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Effect of pouterin, a protein from Pouteria torta (Sapotaceae) seeds, on the development of Anagasta kuehniella (Lepidoptera: Pyralidae)

Published online by Cambridge University Press:  01 March 2009

Ana Paula de A. Boleti ,
Carlos Eduardo G. Kubo  and
Maria Lígia R. Macedo
Ana Paula de A. Boleti
Affiliation:
Departamento de Bioquímica/IB, Universidade Estadual de Campinas, PO Box 6109, 13083-970Campinas, SP, Brazil
Carlos Eduardo G. Kubo
Affiliation:
Laboratório de Purificação de Proteínas e Suas Funções Biológicas, Depto de Tecnologia de Alimentos e Saúde Pública, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso do Sul, CP 549, CEP 79070-900, Campo Grande, MS, Brazil
Maria Lígia R. Macedo*
Affiliation:
Departamento de Bioquímica/IB, Universidade Estadual de Campinas, PO Box 6109, 13083-970Campinas, SP, Brazil Laboratório de Purificação de Proteínas e Suas Funções Biológicas, Depto de Tecnologia de Alimentos e Saúde Pública, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso do Sul, CP 549, CEP 79070-900, Campo Grande, MS, Brazil
*
*E-mail: [email protected]
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Abstract

Larvae of the Mediterranean flour moth (Anagasta kuehniella Zeller) cause major losses in stored grains throughout the world. In this study, pouterin, a lectin-like protein isolated from Pouteria torta (Mart.) Radlk. seeds, was tested for activity against A. kuehniella larvae. Pouterin did not produce significant effects on survival; however, at c. 1% w/w concentration in artificial diet, it produced a 71.4% reduction in average weight of the larvae. A dietary utilisation assay showed a reduction in efficiency of conversion of ingested food, efficiency of conversion of digested food and approximate digestibility, as well as an increase in metabolic cost. Pouterin increased the level of trypsin in the midgut and faeces of larvae. We conclude that pouterin possesses an insecticidal effect against A. kuehniella larvae and this effect may be mediated by the binding of pouterin to chitin components of the peritrophic membrane, or glycosylated proteins in the insect midgut.

Keywords

Anagasta kuehniellapouterinPouteria tortastorage pesttoxicitynutritional indices
Type
Research Paper
Information
International Journal of Tropical Insect Science , Volume 29 , Issue 1 , March 2009 , pp. 24 - 30
Copyright
Copyright © ICIPE 2009

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