Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-26T06:18:53.919Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of Trypanosoma brucei infection on the salivary gland secretions of the tsetse Glossina morsitans morsitans (Westwood)

Published online by Cambridge University Press:  19 September 2011

N. Y. Patel ,
L. H. Otieno  and
T. K. Golder
N. Y. Patel
Affiliation:
International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi, Kenya
L. H. Otieno
Affiliation:
International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi, Kenya
T. K. Golder
Affiliation:
International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi, Kenya
Get access

Abstract

Polyacrylamide gel electrophoresis of saliva infected with Trypanosoma brucei showed few, very faint protein bands as compared to 11 bands from clean saliva. If the saliva collected is 6 times the normal amount, infected saliva showed all 11 bands, plus one more. Inositol and galactosamine did not appear in infected saliva. Infected saliva contained sphingomyelin in addition to phosphatidylethano-lamine, phosphatidylinositol and phosphatidyllecithin.

Keywords

Tsetsesalivary glandsalivaTrypanosoma brucei metacyclicspolyacrylamide gel electrophoresis
Type
Articles
Information
International Journal of Tropical Insect Science , Volume 3 , Issue 1 , March 1982 , pp. 35 - 38
Copyright
Copyright © ICIPE 1982

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Davis, B. J. (1964) Disc electrophoresis—II. Method and application to human serum proteins. Ann. N.Y. Acad. Sci. 121, 404–427.CrossRefGoogle ScholarPubMed
Diezel, W. K. and Hoffman, G. E. (1972) An improved procedure for protein staining in polyacryamide gels. Ann. Biochem. 48, 617–620.CrossRefGoogle Scholar
Fairbairn, H. and Williamson, J. (1956) The composition of the tsetse fly saliva—I. A histochemical analysis. Ann. trop. Med. Parasit. 50, 322–333.Google Scholar
Geigy, R. and Kauffman, M. (1964) On the effect of substances found in Glossina tissues on culture trypanosomes of the Bnicei-subgroup. Acta trop. 21, 169–173.Google Scholar
Langreth, S. G. and Balber, A. E. (1975) Protein uptake and digestion in blood stream and culture forms of Trypanosoma brucei. J. Protozool. 22, 40–53.CrossRefGoogle Scholar
Nyindo, M., Patel, N. Y., Darji, N. and Golder, T. K. (1979) Trypanosoma brucei: in vitro preparation of metacyclic forms derived from the salivary glands of Glossina morsitans. J. Parasit. 65, 751–755.Google Scholar
Partridge, S. M. (1948) Filter paper partition chromatography of sugars. Biochem. J. 42, 251–253.CrossRefGoogle ScholarPubMed
Patel, N. Y., Youdeowei, A. and Odhiambo, T. R. (1980) The composition of the salivary gland secretion of the tsetse, Glossina morsitans morsitans Westwood 1850 (Diptera:Glossinidae). Insect Sci. Application 1, 383–387.Google Scholar
Steiger, R. (1971) Some aspects of the surface coat formation in Trypanosoma brucei. Acta trop. 28, 341–346.Google Scholar
Steiger, R. (1973) On the ultrastructure of Trypanosoma (Trypanozoon) brucei in the course of its life cycle and some related aspects. Acta trop. 30, 63–168.Google ScholarPubMed
Trevelyan, W. E., Proctor, D. P. and Harrison, J. S. (1950) Detection of sugars on paper chromatograms. Nature, Lond. 166, 144–145.Google Scholar
Youdeowei, A. (1975) A simple technique for observation and collecting the saliva of tsetse flies (Diptera, Glossinidae). Bull ent. Res. 65, 65–67.Google Scholar
Williams, R. J., Eakin, R. E., Beerstecher, E. Jr, and Shive, W. (1950) The Biochemistry of B Vitamins. Reinhold, New York.CrossRefGoogle Scholar