 50, 50, 42 and 22% in adults, respectively. Metarhizium anisopliae, C. bassiana and I. fumosorosea applied on Persian lime groves are more effective in reducing higher density of nymphs than adults of D. citri.

Keywords

Diaphorina citrihuanglongbingPersian limeMexican limebiological control
Type
Research Paper
Information
International Journal of Tropical Insect Science , Volume 32 , Issue 1 , March 2012 , pp. 39 - 44
Copyright
Copyright © ICIPE 2012

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References

Aubert, B. (1987) Trioza erytreae Del Guercio and Diaphorina citri Kuwayama (Hemiptera: Psylloidea), the two vectors of citrus greening disease: biological aspects and possible control strategies. Fruits 42, 149–162.Google Scholar
Avery, P. B., Hunter, W. B., Hall, D. G., Jackson, M. A., Powell, C. A. and Rogers, M. E. (2009) Diaphorina citri (Hemiptera: Psyllidae) infection and dissemination of the entomopathogenic fungus Isaria fumosorosea (Hypocreales: Cordycipitaceae) under laboratory conditions. Florida Entomologist 92, 608–618.Google Scholar
Batool, A., Iftikhar, Y., Mughal, S. M., Khan, M. M. and Jaskani, M. J. (2007) Citrus greening disease – a major cause of citrus decline in the world – a review. Horticultural Science (Prague) 34, 159–166.Google Scholar
Bellis, G., Hollis, D. and Jacobson, S. (2005) Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae), and huanglongbing disease do not exist in the Stapleton Station area of the Northern Territory of Australia. Australian Journal of Entomology 44, 68–70.Google Scholar
Bové, J. M. (2006) Huanglongbing: a destructive, newly-emerging, century-old disease of citrus. Journal of Plant Pathology 88, 7–37.Google Scholar
Étienne, J., Quilici, S., Marival, D. and Franck, A. (2001) Biological control of Diaphorina citri (Hemiptera: Psyllidae) in Guadeloupe by imported Tamarixia radiata (Hymenoptera: Eulophidae). Fruits 56, 307–315.CrossRefGoogle Scholar
Grafton-Cardwell, E. E., Godfrey, K. E., Rogers, M. E., Childers, C. C. and Stansly, P. A. (2006) Asian citrus psyllid. University of California, Division of Agriculture and Natural Resources, Publication 8205, Oakland, California, 8 pp. Available at:http://anrcatalog.ucdavis.edu/pdf/8205.pdf.CrossRefGoogle Scholar
Halbert, S. E. and Manjunath, K. L. (2004) Asian citrus psyllids (Sternorrhyncha: Psyllidae) and greening disease of citrus: a literature review and assessment of risk in Florida. Florida Entomologist 87, 330–353.CrossRefGoogle Scholar
Lacey, L. A. and Shapiro-Ilan, D. I. (2008) Microbial control of insect pests in temperate orchard systems: potential for incorporation into IPM. Annual Review of Entomology 53, 121–144.Google Scholar
McFarland, C. D. and Hoy, M. A. (2001) Survival of Diaphorina citri (Homoptera: Psyllidae) and its two parasitoids, Tamarixia radiata (Hymenoptera: Eulophidae) and Diaphorencyrtus aligarhensis (Hymenoptera: Encyrtidae), under different relative humidities and temperature regimes. The Florida Entomologist 84, 227–233.CrossRefGoogle Scholar
Meyer, J. M., Hoy, M. A. and Boucias, D. G. (2007) Morphological and molecular characterization of a Hirsutella species infecting the Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae) in Florida. Journal of Invertebrate Pathology 95, 101–109.CrossRefGoogle ScholarPubMed
Meyer, J. M., Hoy, M. A. and Boucias, D. G. (2008) Isolation and characterization of an Isaria fumosorosea isolate infecting the Asian citrus psyllid in Florida. Journal of Invertebrate Pathology 99, 96–102.CrossRefGoogle ScholarPubMed
Michaud, J. P. and Grant, A. K. (2003) IPM-compatibility of foliar insecticides for citrus: indices derived from toxicity to beneficial insects from four orders. Journal of Insect Science 3, 18.CrossRefGoogle ScholarPubMed
Moore, J. (2008) Resistance isn't futile. Florida Growers 101, 22–23.Google Scholar
Prior, C. and Jollands, P. (1988) Infectivity of oil and water formulations of Beauveria bassiana (Deuteromycotina: Hyphomycetes) to the cocoa weevil pest Pantorhytes plutus (Coleoptera: Curculionidae). Journal of Invertebrate Pathology 52, 66–72.Google Scholar
Rivero-Aragón, A. and Grillo-Ravelo, H. (2000) Natural enemies of Diaphorina citri Kuwayama (Homoptera: Psyllidae) in the central region of Cuba. Centro-Agricola 27, 87–88.Google Scholar
SAS Institute (1997) SAS/STAT. User's Guide, release 6.10 edn. SAS Institute, Inc., Cary, North Carolina.Google Scholar
Skelley, L. H. and Hoy, M. A. (2004) A synchronous rearing method for the Asian citrus psyllid and its parasitoid in quarantine. Biological Control 29, 14–23.CrossRefGoogle Scholar
Subandiyah, S., Nikoh, N., Tsuyumu, S., Somowiyarjo, S. and Fukatsu, T. (2000) Complex endosymbiotic microbiota of the citrus psyllid Diaphorina citri (Homoptera: Psylloidea). Zoological Science 17, 983–989.Google Scholar
Tsai, J. H., Wang, J. J. and Liu, Y. H. (2000) Sampling of Diaphorina citri (Homoptera: Psyllidae) on orange Jessamine in southern Florida. Florida Entomologist 83, 446–459.Google Scholar