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Screening of tropical isolates of Metarhizium anisopliae (Hypocreales: Clavicipitaceae) for virulence to the sweet potato weevil, Cylas formicarius (Coleoptera: Brentidae)

Published online by Cambridge University Press:  28 August 2015

Ronnie Dotaona ,
Bree A.L. Wilson ,
Mark M. Stevens ,
Joanne Holloway  and
Gavin J. Ash
Ronnie Dotaona*
Affiliation:
Graham Centre for Agricultural Innovation (an alliance between Charles Sturt University and NSW Department of Primary Industries), Charles Sturt University, Locked Bag 588, Wagga Wagga, NSW 2678, Australia
Bree A.L. Wilson
Affiliation:
Graham Centre for Agricultural Innovation (an alliance between Charles Sturt University and NSW Department of Primary Industries), Charles Sturt University, Locked Bag 588, Wagga Wagga, NSW 2678, Australia
Mark M. Stevens
Affiliation:
Graham Centre for Agricultural Innovation (an alliance between Charles Sturt University and NSW Department of Primary Industries), Yanco Agricultural Institute, NSW Department of Primary Industries, Private Mail Bag, Yanco, NSW 2703, Australia
Joanne Holloway
Affiliation:
Graham Centre for Agricultural Innovation (an alliance between Charles Sturt University and NSW Department of Primary Industries), Wagga Wagga Agricultural Institute, NSW Department of Primary Industries, Private Mail Bag, Wagga Wagga, NSW 2650, Australia
Gavin J. Ash
Affiliation:
Graham Centre for Agricultural Innovation (an alliance between Charles Sturt University and NSW Department of Primary Industries), Charles Sturt University, Locked Bag 588, Wagga Wagga, NSW 2678, Australia
*
*E-mail: [email protected]
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Abstract

The sweet potato weevil (SPW), Cylas formicarius, is a serious pest of sweet potato in Australia and Papua New Guinea. Ten strains of Metarhizium sp. isolated from Australian soil samples were evaluated for their growth characteristics and screened for virulence to adult SPW under laboratory conditions. All isolates except QD62 (48.6%) had moderate to high germination (66–97%), and all took 2 to 4 days to sporulate at 25 °C. The optimal temperature for radial growth for the majority of isolates was 30 °C, and there was a significant interaction between isolate and temperature (P< 0.05). Isolate QS155 showed the fastest radial growth at 30 °C. The internal transcribed spacer sequences showed slight variations among the isolates; however, all isolates were shown to be Metarhizium anisopliae. Isolates varied greatly in their virulence. At 10 days after inoculation (DAI) by immersion in a suspension of 1 × 107conidia/ml, 9 of the 10 isolates were virulent, causing 80–100% mortality of adult SPW. Only two isolates (QS001-6 and QS155) caused more than 50% mortality at 5 DAI. In dose-mortality bioassays, isolate QS155 had the lowest 20-day LC50 and LC90 values; however, there were no statistically significant differences in mortality among the three most promising isolates tested (QD66, QS001-6 and QS155). These results show that M. anisopliae isolate QS155 has potential as a microbial control agent for SPW, and that further evaluation under glasshouse and field conditions is warranted.

Keywords

Sweet potatoCylas formicariusITSMetarhizium anisopliaebioassaymortality
Type
Research Papers
Information
International Journal of Tropical Insect Science , Volume 35 , Issue 4 , December 2015 , pp. 153 - 163
Copyright
Copyright © ICIPE 2015 

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References

Abbott, W. S. (1925) A method of computing the effectiveness of an insecticide. Journal of Economic Entomology 18, 265–267.CrossRefGoogle Scholar
Allsopp, P. G., McGill, N. G., Licastro, K. A. and Milner, R. J. (1994) Control of larvae of Antitrogus consanguineus (Blackburn) (Coleoptera: Scarabaeidae) by injection of Metarhizium anisopliae conidia into soil. Austral Entomology 33, 199–201. doi:10.1111/j.1440-6055.1994.tb01216.x .CrossRefGoogle Scholar
Al-Samarrai, T. H. and Schmid, J. (2000) A simple method for extraction of fungal genomic DNA. Letters in Applied Microbiology 30, 53–56.Google Scholar
Ansari, M. A. and Butt, T. M. (2012) Susceptibility of different developmental stages of large pine weevil Hylobius abietis (Coleoptera: Curculionidae) to entomopathogenic fungi and effect of fungal infection to adult weevils by formulation and application methods. Journal of Invertebrate Pathology 111, 33–40. doi:10.1016/j.jip.2012.05.006 .Google Scholar
Arthurs, S. and Thomas, M. B. (2001) Effects of temperature and relative humidity on sporulation of Metarhizium anisopliae var. acridum in mycosed cadavers of Schistocerca gregaria. Journal of Invertebrate Pathology 78, 59–65.CrossRefGoogle ScholarPubMed
Arzumanov, T., Jenkins, N. and Roussos, S. (2005) Effect of aeration and substrate moisture content on sporulation of Metarhizium anisopliae var. acridum. Process Biochemistry 40, 1037–1042.Google Scholar
Barchia, I. (2001) Probit analysis and fiducial limits in Genstat, p. 3. In Genstat 2001 Conference Programme and Abstracts (edited by Dogan, V., Mayer, D. and Swain, T.). 31 January to 2 February 2001, Mercure Resort, Surfers Paradise, Gold Coast, Australia.Google Scholar
Bischoff, J. F., Rehner, S. A. and Humber, R. A. (2006) Metarhizium frigidum sp. nov.: a cryptic species of M. anisopliae and a member of the M. flavoviride complex. Mycologia 98, 737–745.Google Scholar
Boothe, S. R., Tanigoshi, L. and Dewes, I. (2000) Potential of a dried mycelium formulation of an indigenous strain of Metarhizium anisopliae against subterranean pests of cranberry. Biocontrol Science and Technology 10, 659–668. doi:10.1080/095831500750016451Google Scholar
Bourke, R. M. and Ramakrishna, A. (2009) Sweetpotato in highland agricultural systems of Papua New Guinea, pp. 7–11. In Soil Fertility in Sweetpotato-based Cropping Systems in the Highlands of Papua New Guinea (edited by Kirchhof, G.). ACIAR Technical Report 71, ACIAR, Canberra.Google Scholar
Bruck, D. J. (2004) Natural occurrence of entomopathogens in Pacific Northwest nursery soils and their virulence to the black vine weevil, Otiorhynchus sulcatus (F.) (Coleoptera: Curculionidae). Environmental Entomology 33, 1335–1343.Google Scholar
Coleman, E. A., Hughes, M. J., Jackson, G. V. H., Komolong, B. and Guaf, E. (2009) Genetics and disease as factors in the yield decline of sweetpotato in the Papua New Guinea highlands, pp. 33–42. In Soil Fertility in Sweet Potato-based Cropping Systems in the Highlands of Papua New Guinea (edited by Kirchhof, G.). ACIAR Technical Report 71, ACIAR, Canberra.Google Scholar
Collins, P. J., Treverrow, N. L. and Lambkin, T. M. (1991) Organophosphorus insecticide resistance and its management in the banana weevil borer, Cosmopolites sordidus (Germar) (Coleoptera: Curculionidae), in Australia. Crop Protection 10, 215–221.Google Scholar
Ekesi, S. and Maniania, N. K. (2000) Susceptibility of Megalurothrips sjostedti developmental stages to Metarhizium anisopliae and the effects of infection on feeding, adult fecundity, egg fertility and longevity. Entomologia Experimentalis et Applicata 94, 229–236. doi:10.1046/j.1570-7458.2000.00624.x.Google Scholar
Ekesi, S., Maniania, N. K. and Ampong-Nyarko, K. (1999) Effect of temperature on germination, radial growth and virulence of Metarhizium anisopliae and Beauveria bassiana on Megalurothrips sjostedti. Biocontrol Science and Technology 9, 177–185. doi:10.1080/09583159929767 .Google Scholar
Finney, D. J. (1971) Probit Analysis. Cambridge University Press, UK. 333 pp.Google Scholar
Goettel, M., Inglis, G. and Wraight, S. (2000) Fungi, pp. 255–282. In Field Manual of Techniques in Invertebrate Pathology: Application and Evaluation of Pathogens for Control of Insects and other Invertebrate Pests (edited by Lacey, L. A. and Kaya, H. K.), Vol.1. Kluwer Academic Publishers, Dordrecht, The Netherlands.CrossRefGoogle Scholar
Hajek, A. E., Lund, J. and Smith, M. T. (2008) Reduction in fitness of female Asian longhorned beetle (Anoplophora glabripennis) infected with Metarhizium anisopliae. Journal of Invertebrate Pathology 98, 198–205. doi:10.1016/j.jip.2007.12.003 .Google Scholar
Hughes, M. J., Coleman, E. A., Taraken, T. I. and Igua, P. (2009) Sweetpotato agronomy in Papua New Guinea, pp. 12–23. In Soil Fertility in Sweetpotato-based Cropping Systems in the Highlands of Papua New Guinea (edited by G. Kirchhof). ACIAR Technical Report 71, ACIAR, Canberra.Google Scholar
Inglis, G. D., Duke, G. M., Goettel, M. S. and Kabaluk, J. T. (2008) Genetic diversity of Metarhizium anisopliae var. anisopliae in southwestern British Columbia. Journal of Invertebrate Pathology 98, 101–113. doi:10.1016/j.jip.2007.12.001 .Google Scholar
Lomer, C. J., Bateman, R. P., Johnson, D. L., Langewald, J. and Thomas, M. (2001) Biological control of locusts and grasshoppers. Annual Review of Entomology 46, 667–702.CrossRefGoogle ScholarPubMed
Milner, R. J. (1997) Prospects for biopesticides for aphid control. Entomophaga 42, 227–239.Google Scholar
Moorhouse, E. R., Gillespie, A. T. and Charnley, A. K. (1993) Application of Metarhizium anisopliae (Metsch.) Sor. conidia to control Otiorhynchus sulcatus (F.) (Coleoptera: Curculionidae) larvae on glasshouse pot plants. Annals of Applied Biology 122, 623–636. doi:10.1111/j.1744-7348.1993.tb04063.x .Google Scholar
Ondiaka, S., Maniania, N. K., Nyamasyo, G. H. N. and Nderitu, J. H. (2008) Virulence of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae to sweet potato weevil Cylas puncticollis and effects on fecundity and egg viability. Annals of Applied Biology 153, 41–48.Google Scholar
Prior, C. and Arura, M. (1985) The infectivity of Metarhizium anisopliae to two insect pests of coconuts. Journal of Invertebrate Pathology 45, 187–194.Google Scholar
Quesada-Moraga, E., Navas-Cortés, J. A., Maranhao, E. A., Ortiz-Urquiza, A. and Santiago-Alvarez, C. (2007) Factors affecting the occurrence and distribution of entomopathogenic fungi in natural and cultivated soils. Mycological Research 111, 947–966.Google Scholar
Raeder, U. and Broda, P. (1985) Rapid preparation of DNA from filamentous fungi. Letters in Applied Microbiology 1, 17–20. doi:10.1111/j.1472-765X.1985.tb01479.x.Google Scholar
Rana, R. L. and Villacarlos, L. T. (1991) Effect of Metarhizium anisopliae (Metch:) Sorokin infection on the fecundity and survival of the sweet potato weevil, Cylas formicarius (Fabr.) (Coleoptera: Curculionidae). Philippine Entomologist 8, 963–972.Google Scholar
Robertson, J. L., Russell, R. M., Preisler, H. K. and Savin, N. E. (2007) Bioassays with Arthropods 2 edn.CRC Press, Boca Raton, Florida. 224 pp.Google Scholar
Sar, S. A. (2006) The use of sticky traps to study seasonal dispersal activity of the sweet potato weevil, Cylas formicarius (Fabricius), in Papua New Guinea, pp. 31–35. In Pest and Disease Incursions: Risks, Threats and Management in Papua New Guinea (edited by Price, T. V.). ACIAR, Canberra.Google Scholar
St. Leger, R. J., May, B., Allee, L. L., Frank, D. C., Staples, R. C. and Roberts, D. W. (1992) Genetic differences in allozymes and in formation of infection structures among isolates of the entomopathogenic fungus Metarhizium anisopliae. Journal of Invertebrate Pathology 60, 89–101.Google Scholar
Su, C. Y., Tzean, S. S. and Ko, W. H. (1988) Beauveria bassiana as the lethal factor in a Taiwanese soil pernicious to sweet potato weevil, Cylas formicarius. Journal of Invertebrate Pathology 52, 195–197. doi:10.1016/0022-2011(88)90125-5 .Google Scholar
Sun, J., Fuxa, J. R. and Henderson, G. (2003) Effects of virulence, sporulation, and temperature on Metarhizium anisopliae and Beauveria bassiana laboratory transmission in Coptotermes formosanus. Journal of Invertebrate Pathology 84, 38–46.Google Scholar
Sutherland, J. A. (1986 a) A review of the biology and control of the sweetpotato weevil Cylas formicarius (Fab.). Tropical Pest Management 32, 304–315.Google Scholar
Sutherland, J. A. (1986 b) Damage by Cylas formicarius Fab. to sweet potato vines and tubers, and the effect of infestations on total yield in Papua New Guinea. Tropical Pest Management 32, 316–323. doi:10.1080/09670878609371085 .Google Scholar
Tamura, K., Stecher, G., Peterson, D., Filipski, A. and Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution 30, 2725–2729. doi:10.1093/molbev/mst197 .Google Scholar
Theunis, W. and Aloali'i, I. (1998) Selection of a highly virulent fungal isolate, Metarhizium anisopliae Ma TB 101, for control of taro beetle, Papuana uninodis (Coleoptera: Scarabaeidae). Biocontrol Science and Technology 8, 187–195.Google Scholar
Vänninen, I. (1996) Distribution and occurrence of four entomopathogenic fungi in Finland: effect of geographical location, habitat type and soil type. Mycological Research 100, 93–101.Google Scholar
Vänninen, I., Tyni-Juslin, J. and Hokkanen, H. (2000) Persistence of augmented Metarhizium anisopliae and Beauveria bassiana in Finnish agricultural soils. Biocontrol 45, 201–222.Google Scholar
Wamalwa, L. N., Cheseto, X., Ouna, E., Kaplan, F., Maniania, N. K., Machuka, J., Torto, B. and Ghislain, M. (2014) Toxic ipomeamarone accumulation in healthy parts of sweetpotato (Ipomoea batatas L. Lam) storage roots upon infection by Rhizopus stolonifer. Journal of Agriculture and Food Chemistry 63, 335–342.Google Scholar
White, T. J., Bruns, T., Lee, S. and Taylor, J. W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, pp. 315–322. In PCR Protocols: A Guide to Methods and Applications (edited by Innis, M. A., Gelfand, D. H., Sninsky, J. J. and White, T. J.). Academic Press, New York.Google Scholar
Yeo, H., Pell, J. K., Alderson, P. G., Clark, S. J. and Pye, B. J. (2003) Laboratory evaluation of temperature effects on the germination and growth of entomopathogenic fungi and on their pathogenicity to two aphid species. Pest Management Science 59, 156–165.Google Scholar
Zar, J. H. (1984) Biostatistical Analysis 2 edn.Prentice-Hall Inc., Englewood Cliffs, NJ.Google Scholar
Zimmerman, G. (1986) The ‘Galleria bait method’ for detection of entomopathogenic fungi in soil. Journal of Applied Entomology 102, 213–215.Google Scholar